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  4. Recombinant Rat Integrin-linked protein kinase (Ilk)

Recombinant Rat Integrin-linked protein kinase (Ilk)

  • 中文名称：
    大鼠Ilk重组蛋白
  • 货号：
    CSB-YP860792RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Ilk重组蛋白
  • 货号：
    CSB-EP860792RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Ilk重组蛋白
  • 货号：
    CSB-EP860792RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Ilk重组蛋白
  • 货号：
    CSB-BP860792RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Ilk重组蛋白
  • 货号：
    CSB-MP860792RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    IlkIntegrin-linked protein kinase; EC 2.7.11.1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-452
  • 氨基酸序列
    MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Regulates cell motility by forming a complex with PARVB. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • 基因功能参考文献：
    1. Investigated the role of ILK in cutaneous wound contraction. We found that ILK was involved in cutaneous wound healing, and ILK and PI3K/AKT inhibitors inhibited wound contraction and re-epithelialization, consequently delaying wound healing in vivo. PMID: 27111285
    2. high extracellular concentration of phosphate induced senescence in cultured smooth muscle through the activation of IGF-1 receptor and ILK overexpression PMID: 26467393
    3. These effects of digoxin were shown to be mediated, at least in part, through an increase in ILK/Akt signaling and a decrease in GSK-3beta signaling in PDGFBB-stimulated VSMCs PMID: 26311435
    4. Suggest a potential role for ILK, the cytoskeleton and ILK signalling partners including Rho in regulating intrahepatic sinusoidal endothelial cell eNOS expression and function. PMID: 24906011
    5. These observations demonstrate an important role for beta1-integrin/ILK signaling in regulating astrogliosis from ESCs and suggest ILK as a potential target for limiting glial scar formation after nervous system injury. PMID: 25098415
    6. the results provide evidence that HGF can accelerate reepithelialization in skin wound healing by dedifferentiation of epidermal cells in a manner related to the b1-integrin/ILK pathway. PMID: 24490163
    7. ILK mediates Rho-dependent functional effects in activated stellate cells, and raise the possibility that ILK is important in cross-talk with the G-protein-coupled receptor system PMID: 22064318
    8. Data suggest that understanding the ILK related mechanism of cardiac maladaptation leads to a new strategy for treatment of heart failure after infarction. PMID: 21949693
    9. ILK overexpression was the key event that involved mononuclear cells infiltration and vascular SMCs migration at early stage. PMID: 21350838
    10. Integrin-linked kinase activity increased rapidly 5 min after strain induction in both human Caco-2 and rat intestinal epithelial cell-6 (IEC-6)cells. PMID: 21084641
    11. Result suggest that ILK and PINCH contribute to stabilization of axonal and dendritic structures and the structural changes occurring in long-term diabetes. PMID: 20347724
    12. Integrin-linked kinase is a positive mediator of L6 myoblast differentiation PMID: 14550274
    13. Ilk is required for laminin-2-induced oligodendrocyte cell spreading and CNS myelination. PMID: 14581460
    14. ILK may be involved in the fibrotic or senescent process in the aging kidney. PMID: 15528771
    15. Ilk, PNCH1 and alpha-parvin form a complex to costameres in neonatal rat ventricular myocytes. This complex is stimulated by fibronectins and phenylephrine, and by both by drug synergism, to regulate cardiac myocyte hypertrophy. PMID: 16170337
    16. Direct inhibition of MLCP in the absence of Ca2+ unmasks ILK activity, which phosphorylates LC20 at Ser-19 and Thr-18 to induce contraction; ILK is probably the kinase responsible for myosin diphosphorylation in vascular smooth muscle cells and tissues. PMID: 16201970
    17. ILK plays an important pathophysiological role in vivo in liver wound healing PMID: 16728409
    18. ILK is involved in the phosphorylation of ERK 1/2, p38 MAPK, JNK, and PKB and that selective inhibition of ILK expression by siRNA results in a significant decrease in their phosphorylation. PMID: 16941698
    19. identify ILK as a novel target in vascular smooth muscle cells for anti-atherosclerotic therapy PMID: 16962068
    20. Expression of integrin-linked kinase (ILK) was significantly increased in ELMO1 overexpressing cells. PMID: 17021600
    21. The integrin-linked kinase (ILK)- glycogen synthase kinase-3beta (GSK-3beta) pathway plays a key role in regulating dendrite formation in developing mammalian neurons. PMID: 17182785
    22. Our results show that ILK and cell-matrix adhesion proteins play an important role in the process of matrix-induced hepatocyte differentiation. PMID: 17194454
    23. ILK has a key role in the formation of neuronal polarity PMID: 17490631
    24. Regulation of cell-matrix contacts and beta-catenin signaling in vascular smooth muscle by integrin-linked kinase: implications for intimal thickening. PMID: 18080083
    25. ILK functions to maintain smooth muscle cell quiescence in the normal artery. PMID: 18535176
    26. RAAV-delivered Tet-On-regulated siRNA expression can conditionally regulate gene expression in rat brain. Inhibition of ILK expression has no effect on apoptosis and locomotor and stereotypical behaviors, but decreases dendritic density. PMID: 18602949
    27. ILK is involved in cocaine sensitization PMID: 18702665
    28. mRNA expression levels of both integrin beta1 and ILK were up regulated after mechanical loading (P<0.05), meanwhile, it was higher and peaked faster in 3D cultures than in the 2D cultures. PMID: 19215949
    29. the ILK gene transduction of Mesenchymal stem cells (MSCs) further assisted cell survival and adhesion, and improved myocardial damage when compared with MSC only after transplantation. PMID: 19489098

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  • 亚细胞定位：
    Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cytoplasm, myofibril, sarcomere.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: rno:170922

    STRING: 10116.ENSRNOP00000025906

    UniGene: Rn.95042