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  4. Recombinant Rat Matrix metalloproteinase-9 (Mmp9)

Recombinant Rat Matrix metalloproteinase-9 (Mmp9)

  • 货号：
    CSB-YP014679RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP014679RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP014679RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP014679RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP014679RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mmp9; Matrix metalloproteinase-9; MMP-9; EC 3.4.24.35; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    108-708
  • 氨基酸序列
    FQT FDGDLKWHHH NITYWIQSYT EDLPRDVIDD SFARAFAVWS AVTPLTFTRV YGLEADIVIQ FGVAEHGDGY PFDGKDGLLA HAFPPGPGIQ GDAHFDDDEL WSLGKGAVVP TYFGNANGAP CHFPFTFEGR SYLSCTTDGR NDGKPWCGTT ADYDTDRKYG FCPSENLYTE HGNGDGKPCV FPFIFEGHSY SACTTKGRSD GYRWCATTAN YDQDKADGFC PTRADVTVTG GNSAGEMCVF PFVFLGKQYS TCTSEGRSDG RLWCATTSNF DADKKWGFCP DQGYSLFLVA AHEFGHALGL DHSSVPEALM YPMYHYHEDS PLHEDDIKGI HHLYGRGSKP DPRPPATTAA EPQPTAPPTM CSTAPPMAYP TGGPTVAPTG APSPGPTGPP TAGPSEAPTE SSTPDDNPCN VDVFDAIADI QGALHFFKDG RYWKFSNHGG NQLQGPFLIA RTWPAFPSKL NSAFEDPQPK KIFFFLWAQM WVYTGQSVLG PRSLDKLGLG SEVTLVTGLL PRRGGKALLI SRERIWKFDL KSQKVDPQSV TRLDNEFSGV PWNSHNVFQY QDKAYFCHDK YFWRVSFHNR VNQVDHVAYV TYDLLQCP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 基因功能参考文献：
    1. Diacerein reduces joint damage and hypersensitivity associated with inflammation induced by complete Freund's adjuvant through the inhibition of astroglial activation and decreases the expression of TRPV1 and MMP-9 in the rat spinal cord. PMID: 26481104
    2. Tubular Expression and Urinary Excretion of MMP-9 Protein Were Increased in Association with Albumin Overload in the Diabetic Kidneys. PMID: 28805677
    3. In conclusion, these results suggest that TEN exerts an anti-inflammatory effect by down-regulating the release of NO, MMP-9 and cytokines. PMID: 28392358
    4. Ang II is involved in the degradation of myosin light chain kinase in pressure overload-induced cardiac hypertrophy and that this process was mediated by MMP9. PMID: 29262413
    5. Jmjd3 mRNA depletion inhibited Mmp-3 and Mmp-9 gene expressions and significantly attenuated blood-spinal cord barrier permeability and the loss of tight junction proteins PMID: 27425890
    6. High MMP9 expression is associated with seizures. PMID: 28501723
    7. no significant relationship between plasma MMP-9 activity or aortic MMP-9 mRNA levels and the percentage of incomplete elastin lamellae or the aortic wall thickness PMID: 26547079
    8. High MMP9 expression is associated with uraemic vascular calcifications. PMID: 26333546
    9. that mesenchymal stem cells protect against obstruction-induced renal fibrosis, in part, by decreasing STAT3 activation and STAT3-dependent MMP-9 production PMID: 27760767
    10. absent in heterotopic ossification PMID: 26919547
    11. Results indicate that N-acetyl-cysteine (NAC) can effectively alleviate remifentanil-induced postoperative hyperalgesia (RIH) via powerfully inhibiting matrix metalloproteinase-9 (MMP-9)activation in dorsal root ganglia (DRGs). PMID: 28199982
    12. High mmp9 expression is associated with Retinitis Pigmentosa. PMID: 27893855
    13. Data suggest that increased blood pressure and decreased pup weight in ischemic model of pregnancy-induced hypertension are associated with decrease in Mmp9 homodimer and increase in Mmp9/Timp1 complex in placenta, uterus, and uterine artery; thus Mmp9 and Timp1 appear to be involved in uteroplacental/vascular remodeling in preeclampsia. (Mmp9 = matrix metalloproteinase 9; Timp1 = tissue inhibitor of metalloproteinase-1) PMID: 28506758
    14. PEX1 activation of the MMP-9 promoter triggers the pro-fibrotic response directed by PEX1. PMID: 27826738
    15. the up-regulation of MMP genes is mediated through degradation of class IIa histone deacetylases (HDACs) by certain cysteine cathepsins (Cts). PMID: 28157489
    16. Active fraction of tubers from Pueraria tuberosa (PTY-2)significantly attenuated the severity of diabetic nephropathy (DN) by increasing the expression and activity of matrix metalloproteinase-9 (Mmp-9). PMID: 26924185
    17. Therefore, the current study confirmed that MMP9 is important for the enhancement of the contractility of cerebrovascular smooth muscle cells in an in vitro rat model of subarachnoid hemorrhage PMID: 27633189
    18. Naringin has anti-fatigue effects which may be attributed to its property in modulating energy metabolism and reducing serum MMP-9 concentration. PMID: 27937032
    19. Alskiren attenuates signs of pulmonary fibrosis by decreasing pulmonary overexpression of MMP-9 and transforming growth factor beta1. PMID: 27154762
    20. Data suggest that, as non-alcoholic fatty liver disease progresses, expression of miR-21 (mirn21 microRNA), Mmp-9 (matrix metalloproteinase 9), and Timp-1 (tissue inhibitor of metalloproteinase-1) is up-regulated in liver, peaking when liver fibrosis is first detected; miR-21 has potential as a plasma biomarker for hepatic fibrosis. PMID: 27320964
    21. Atorvastatin reduced MMP9 levels in aging rat kidneys. PMID: 26680767
    22. Data suggests a mechanistic involvement of MMP-9 in aggravating hypertension-induced cerebrovascular pathologies. PMID: 26800984
    23. Our findings indicate that MMP9 may play an important role via inducing cell apoptosis in the development of diabetic retinopathy PMID: 26617754
    24. Increased circulating MMP-9 activity is associated with resistant albuminuria and a deleterious oxidative stress environment appears to be the underlying mechanism. PMID: 26733721
    25. Recombinant human BNP attenuates trauma/haemorrhagic shock-induced acute lung injury by inhibiting oxidative stress and PMID: 26852688
    26. This study demonstrated that stretching activated MMP-9 gene expressions and increased the proliferation of connective tissue but did not avoid muscle fiber atrophy or induce sarcomerogenesis of denervated rat soleus muscle PMID: 25960249
    27. MMP-9 expression was significantly increased in the gingiva in a model of gingival overgrowth. PMID: 25652432
    28. This study has shown a potential association of matrix metalloproteinase 9 with injury of the basilar artery and can be associated with early-stage brain injury following subarachnoid hemorrhage. PMID: 25627354
    29. Selective allosteric inhibition of MMP9 is efficacious in preclinical models of ulcerative colitis and colorectal cancer. PMID: 25961845
    30. In the presence of acute kidney injury in severe, acute pancreatitis, the renal expression of MMP-9 and VASP is related to damage of endothelial cells in capillaries, which reached a maximum at 24 h and declined afterwards. PMID: 26264698
    31. intestinal microbes with the capacity to produce collagenases and to activate host metalloproteinase MMP9 may break down collagen in the intestinal tissue contributing to anastomotic leak PMID: 25947163
    32. Data show that Ash1l and EZH2 methyltransferases protein expression was significantly elevated in fibrotic liver and decreased in mutant matrix metalloproteinase-9 (mMMP-9) and human hepatocyte growth factor (hHGF) gene transfer treated fibrotic livers. PMID: 25380300
    33. MMP-9 has a role in stress-induced downregulation of nectin-3 in hippocampal CA1 and associated behavioral alterations PMID: 25232752
    34. Up-regulation of reactive oxygen species-dependent matrix metalloproteinase-9 was found from high-glucose-challenged astrocytes PMID: 24395134
    35. The data indicated that early exercise significantly inhibited the ischemia-induced reduction of occludin, and an increase in MMP-9 promoted TIMP-1 expression. PMID: 23708107
    36. Inspiratory resistive breathing induces MMP-9 and -12 in the lung of previously healthy rats. PMID: 25595645
    37. Long term obesity creates environment promoting proliferative disorders in the prostate, leading to diffuse hyperplasia, via MMP9/VEGF pathway. PMID: 25529509
    38. Cold exposure-induced MMP-9 via activation of RAS might play a critical role in the initiation of cold exposure-induced stroke during chronic hypertension and doxycycline shows protective effects against cold exposure-induced stroke. PMID: 23800500
    39. Explored expression of MMP-9 and MDM2 in the oncogenesis of lung cancer in rats. PMID: 25063292
    40. Transient increases in brain MMP-9 levels are seen during cue-induced cocaine relapse. PMID: 25326689
    41. MMP-9 as a potential biomarker for osteosarcoma PMID: 24704305
    42. Curcumin attenuated hepatic ischemia/reperfusion-induced combined restrictive and obstructive lung disease by reducing lung inflammation and MMP-9 activity. PMID: 24815146
    43. In a rat mammary neoplasm model matrix metalloproteinase 9 had consistent localization throughout cancer cells, fibroblast and stroma. PMID: 25343559
    44. The location of Mmp9 and activity can be detected by means of in situ zymography and confocal microcopy, and this methodology may provide a useful tool in studies on the role of gelatinases in tooth development, maturation and in pathological conditions. PMID: 22897943
    45. MMP-9 induction is required for brain astrocyte migration. PMID: 24018979
    46. This study demonistrated that mmp9 throughout hippocampus at all ages but expression levels were highest during the first postnatal week. PMID: 24114974
    47. CCL19/CCR7 contributes to the migration of bone marrow mesenchymal stem cells by upregulating MMP9 potentially via the PI3K/Akt pathway. PMID: 25086360
    48. Matrix metalloproteinase-9, which is known to be activated by oxidative stress and S-nitrosylation of proteins, was suppressed more extensively by puerarin than losartan in diabetic rats. PMID: 24454919
    49. Fish oil treatment resulted in less severe hindlimb deficits after TBI as assessed with the beam walk test, decreased cerebral IgG infiltration, and decreased TBI-induced expression of the Mmp9 gene one day after injury. PMID: 24342130
    50. an overall inhibitory effect of curcumin on Gelatinase B in norepinephrine-induced hypertrophic stress in H9c2 cardiomyocytes which may contribute in the prevention of extracellular matrix remodeling. PMID: 24116115

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    KEGG: rno:81687

    STRING: 10116.ENSRNOP00000023965

    UniGene: Rn.10209