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  4. Recombinant Rat Glutamate decarboxylase 1 (Gad1)

Recombinant Rat Glutamate decarboxylase 1 (Gad1)

  • 货号：
    CSB-YP009159RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009159RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009159RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009159RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009159RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gad1
  • Uniprot No.：
  • 别名：
    Gad1; Gad67Glutamate decarboxylase 1; EC 4.1.1.15; 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-593
  • 氨基酸序列
    MASSTPSPAT SSNAGADPNT TNLRPTTYDT WCGVAHGCTR KLGLKICGFL QRTNSLEEKS RLVSAFRERQ ASKNLLSCEN SDPGARFRRT ETDFSNLFAQ DLLPAKNGEE QTVQFLLEVV DILLNYVRKT FDRSTKVLDF HHPHQLLEGM EGFNLELSDH PESLEQILVD CRDTLKYGVR TGHPRFFNQL STGLDIIGLA GEWLTSTANT NMFTYEIAPV FVLMEQITLK KMREIIGWSN KDGDGIFSPG GAISNMYSIM AARYKYFPEV KTKGMAAVPK LVLFTSEHSH YSIKKAGAAL GFGTDNVILI KCNERGKIIP ADLEAKILDA KQKGFVPLYV NATAGTTVYG AFDPIQEIAD ICEKYNLWLH VDAAWGGGLL MSRKHRHKLS GIERANSVTW NPHKMMGVLL QCSAILVKEK GILQGCNQMC AGYLFQPDKQ YDVSYDTGDK AIQCGRHVDI FKFWLMWKAK GTVGFENQIN KCLELAEYLY AKIKNREEFE MVFNGEPEHT NVCFWYIPQS LRGVPDSPER REKLHRVAPK IKALMMESGT TMVGYQPQGD KANFFRMVIS NPAATQSDID FLIEEIERLG QDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the production of GABA.
  • 基因功能参考文献：
    1. Prenatal caffeine exposure induced a high expression of hippocampal GAD67 in rats. PMID: 29111460
    2. Findings support the hypothesis that the epigenetic factors HDAC1 and DAXX are part of a complex network of genes that contribute to the synaptic regulation of GAD67 and GAD65 and help to promote the functional integrity of GABAergic interneurons located within stratum oriens-CA3/2 of the hippocampus PMID: 27733539
    3. No changes in either Arc or GAD protein expression were observed in primary auditory cortex in the immediate post-noise exposure period, confirming other reports that auditory cortical plasticity may not occur until later in the development of tinnitus. PMID: 27702572
    4. these findings indicate that modifying TLR4 gene expression in the periaqueductal gray stimulates expression of the downstream signaling molecule, GAD67, which decreases Glu levels and increases GABA levels. This mechanism may explain the inhibition of withdrawal syndrome in morphine-dependent rats. PMID: 28306133
    5. Results from this study indicate that social subordination is associated with several region-specific alterations in GAD67 mRNA expression in central stress circuits. PMID: 26066725
    6. The GAD65-independent mechanism for targeting of GAD67 to synaptic vesicles in neurons is not functional in islet beta-cells. PMID: 25647668
    7. study found that re-expression of GAD67 in neurons following stroke may play a role in aberrant cell cycle activation, consequently being pro-apoptotic PMID: 24983209
    8. experimental reduction of GAD65/67 expression bilaterally in the NAcbC is sufficient to increase impulsivity in LI rats. PMID: 23973096
    9. No changes in GAD67 and GAD65 mRNA expression between control and bile duct ligated rats PMID: 23904086
    10. NAP-22 could participate in the transport of GAD65 and GAD67 to the presynaptic termini and their retention on the synaptic vesicles as an anchoring protein. PMID: 23376695
    11. Serotonergic system/GAD67 neurons constitute a unique neuronal population with distinctive neurochemical and electrophysiological properties and high responsiveness to innocuous stressor. PMID: 23055511
    12. Elevated expression of GAD1 mRNA, but not of GAD2 mRNA, is found in the rat lateral striatum. PMID: 22332935
    13. GAD65 is found in mossy fiber terminals of Wistar and GAERS rat hippocampus. This enzyme does not show an increase in these terminals in absence epilepsy. PMID: 22194107
    14. results suggest that GAD65 and gad67 are cleaved after ubiquitination and degradation of an unknown binding partner by the proteasome. PMID: 20405034
    15. The above results suggested GAD67 gene expression was dynamically regulated by alternative promoters and splicing during postnatal rat testis maturation. PMID: 19911306
    16. GAD67 is inhibited following cell hypoxia via protein phosphorylation resulting in downregulation of GABA synthesis in cultured pheochromocytoma-12 cells. PMID: 20969567
    17. There is a maternal effect on the epigenetic regulation of GAD1 expression that is comparable with that observed for the glucocorticoid receptor gene. PMID: 20881131
    18. expression of GAD67 mRNA was increased in many forebrain regions, suggesting that long-term voluntary exercise enhances forebrain GABA synthesis capacity but in a region-specific manner PMID: 18801833
    19. Thus, depending on the region, NMDAR antagonism appears to influence expression of PV or GAD67, but not both. PMID: 19885653
    20. data demonstrate that expression of this GABAergic marker is highly regulated during development, with greatest levels found at an age when animals are transitioning out of the postnatal period. PMID: 19701789
    21. Functions to synthesize GABA; a demonstration of GABA synthesis and vesicular transport into synaptic vesicles. PMID: 12634427
    22. GAD67/Gad1 isoform is distributed in the reticular formation, the spinal trigeminal nucleus, and in neurons of the dorsomedial medulla. PMID: 14512139
    23. acute furosemide treatment reduced the expression of GAD67 mRNA, the active holoenzyme predictive of GABA synthesis, within the lamina terminalis PMID: 14596865
    24. in 6-hydroxydopamine-lesioned rats, GAD67 mRNA levels in striatonigral and striatopallidal pathways were selectively modified, and the modification correlated to dopamine agonist priming PMID: 14622157
    25. The GAD(67), but not GAD(65), is expressed in the mossy fibers of developing rats. In adults, GAD(67) is no longer detectable, unless seizures are induced. PMID: 14642440
    26. The mRNA expression of GAD65 is up-regulated in the vestibular nuclei bilaterally 50 h after labyrinthectomy. In the flocculus, GAD65 mRNA expression isbilaterally up-regulated 50 h post-operatively. PMID: 14663191
    27. palmitoylation of GAD65 regulates the trafficking of the protein from Golgi membranes to an endosomal trafficking pathway in axons that is dependent on Rab5a and is required for the targeting of several synaptic vesicle proteins to presynaptic clusters PMID: 15039456
    28. The effects of a subthalamic nucleus lesion on 6-OHDA- or repeated D2 antagonist-induced changes in globus pallidus GAD(67) mRNA expression in parvalbumin[PV]+ and PV- neurons was examined in rats PMID: 15044549
    29. Many areas of the brainstem and cerebellum involve double-labeled neurons with GLYT2 and GAD67 mRNAs and suggests that the corelease of glycine and GABA from single neurons is more widespread than has been reported. PMID: 15140559
    30. With age, overall expression of GAD(67) mRNA decreases in the area surrounding the organum vasculosum of the lamina terminalis and in the anteroventral periventricular nucleus. Young rats display a diurnal rhythm in GAD(67) mRNA in both regions. PMID: 15271064
    31. The results revealed a significant decline in GAD-IR cells between middle and old age in CA1 but not in dentate gyrus or CA3. PMID: 15368530
    32. estradiol repletion in ischemic rat brain selectively decreases GAD67 mRNA levels but does not alter steady-state GABA concentrations PMID: 16094313
    33. GAD67-ir cells were localized in regions known to contain high GABA content, including the ventrolateral medulla, raphe nuclei, and area postrema, but were absent from all motor nuclei, although dense terminal labeling was discerned in these regions. PMID: 16255028
    34. These data suggest that inhibition of GABA transmission in the NAc is related to METH behavioral sensitization, whereas activation of GABA transmission in the caudate is associated with METH-induced neurotoxicity. PMID: 17056007
    35. Data suggest that GABA synthesis by GAD67 may be elevated in middle-aged females in specific brain regions at critical times related to the LH surge, and that lack of changes in GABA levels in these regions may result in the attenuation of the surge. PMID: 17680886
    36. investigated the genomic responsiveness of neuroanatomically-defined populations of glutamate decarboxylase (GAD)-immunoreactive (-ir) neurons in this region of the brain to hindbrain glucoprivation PMID: 17934249
    37. The findings suggest that EA at St36 possess some curative effect on epileptic rats, related with change of GAD(67) mRNA level in DG region. PMID: 18022144
    38. post-transcriptional processing mechanisms, alternative splicing and polyadenlyation, may play a crucial role in regulating rat GAD67 gene expression PMID: 18758993
    39. Thus, downregulations of GlyRalpha1 and GAD67 may be involved both in the increased excitability observed in the CIC after unilateral deafness and consequently in the tinnitus frequently observed in unilateral adult deaf patients. PMID: 18973578
    40. Within the first three postnatal weeks the expression of both GAD65 and GAD67 mRNAs reached adult levels in hippocampus, cortex, and cerebellum. The olfactory bulb showed by far the highest expression of GAD65 as well as GAD67 transcripts. PMID: 19190758
    41. whereas somatic expression of AC3 and GAD67 may be non-overlapping, areas that exhibit punctate GAD67 (and are high in synaptic turnover) may be more vulnerable to MK801 exposure. PMID: 19596402

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  • 蛋白家族：
    Group II decarboxylase family
  • 数据库链接：

    KEGG: rno:24379

    STRING: 10116.ENSRNOP00000000008

    UniGene: Rn.91245