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  4. Recombinant Rat GTPase KRas (Kras)

Recombinant Rat GTPase KRas (Kras)

  • 货号：
    CSB-YP012493RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012493RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012493RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012493RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012493RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Kras; Kras2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras) [Cleaved into: GTPase KRas; N-terminally processed]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-186
  • 氨基酸序列
    MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QELARSYGIP FIETSAKTRQ RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner.
  • 基因功能参考文献：
    1. Kras protein mutation is responsible for the development of mucinous adenocarcinoma of the lung in patient born with congenital pulmonary malformation. PMID: 27645976
    2. Taken together, these results reveal the key role of histone H3Kme in TGF-beta1 mediated p21 gene expression in RMC, partly through HMT SET7/9 occupancy, suggesting H3Kme and SET7/9 may be potential renoprotective agents in managing chronic renal diseases. PMID: 27247942
    3. Study provided evidence of antiapoptotic effect of miR-30b in early phase of rat myocardial I/R injury model via the Ras/Akt pathway and showed KRAS as a target involved in miR-30b mediated anti-apoptotic effect. PMID: 25925903
    4. VEGF/VEGFR/K-ras signaling pathway might promote the occurrence and development of hepatocellular carcinoma cells through regulating expression of miRNA21, which has potential clinical value for the development of therapies for hepatocellular carcinoma PMID: 25730004
    5. Findings demonstrated that oncogenic K-Ras activates the signaling cascade p38/PDPK1/PKCdelta/p47(phox)/NOX1 for ROS generation and consequent malignant cellular transformation. PMID: 24632950
    6. Piper betle may act as a potential chemopreventive agent in the early stage of colon carcinogenesis by suppressing the expressions of beta-catenin and KRAS. PMID: 24617038
    7. beta-cell apoptosis can be triggered by p21 during stress and is thus a potential target to inhibit for protection of functional beta-cell mass. PMID: 23592481
    8. Oncogenic K-Ras modulates expression and localization of catenin and HDAC proteins in Rat2 cells. PMID: 23747726
    9. Data indicate that Ras-Raf-ERK-Sp1 signaling pathway is responsibe for FGF-2-induced nestin expression. PMID: 23611784
    10. mitochondrial functional loss may be mediated by oncogenic K-Ras-induced mitophagy during early tumorigenesis even in the absence of hypoxia PMID: 21738012
    11. It appears that p21Ras induces asthmatic ASMCs to a proliferative/secretory phenotype, but its inhibitor FTP III, can significantly reverse this phenotype. PMID: 21362304
    12. Vascular smooth muscle cell proliferation is influenced by let-7d microRNA and its interaction with KRAS. PMID: 21266788
    13. limited clinical usefulness of gene mutation as a selection marker for epidermal growth factor receptor tyrosine-kinase inhibitors sensitivity in non-small cell lung cancer PMID: 20022659
    14. The proto-oncogenic k-Ras is directly involved in the promotion of muscle differentiation via PI3K and its downstream signaling pathways. PMID: 20603646
    15. Reactive oxygen species, Ki-Ras, and mitochondrial superoxide dismutase cooperate in nerve growth factor-induced differentiation of PC12 cells PMID: 20495008
    16. Genome-wide gene expression analysis identifies K-ras as a regulator of alcohol intake. The data implicate K-ras-regulated pathways in the regulation of excessive alcohol drinking after a history of dependence PMID: 20388501
    17. N-nitroso-N-methylurea disrupted and dietary methylselenocysteine significantly increased the expression of p21 in mammary glands of Fischer 344 rats PMID: 20424134
    18. study shows social isolation decreases content of Bcl-2-associated K-Ras in hippocampal mitochondria but amount of H-Ras is increased in the microsomal fraction; redistribution of Ras isoforms was accompanied by acceleration in mitochondrial hexokinase PMID: 19616040
    19. SCOP bound through its LRR domain with a subset of K-ras in the raft fraction, interfering with the binding of added guanine nucleotide to K-Ras. PMID: 12594205
    20. AGEs signal to Ki-Ras and ERK through reactive oxygen species-dependent activation of PI3K. PMID: 12871951
    21. Ki-ras activation via mutations at codons 12 and 13 is neither a constant event nor an early event in the development of rat thyroid follicular-cell carcinoma. PMID: 15053237
    22. Low frequency of NRAS and KRAS2 gene mutations in childhood myelodysplastic syndromes. PMID: 15474158
    23. H-Ras was the major mediator of ERK-induced proliferation and survival, whereas H-Ras and K-Ras both mediated PI3K-induced survival. PMID: 18163378
    24. Differential interference of chlorpromazine with the membrane interactions of oncogenic K-Ras and its effects on cell growth. PMID: 18693247
    25. Transformation by oncogenic Ras expands the early genomic response to transforming growth factor beta in intestinal epithelial cells. PMID: 18813357
    26. Epithelial cells expressing oncogenic K-Ras showed increased proliferation mediated through COX-2-dependent activation of beta-catenin signaling and inhibition of apoptosis. PMID: 19037103
    27. (18)F-FDG uptake in the thyroid carcinoma cell line ML-1 is no longer regulated by TSH or cAMP or mediated by GLUT-1. PMID: 19652218

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol.; [Isoform 2B]: Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Small GTPase superfamily, Ras family
  • 数据库链接：

    KEGG: rno:24525

    STRING: 10116.ENSRNOP00000012588

    UniGene: Rn.24554