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  4. Recombinant Rat Glucokinase (Gck)

Recombinant Rat Glucokinase (Gck)

  • 货号：
    CSB-YP009319RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009319RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009319RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009319RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009319RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    GckHexokinase-4; HK4; EC 2.7.1.1; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-D
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-465
  • 氨基酸序列
    MLDDRARMEA TKKEKVEQIL AEFQLQEEDL KKVMSRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEAGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDLD KGILLNWTKG FKASGAEGNN IVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDRQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRMVDES SANPGQQLYE KIIGGKYMGE LVRLVLLKLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DSGDRKQIHN ILSTLGLRPS VTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPNCEITFIE SEEGSGRGAA LVSAVACKKA CMLAQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion. In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. Required to provide D-glucose 6-phosphate for the synthesis of glycogen. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.
  • 基因功能参考文献：
    1. The enhancing effect of INGAP-PP on glucose-induced insulin release could be partly ascribed to its effect on glucokinase activity and glucose metabolism and is mainly mediated by the PI3K/AKT pathway. PMID: 25906449
    2. Arcuate nucleus glucokinase activation may represent a CNS mechanism that underlies the oft-described phenomena of the "sweet tooth" and carbohydrate craving. PMID: 25485685
    3. F0 maternal BPA exposure during gestation and lactation can induce impaired glucose homeostasis in the F2 offspring through DNA methylation change in Gck. PMID: 24793715
    4. Data indicate that iron facilitates diabetes-mediated elevation of oxidative/nitrative stress, simultaneously impairs liver glucokinase, and can be a link between enzymatic changes and hepatic dysfunction. PMID: 22884880
    5. The inhibitory effect of Urotensin IIon insulin secretion is associated with a suppression of GCK expression, not regulation of PDX-1 expression. PMID: 20401447
    6. Results indicate that Pax6 acts on the regulation of glucagon secretion at least through the transcriptional control of GCK, GPR40, and GIPR. PMID: 22778220
    7. Precedence of hypoglycemia diminishes glucokinase responses to recurring hypoglycemia in basal A2 noradrenergic neurons. PMID: 21488089
    8. Modulation of GK by a novel glucokinase activator (GKA) that may enable the design of new and improved GKAs. PMID: 22044397
    9. Glucokinase is localized in the nucleus and cytoplasm of glial tanycytes; its expression increases during postnatal development of the hypothalamic region. PMID: 20531973
    10. Data report that DNA hypermethylation is correlated with a decline in hepatic glucokinase (Gck) and L-type pyruvate kinase (LPK) expression in high-fat diet-induced obese rats as compared with the control diet group. PMID: 21239437
    11. investigation of mechanisms in regulation of glucokinase expression in pancreatic islets: roles of biotin, signal transduction pathways (involving soluble guanylate cyclase or protein kinase G), and insulin (autocrine function) PMID: 19560332
    12. Our findings indicate that both glucokinase and glucokinase regulatory proteins may be functionally active before birth and that they can act within a glucose sensor system involved in controlling food intake. PMID: 19807849
    13. To identify the binding site of these phosphate esters, we took advantage of the homology of GKRP to the isomerase domain of GlmS (glucosamine-6-phosphate synthase) and created 12 different mutants of rat GKRP. PMID: 11756407
    14. Activation of glucokinase gene expression by hepatic nuclear factor 4alpha in primary hepatocytes. PMID: 11950391
    15. results suggest that the IRS-1-PI 3-kinase pathway is essential for insulin-induced expression of SREBP-1c and glucokinase genes PMID: 12031952
    16. the likely mediator of glucosensing in both glucose-excited and glucose-inhibited central neurons PMID: 12086933
    17. Improved metabolic disorders of insulin receptor-deficient mice by transgenic overexpression of this enzyme in the liver. PMID: 12242462
    18. Substrate-induced nuclear export and peripheral compartmentalization in liver correlates with glycogen deposition PMID: 12369705
    19. glucokinase produces glucose-6-phosphate which has a role in activating hepatic glycogen synthase PMID: 12519761
    20. Protein kinase B has a role in insulin-dependent induction of glucokinase mRNA in hepatocytes. PMID: 14505487
    21. a modest hepatic overexpression of glucokinase is sufficient to alter expression of metabolic genes without changing the expression of SREBP-1c PMID: 14674713
    22. programming may influence methylation patterns in glucokinase at a distance from the promoter, or in genes encoding factors that regulate basal glucokinase expression PMID: 14706571
    23. PPAR-gamma can directly activate glucokinase expression in liver and may contribute to improving glucose homeostasis in type 2 diabetes. PMID: 14749268
    24. Hypothalamic glucokinase may be important component of glucoprivic counterregulatory responses. PMID: 15111491
    25. insulin-dependent hepatic glucokinase expression is regulated by SREBP-1c PMID: 15123649
    26. Glucokinase(GK) translocation from nucleus to cytoplasm in lean but not in Zucker diabetic fatty(ZDF) rats. Unresponsiveness of hepatic glucose to rise in plasma glucose and insulin in prediabetic ZDF rats associated with impaired GK translocation. PMID: 15138155
    27. These results indicate that glucose-stimulated insulin release is decreased by chronic exposure to tacrolimus due to reduced ATP production and glycolysis derived from reduced glucokinase activity. PMID: 15479952
    28. Glucokinase (GK), a key enzyme for pancreatic (beta)-cell glucose sensing, was found in both the embryonic and adult hypothalamus. PMID: 15707679
    29. Overexpression of PFK2 potentiated glucokinase expression in hepatocytes transduced with an adenoviral vector-encoding glucokinase. PMID: 15983194
    30. asparagine-leucine motifs of glucokinase located in the hinge region confer binding to glucokinase regulatory protein (GRP) PMID: 16186382
    31. The role of glucokinase as a critical regulator of neuronal glucosensing in ventromedical hypothalamic neurons was studied using a glucokinase activator and RNA interference. PMID: 16443775
    32. These results showed that increases in SREBP-1c were neither necessary nor sufficient for Glucokinase induction in hepatocytes, while at the same time they underscored the role of SREBP-1c as a key regulator of Fatty acid synthase. PMID: 16834571
    33. A defect is reported in glucose-induced dissociation of Gck from the regulatory protein in Zucker diabetic fatty rats in the early stages of diabetes. PMID: 17204595
    34. In weanlings maintained on an hig fat diet for the first, second, or third week of gestation, hyperglycemia, a reduction in beta-cell volume and glucokinase mRNA. PMID: 17414058
    35. coordinate roles for glucokinase and PFK2 in the elevated hepatic glycolysis in fa/fa Zucker rats PMID: 17553851
    36. Diurnal variation of glucokinase-mRNA in pancreas. PMID: 17712721
    37. Increases in intracellular glucose and net energy yield may be correlated with elevated GK and SUR1 gene transcription, respectively, in local glucose sensing neurons. PMID: 17889836
    38. Results describe the impact of estradiol on caudal fourth ventricular lactate infusion and hypoglycemia-associated MCT2, GLUT3, GLUT4, GCK, and sulfonylurea receptor-1 gene expression in the ovariectomized female rat lateral and ventromedial hypothalamus. PMID: 18084728
    39. mechanisms downstream of AMP-activated protein kinase activation, involving phosphorylation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and GKRP are involved in the ATP-independent inhibition of glucose-induced glucokinase translocation PMID: 18199594
    40. Ventromedial hypothalamic GK activity is an important regulator of the counterregulatory response to insulin-induced hypoglycemia. PMID: 18292346
    41. the epigenetic modification of the hepatic Gck promoter may represent an important marker for diabetogenic potential during the ageing process. PMID: 18496667
    42. gender-dimorphic dorsal vagal complex GCK gene responses occur after single and serial intermediate-acting insulin administration PMID: 18612599
    43. analysis of activation and translocation of glucokinase in rat primary hepatocytes PMID: 18812575
    44. Endogenous lipophilic molecules modulate hepatic Gck expression. PMID: 19173678
    45. A mechanism for LXRalpha in controlling the gene expression of LGK that involves activation through SREBP-1c and PPARgamma and inhibition through SHP, is proposed. PMID: 19366697
    46. Gestational high-fat programming impairs insulin release and reduces Gck immunoreactivity PMID: 19604517
    47. A study using inhibitors of PI 3-kinase and a conditionally active form of protein kinase B (cAKT) suggests that insulin induces the GCK gene in hepatocytes via the PI3-kinase/PKB signaling pathway PMID: 11042116

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Mitochondrion.
  • 蛋白家族：
    Hexokinase family
  • 组织特异性：
    Expression is restricted to the liver and pancreatic islets (at protein level).
  • 数据库链接：

    KEGG: rno:24385

    STRING: 10116.ENSRNOP00000019625

    UniGene: Rn.10447