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  4. Recombinant Rat Glucose-6-phosphate 1-dehydrogenase (G6pdx)

Recombinant Rat Glucose-6-phosphate 1-dehydrogenase (G6pdx)

  • 货号：
    CSB-YP356495RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356495RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP356495RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356495RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356495RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    G6pdx
  • Uniprot No.：
  • 别名：
    G6pdx; G6pdGlucose-6-phosphate 1-dehydrogenase; G6PD; EC 1.1.1.49
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-515
  • 氨基酸序列
    AEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPEDTFIVGY ARSRLTVDDI RKQSEPFFKV TPEERPKLEE FFARNSYVAG QYDDPASYKH LNSHMNALHQ GMQANRLFYL ALPPTVYEAV TKNIQEICMS QTGWNRIIVE KPFGRDLQSS NQLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTD SDDVRDEKVK VLKCISEVET DNVVLGQYVG NPSGEGEATN GYLDDPTVPH GSTTATFAAA VLYVENERWD GVPFILRCGK ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH KIDREKPQPI PYVYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cytosolic glucose-6-phosphate dehydrogenase that catalyzes the first and rate-limiting step of the oxidative branch within the pentose phosphate pathway/shunt, an alternative route to glycolysis for the dissimilation of carbohydrates and a major source of reducing power and metabolic intermediates for fatty acid and nucleic acid biosynthetic processes.
  • 基因功能参考文献：
    1. an investigation was carried out regarding the inhibitory effects caused by various metal ions (Fe(2+) , Pb(2+) , Cd(2+) , Ag(+) , and Zn(2+) ) on G6PD enzyme activities, as per Beutler method at 340 nm under in vitro conditions. Lineweaver-Burk diagrams were used for estimation of the IC50 and Ki values for the metals. Ki values for Pb(+2) , Cd(+2) , Ag(+) , and Zn(+2) were 113.3, 215.2, 19.4, and 474.7 muM, respectiv... PMID: 28557068
    2. 20-HETE elicited mitochondrial superoxide production and promoted secretory phenotype of vascular smooth muscle cells by activating MAPK1-Elk-1, all of which are blocked by inhibition of G6PD. PMID: 26921441
    3. Examination of changes in enzyme activities of erythrocyte glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in rats given Naringenin and Lead acetate. PMID: 25228019
    4. Data show that intake of the Livingstone potato (Plectranthus esculentus N.E.Br) feed by the diabetic rats resulted in significant amelioration of hepatic glucose-6-phosphophate dehydrogenase activity in comparison with the diabetic control. PMID: 25076344
    5. Glucose-6-phosphate dehydrogenase alleviated the signs of behavioural and biochemical effects of AlCl3-treatment suggesting its involvement in the pathogenesis of Aluminum neurotoxicity and its potential therapeutic benefit. PMID: 25109721
    6. Altered glucose metabolism and G6PD overactivation play a key role in switching the pulmonary artery smooth muscle cells from the contractile to synthetic phenotype. PMID: 25480333
    7. G6PD plays a key role in increasing hypoxia-induced CD133 positive stem cell survival in hypertensive lungs. PMID: 25063801
    8. upregulation of G6PD causes an increase in the accumulation of pentose and pentosidine, which might be associated with insulin resistance in the condition of obesity. PMID: 23015612
    9. Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver PMID: 23233666
    10. The enzymes of glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase,and glutathione reductase were purified from rat kidney in one chromatographic step consisting of the use of the 2',5'-ADP Sepharose 4B by using different elution buffers. PMID: 21930213
    11. These data suggest that up-regulation of G6PD in pancreatic beta-cells would induce beta-cell dysregulation through reactive oxygen species accumulation in the development of type 2 diabetes. PMID: 21248143
    12. G6PD activity in epithelium of SI and LI decreases with aging due to the accumulation of significant amounts of enzyme bound to cell organelles PMID: 12389736
    13. activation by peroxynitrite elicited a rapid stimulation of pentose phosphate pathway activity PMID: 12414804
    14. G6PD is the main source of NADPH for iNOS synthesis of nitric oxide following exposure of RINm5F insulinoma cells to IL-1 beta. PMID: 12475221
    15. In cardiomyocytes G6PD activity is increased by cellular oxidative stress with translocation of G6PD to the cell membrane Inhibition of G6PD depletes cytosolic GSH levels and results in cardiomyocyte contractile dysfunction through calcium dysregulation PMID: 12829617
    16. Protective role of glucose-6-phosphate dehydrogenase activity in the metabolic response of C6 rat glioma cells to polyunsaturated fatty acid exposure. PMID: 12838507
    17. Role for glucose-6-phosphate dehydrogenase in the regulation of inducible nitric oxide synthase gene expression in glial cells. PMID: 12930785
    18. Dependence of G6PD gene expression on cell hypoxixa and intracellular redox status and the differential hypoxic regulation of glucose-metabolizing enzymes. PMID: 15225644
    19. arachidonic acid inhibits the insulin stimulation of glucose-6-phosphate dehydrogenase mRNA accumulation by stimulating the p38 mitogen-activated protein kinase pathway, thereby inhibiting insulin signal transduction PMID: 16210322
    20. In oxygen-induced retinopathy, aldosterone reduced G6PD mRNA. A reduction in G6PD may be an early response to aldosterone. PMID: 19038868

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  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Glucose-6-phosphate dehydrogenase family
  • 数据库链接：

    KEGG: rno:24377

    STRING: 10116.ENSRNOP00000053157

    UniGene: Rn.11040