Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Growth factor receptor-bound protein 14 (Grb14)

Recombinant Rat Growth factor receptor-bound protein 14 (Grb14)

  • 货号：
    CSB-YP009888RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009888RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009888RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009888RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009888RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Grb14
  • Uniprot No.：
  • 别名：
    Grb14Growth factor receptor-bound protein 14; GRB14 adapter protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-538
  • 氨基酸序列
    TTSLQDGQS AAGRAGAQDS PLAVQVCRVA QGKGDAQDPA QVPGLHALSP ASDATRRGAM DRRKAKDLEV QETPSIPNPF PELCCSPLTS VLSAGLFPRS NSRKKQVIKV YSEDETSRAL EVPSDVTARD VCQLLILKNH YVDDNSWTLF EHLSHTGVER TVEDHELLTE VLSHWVMEED NKLYLRKNYA KYEFFKNPMY FFPEHMVSFA TEMNGDRSLT QIPQVFLSSN TYPEIHGFLH AKEQGKKSWK KAYFFLRRSG LYFSTKGTSK EPRHLQFFSE FSTSNVYMSL AGKKKHGAPT PYGFCFKPTK AGGPRDLKML CAEEDQSRMC WVTAIRLLKY GMQLYQNYMH PSQARSACSS QSVSPMRSVS ENSLVAMDFS GQKTRVIDNP TEALSVAVEE GLAWRKKGCL RLGNHGSPTA PSQSSAVNMA LHRSQPWFHH RISRDEAQQL ITRQGPVDGV FLVRDSQSNP RTFVLSMSHG QKIKHFQIIP VEDDGEVFHT LDDGHTKFTD LIQLVEFYQL NKGVLPCKLK HYCARMAV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
  • 基因功能参考文献：
    1. These results show that Chfr and Grb14 collaborate in a negative feedback loop controlling insulin-stimulated cell division. PMID: 25578860
    2. The phosphorylation status of the BPS region of Grb14 determines the positive or negative role it will play in insulin receptor signaling. PMID: 24350791
    3. Grb14 is a normal physiological modulator of CNG channel function in vivo. PMID: 20890309
    4. endogenous Grb14 exerts a negative feedback control on insulin receptor catalytic activity. This study thus demonstrates that Grb14 is a physiological regulator of liver insulin signaling PMID: 18657188
  • 亚细胞定位：
    Cytoplasm. Endosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    GRB7/10/14 family
  • 数据库链接：

    KEGG: rno:58844

    STRING: 10116.ENSRNOP00000046233

    UniGene: Rn.30028