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  4. Recombinant Rat Islet amyloid polypeptide (Iapp)

Recombinant Rat Islet amyloid polypeptide (Iapp)

  • 货号：
    CSB-YP010931RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010931RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010931RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010931RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010931RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    IappIslet amyloid polypeptide; Amylin; Diabetes-associated peptide; DAP
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    38-74
  • 氨基酸序列
    KCN TATCATQRLA NFLVRSSNNL GPVLPPTNVG SNTY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
  • 基因功能参考文献：
    1. IAPP role in the pancreatic islet cell damage in the Type 2 diabetes mellitus. PMID: 29523142
    2. this study reinforces the notion that IAPP amyloidogenesis is governed by precise intermolecular interactions involving specific Asn side chains. PMID: 28665109
    3. C4BP protects beta-cells from IAPP cytotoxicity by modulating IAPP fibril formation extracellularly and also, after uptake by the cells, by enhancing cholesterol synthesis. PMID: 27566545
    4. ventromedial hypothalamus amylin signaling is required for full leptin signaling and protection from high fat diet induced obesity PMID: 26676252
    5. Secondary Structure of Rat and Human Amylin PMID: 26221949
    6. cross-seeding assemblies between hIAPP and rIAPP oligomers PMID: 25706385
    7. Inhibition of IAPP aggregation by insulin depends on the insulin oligomeric state regulated by zinc ion concentration PMID: 25649462
    8. The data indicate minimal membrane disruption by both human and rat-IAPP20-29 when compared to the full-length IAPP peptide. PMID: 23493863
    9. Amylin receptors are located mostly on presynaptic glutamatergic terminals connecting to area postrema neurons not displaying hyperpolarization-activated cation current. Amylin concentrations can increase glutamate release enough to cause cell firing. PMID: 23219578
    10. C4BP binds to IAPP thereby limiting complement activation and may be enhancing formation of IAPP fibrils from cytotoxic oligomers PMID: 22334700
    11. co-expression of calcitonin receptor(a) with rRAMP1 or 3 produces functional AMY receptor subtypes in rats, which display equivalent potency for rAmy and raCGRP PMID: 22014233
    12. at 8 h after mixing, rat amylin blocks the N-terminal beta-sheet of fibrils. At 24 h after mixing, rat amylin blocks neither beta-sheet and forms its own beta-sheet PMID: 22522254
    13. Data show that the multiplicity in islet amyloid polypeptide (rIAPP) assembly structures indicates the polydispersity of the rIAPP(8-37) beta-like motifs. PMID: 22106265
    14. The results implicate amylin in the control of maternal adaptations, possibly exerting its actions on maternal behaviors via amylin receptors present in brain regions to which preoptic neurons project. PMID: 21965599
    15. hIAPP overexpression inhibits insulin and IAPP secretion in response to glucose affecting the activity of K(ATP) channels PMID: 21984830
    16. an actin-mediated and cholesterol-dependent mechanism for selective uptake and clearance of amylin oligomers, impairment of which greatly potentiates amylin toxicity. PMID: 21865171
    17. The study is comparing the structural properties of human and rat islet amyloid polypeptide by MD computer simulations at 310 and 330 K and shows the effect of the disulfide bridge on the conformation of Iapp. PMID: 21266296
    18. Data show that IAPP oligomeric species that arise through stochastic nucleation on membranes and result in disruption of the lipid bilayer. PMID: 21606325
    19. Ca2+ entry via L-type Ca2+ channels, activation of PKC and Ca2+ release from sarcoplasmic reticulum through ryanodine-sensitive Ca2+ channels may be involved in amylin's positive inotropic effect. PMID: 21138812
    20. Rats centrally infused with amylin had lower body weight gain controls independent of whether prior body weight was decreased by fasting, increased by voluntary overfeeding or unmanipulated. PMID: 20416330
    21. Data indicate that basal insulin, leptin, ghrelin, and amylin do not encode AT mass in rats dynamically regulating BW and adiposity during recovery from OW. PMID: 20668029
    22. we present detailed solution structures of rat amylin; the similarity of rat and human amylin sequences near the N-terminus suggests that human amylin might also exhibit a similar a-helical secondary structure PMID: 20141758
    23. This His/Arg-18 mutation results in reduced affinity binding of human IAPP to insulin in comparison to rat IAPP. PMID: 19146426
    24. Inhibition of human IAPP amyloid formation by rat IAPP can be rationalized by a model that postulates formation of an early helical intermediate during amyloid formation where the helical region is localized to the N-terminal region of IAPP. PMID: 20028124
    25. these newly observed differences between human and rat amylin in solution and their possible relation to aggregation and to the physiological function of amylin binding to the calcitonin receptor PMID: 19948124
    26. Data show that the three specific proline residues in human and rat amylin play a dominant negative role in fibril formation. PMID: 12589759
    27. conformational preferences of hIAPP(20-29) in membrane-mimicking environments PMID: 12717720
    28. Feeding-induced amylin release activates area postrema neurons projecting to relay stations known to transmit meal-related signals to the forebrain. Activation of this pathway seems to coincide with an inhibition of lateral hypothalamic area neurons. PMID: 12958059
    29. amylin has a role in evoking a JNK1-mediated apoptotic pathway, which is partially dependent and partially independent of caspase-8, and in which caspase-3 acts as a common downstream effector PMID: 14532296
    30. These results indicate that amylin is positively regulated by cyclic AMP and protein kinase A through the transcription factors HNF-1 and NFY. PMID: 14615061
    31. results support the hypothesis that endogenous amylin plays an essential role in reducing meal size and increasing the postmeal interval of satiety PMID: 15130879
    32. found that amylin increased GSK3alpha activity, which in turn led to increased phosphorylation of glycogen synthase and decreased glycogen synthesis de novo in rat soleus muscle PMID: 15572200
    33. findings are consistent with the hypothesis that membrane-active pre-fibrillar IAPP oligomers insert into beta cell membranes in NIDDM PMID: 15710403
    34. analysis of pro-islet amyloid polypeptide processing in the constitutive and regulated secretory pathways of beta cells PMID: 15802374
    35. conditions that promote weakly stable alpha-helical conformations may promote IAPP aggregation PMID: 16142909
    36. Data show that GABAA receptor antagonists prevent abnormalities in leptin, insulin and amylin actions on paraventricular hypothalamic neurons of overweight rats. PMID: 16553787
    37. The data suggests that both KNT and KNTvd4(-) participate in microtubule-dependent secretion of amylin in islet beta-cells. PMID: 16890462
    38. The inability of IAPP to self assemble can be ascribed, in part, to several slowly exchanging conformations evident as multiple chemical shift assignments in the immediate vicinity of three proline residues residing outside of this helical region. PMID: 17123962
    39. Amylin did not influence energy expenditure or respiratory quotient in normal or food-restricted rats. PMID: 17428511
    40. Results indicate that the lipostatic signals leptin and insulin may synergize with amylin to reduce food intake. PMID: 17481674
    41. amylin effectively reduced food intake in ad libitum chow fed rats despite the low level of amylin-induced c-Fos expression in the area postrema PMID: 17703089
    42. Central and peripheral administration of amylin induces energy expenditure in anesthetized rats. PMID: 18346817
    43. amylin agonism restores leptin responsiveness in diet-induced obesity PMID: 18458326
    44. Rat amylin does not share neurotoxic properties with Abeta42. PMID: 18486611
    45. Amylin/leptin interact synergistically to reduce body weight and adiposity in diet-induced obese rodents through a number of anorexigenic and metabolic effects. PMID: 18669592
    46. NMR structures of the human and rat IAPP(1-19) peptides in DPC micelles PMID: 18989932
    47. the high-resolution structure of rat IAPP in the membrane-mimicking detergent micelles composed of dodecylphosphocholine was solved. PMID: 19456151
    48. Amylin is expressed in rat placenta and regulated by nutritional status. PMID: 19554505
    49. This studt indicted that the expression of amylin in the mammalian brain and described its distribution in the preoptic area of the hypothalamus. Our data also indicate that the level of amylin mRNA is dramatically induced in mother rats PMID: 19811608

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 组织特异性：
    Abundant in the islets of Langerhans but is not present in the brain or seven other tissues examined.
  • 数据库链接：

    KEGG: rno:24476

    STRING: 10116.ENSRNOP00000016614

    UniGene: Rn.11394