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  4. Recombinant Rat Merlin (Nf2)

Recombinant Rat Merlin (Nf2)

  • 货号：
    CSB-YP726923RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP726923RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP726923RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP726923RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP726923RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nf2Merlin; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomin; Fragment
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-586
  • 氨基酸序列
    IASRMSFSSL KRKQPKTFTV RIVTMDAEME FNCEMKWKGK DLFDLVCRTL GLRETWFFGL QYTIKDTVAW LKMDKKVLDH DVSKEEPVTF HFLAKFYPEN AEEELVQEIT QHLFFLQVKK QILDEKVYCP PEASVLLASY AVQAKYGDYD PSVHKRGFLA QEELLPKRVI NLYQMTPEMW EERITAWYAE HRGRARDEAE MEYLKIAQDL EMYGVNYFTI RNKKGTELLL GVDALGLHIY DPENRLTPKI SFPWNEIRNI SYSDKEFTIK PLDKKIDVFK FNSSKLRVNK LILQLCIGNH DLFMRRRKAD SLEVQQMKAQ AREEKARKQM ERQRLAREKQ MREEAERSRD EPERRVLHMK EEATMANEAL MRSEETADLL AEKAQITEEE AKLLAQKAAE AEQEMQRIKA TAIRTEEEKR LMEQKVLEAE VLALKMAEES ERRAKEADQL KQDLQEAREA ERRAKQKLLE IATKPTYPPM NPIPAPLPPD IPSFDIIGDS LSFDFKDTDM KRLSMEIEKE KVEYMEKSKH LQEQLNELKT EIEALKLKER ETALDILHSE HSDSGTSSKH NTIKKPQAQG RRPICI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.
  • 基因功能参考文献：
    1. beta-AR stimulation affects post-translational modifications of NF2 via the involvement beta1-AR/PKA/cAMP pathway, and NF2 plays a pro-apoptotic role in beta-AR-stimulated myocyte apoptosis via the phosphorylation (inactivation) of YAP and involvement of mitochondrial death pathway PMID: 29709009
    2. Results demonstrate that Schwannomin plays an essential role in inducing and/or maintaining the SC's spindle shape, and by stabilizing the bipolar morphology, Sch promotes the alignment of SCs with axons and ultimately influences myelin segment length. PMID: 21182951
    3. Paxillin binds schwannomin and regulates its density-dependent localization and effect on cell morphology PMID: 12118253
    4. c-merlin has a role as a negative regulator of both muscle growth and motility PMID: 14991710
    5. PIKE-L is an important mediator of merlin growth suppression PMID: 15598747
    6. Widespread expression of merlin in brain and its association with protein kinase A suggest a role for merlin in brain biology. PMID: 15797715
    7. The data support the significance of regulated Rac signaling in mediating Schwann cell-axon interaction and suggest that controlling Rac activity as a possible therapy for schwannomas. PMID: 16571745
    8. MYPT-1-PP1delta and its substrate merlin are part of a previously undescribed tumour suppressor cascade that can be hindered in two ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI-17 PMID: 16885985
    9. Study demonstrates that Akt-mediated merlin phosphorylation regulates the function of merlin in the absence of an inactivating mutation. PMID: 17891137

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cytoplasm, cytoskeleton. Nucleus.
  • 数据库链接：

    KEGG: rno:25744

    STRING: 10116.ENSRNOP00000010690

    UniGene: Rn.46695