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  4. Recombinant Rat Guanine nucleotide-binding protein G (s) subunit alpha isoforms short

Recombinant Rat Guanine nucleotide-binding protein G (s) subunit alpha isoforms short

  • 中文名称：
    大鼠Gnas重组蛋白
  • 货号：
    CSB-YP351815RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Gnas重组蛋白
  • 货号：
    CSB-EP351815RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Gnas重组蛋白
  • 货号：
    CSB-EP351815RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Gnas重组蛋白
  • 货号：
    CSB-BP351815RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Gnas重组蛋白
  • 货号：
    CSB-MP351815RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gnas; Gnas1Guanine nucleotide-binding protein G(s) subunit alpha isoforms short; Adenylate cyclase-stimulating G alpha protein; G-alpha-8
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-394
  • 氨基酸序列
    MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE SGKSTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK EAIETIVAAM SNLVPPVELA NPENQFRVDY ILSVMNVPNF DFPPEFYEHA KALWEDEGVR ACYERSNEYQ LIDCAQYFLD KIDVIKQADY VPSDQDLLRC RVLTSGIFET KFQVDKVNFH MFDVGGQRDE RRKWIQCFND VTAIIFVVAS SSYNMVIRED NQTNRLQEAL NLFKSIWNNR WLRTISVILF LNKQDLLAEK VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST ASGDGRHYCY PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ YELL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. Stimulates the Ras signaling pathway via RAPGEF2.
  • 基因功能参考文献：
    1. Our data revealed a positive correlation between hepatic Gsalpha-cAMP signal axis and fasting blood glucose (FBG) in slight insulin resistance stage of HIgh Sugar High Fat-diet rats and diabetic db/db mice. The current finding thus suggested hepatic Gsalpha-cAMP signal axis plays a central role in regulating of FBG during the developing and development of T2DM. PMID: 28260721
    2. Translocation of Galphas, which shows delayed onset after chronic antidepressant treatment of rats or of C6 glioma cells, tracks with the delayed onset of therapeutic action of antidepressants PMID: 27432886
    3. Activated Galpha s translocates from the plasma membrane and, through interaction with tubulin/microtubules in the cytosol, is important for neurite formation, development, and outgrowth. PMID: 25691569
    4. activated XLalphas and Galphas traffic differently, and this may form the basis for the differences in their cellular actions PMID: 21890629
    5. Data show that the interdomain opening is coupled to receptor binding via the C-terminal helix of G(alpha), the extension of which is a high-affinity receptor binding element. PMID: 21606326
    6. NESP55 has a role in a secreting vesicle pool found in the central and peripheral nervous system PMID: 12438142
    7. Palmitoylation ensures proper localization of G-protein Galpha(s) to the plasma membrane and facilitates bimolecular interactions of Galpha(s) with the beta-adrenergic receptor and adenylyl cyclase. PMID: 12614155
    8. expression of Galphas and Galphai protein subunits and adenylate cyclase in rat ovary, breast and pituitary are regulated by Luteinizing hormone-releasing hormone antagonist Cetrorelix PMID: 14767559
    9. Gs subunits and beta2 androgen receptors utilize different internalization mechanisms. PMID: 15297467
    10. PKA-mediated phosphorylation of the beta1-adrenergic receptor promotes Gs/Gi switching PMID: 15381255
    11. Data suggest that alpha(1)-adrenoceptor stimulation reduces the amplitude of the I(to) by activating a G(alphas) protein and the cAMP/PKA signaling cascade, which in turn leads to I(to) channel phosphorylation. PMID: 15496483
    12. a nucleotide-free state of Galphas is induced by Cu2+ and Zn2+ PMID: 15546818
    13. Absence of Gsalpha imprinting in human bone and fat. Osteodystrophy and obesity in pseudohypoparathyroidism(PHP) Ia and PPHP(pseudo-PHP) patients despite presence of normal Gsalpha allele likely due to Gsalpha haploinsufficiency in these tissues. PMID: 15579796
    14. data suggest that higher expression of somatostatin receptor subtype 1 and 2 mRNA induced by the gsp oncogene may be a mechanism by which gsp-positive tumors show a greater response to somatostatin PMID: 16379030
    15. in rat preglomerular microvessels activation of A2AR is coupled to EET release upstream of adenylyl cyclase activation and EETs stimulate mono-ADP-ribosyltransferase, resulting in Gsalpha protein activation PMID: 16478979
    16. Somatotroph adenomas in young patients seem to carry somatic Gs alpha mutations at a lower frequency, and germ-line or early postzygotic mutational events may be responsible for the shortened latency of tumorigenesis. PMID: 16631471
    17. The switch to Gs coupling by MOR in response to chronic morphine, attenuated by opioid antagonist cotreatment, leads to a two-pronged stimulation of adenylyl cyclase utilizing both Galpha and Gbetagamma subunits of the Gs protein novel to this receptor. PMID: 16967511
    18. The differential intracellular expression of NESP55-IR in spinal motoneurons and autonomic neurons suggests that NESP55 may be processed into different secretory granules and may be involved in both constitutive and regulated pathways in these neurons. PMID: 18067150
    19. Data show that NESP55 positive neurons in the rat superior cervical ganglion project to a number of targets including the submandibular gland, the cervical lymph nodes, the forehead skin, the iris, but not to the thyroid. PMID: 18539096
    20. Data demonstrated that activated Galpha(s) directly bound to tubulin and cellular microtubules, and fluorescence microscopy showed that cholera toxin-activated Galpha(s) colocalized with microtubules. PMID: 19237344

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor.
  • 蛋白家族：
    G-alpha family, G(s) subfamily
  • 组织特异性：
    Isoform Gnas-3 is abundant in brain with intermediate levels in skeletal muscle and very low levels in other tissues.
  • 数据库链接：

    UniGene: Rn.127731