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  4. Recombinant Rat Mesencephalic astrocyte-derived neurotrophic factor (Manf)

Recombinant Rat Mesencephalic astrocyte-derived neurotrophic factor (Manf)

  • 货号：
    CSB-YP002118RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002118RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002118RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002118RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002118RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Manf
  • Uniprot No.：
  • 别名：
    Manf; ArmetMesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-179
  • 氨基酸序列
    LRPGDCEVC ISYLGRFYQD LKDRDVTFSP ATIEEELIKF CREARGKENR LCYYIGATDD AATKIINEVS KPLAHHIPVE KICEKLKKKD SQICELKYDK QIDLSTVDLK KLRVKELKKI LDDWGEMCKG CAEKSDYIRK INELMPKYAP KAASARTDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions.
  • 基因功能参考文献：
    1. MANF is developmentally regulated and may play a role in the maturation of the central nervous system. PMID: 24587361
    2. The results of this study indicated that MANF expression was upregulated in the activated glial cells, which may contribute to the mechanism of ischemia-induced neural injury. PMID: 23173607
    3. MANF binding to the plasma membrane also required the RTDL sequence and was inhibited with a peptide known to interact with KDELRs, suggesting MANF binds KDELRs at the surface. PMID: 23255601
    4. sarcoplasmic/endoplasmic reticulum calcium depletion triggered MANF secretion by decreasing its retention PMID: 22637475
    5. MANF and C-MANF protect neurons intracellularly as efficiently as Ku70. PMID: 21047780
    6. These results suggest that cerebral ischemia-induced ARMET expression may be protective to the neurons PMID: 19773801

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  • 亚细胞定位：
    Secreted. Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen.
  • 蛋白家族：
    ARMET family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000019107

    UniGene: Rn.229163