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  4. Recombinant Rat Legumain (Lgmn)

Recombinant Rat Legumain (Lgmn)

  • 货号：
    CSB-YP012903RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012903RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012903RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012903RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012903RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Lgmn; Prsc1Legumain; EC 3.4.22.34; Asparaginyl endopeptidase; Protease; cysteine 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    18-325
  • 氨基酸序列
    VHI GVDDPEDGGK HWVVIVAGSN GWYNYRHQAD ACHAYQIIHR NGIPDEQIIV MMYDDIANNE ENPTPGVVIN RPNGTDVYKG VPKDYTGEDV TPENFLAVLR GDEEAVKGKG SGKVLKSGPR DHVFVYFTDH GATGILVFPN EDLHVKDLNK TIRYMYEHKM YQKMVFYIEA CESGSMMNHL PDDIDVYATT AANPNESSYA CYYDEERSTY LGDWYSVNWM EDSDVEDLTK ETLHKQYHLV KSHTNTSHVM QYGNKSISTM KVMQFQGMKH RASSPISLPP VTHLDLTPSP DVPLTILKRK LLRTN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
  • 基因功能参考文献：
    1. Full-length (45 kDa) endogenous BHMT was found to be cleaved in an autophagy-dependent (3-methyladenine-sensitive) manner in isolated rat hepatocytes to generate a novel N-terminal 10-kDa fragment (p10), at the cleavage site of legumain. PMID: 21610319
    2. Observed is an increase of legumain expression in the peri-infarct area of rats after transient occlusion of the middle cerebral artery for 120 mins with a maximum expression at 24 and 48 h. PMID: 20234379
  • 亚细胞定位：
    Lysosome.
  • 蛋白家族：
    Peptidase C13 family
  • 组织特异性：
    Detected in kidney cortex (at protein level).
  • 数据库链接：

    KEGG: rno:63865

    STRING: 10116.ENSRNOP00000010101

    UniGene: Rn.206021