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  4. Recombinant Rat Monocarboxylate transporter 1 (Slc16a1), partial

Recombinant Rat Monocarboxylate transporter 1 (Slc16a1), partial

  • 中文名称：
    大鼠Slc16a1重组蛋白
  • 货号：
    CSB-YP021403RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Slc16a1重组蛋白
  • 货号：
    CSB-EP021403RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Slc16a1重组蛋白
  • 货号：
    CSB-EP021403RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Slc16a1重组蛋白
  • 货号：
    CSB-BP021403RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Slc16a1重组蛋白
  • 货号：
    CSB-MP021403RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Slc16a1
  • Uniprot No.：
  • 别名：
    Slc16a1; Mct1; Monocarboxylate transporter 1; MCT 1; Solute carrier family 16 member 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.
  • 基因功能参考文献：
    1. 7ACC2 is an inhibitor of mitochondrial pyruvate transport; the blockade of pyruvate import into mitochondria prevents extracellular lactate uptake as efficiently as a MCT1 inhibitor PMID: 29572438
    2. Following cerebral ischemia, monocarboxylate transporter 1 (MCT1) expression was upregulated in the peri-infarct striatum but not in the cortex of the brain. In vitro ischemia models were used to induce oligodendrocyte stress as well. An increase in MCT1 expression was detected in oligodendrocyte precursor cells after a mild oxygen-glucose deprivation. PMID: 29205833
    3. The MCT1 mRNA and membrane protein expression varied with estrous cycle stage, with OVX females having higher expression than males, indicating that female sex hormones may play a role in MCT1 regulation. PMID: 29249221
    4. Our results are proposing that in the developing brain white matter, MCT1 is predominately expressed in oligodendrocyte though it mainly expressed in astrocyte in early postnatal, which indicate that MCT1 may involve in the oligodendrocyte development and myelination. PMID: 27982679
    5. Decreased expression of MCT1/4 in distal airway epithelium may disrupt lung branching morphogenesis and thus contribute to the development of pulmonary hypoplasia in the nitrofen-induced congenital diaphragmatic hernia model. PMID: 26996590
    6. The transport mechanism of 4-PBA from blood to brain across the BBB likely involves MCT1. PMID: 27026010
    7. The Canonical Wnt/beta-Catenin Pathway Increased Expression of MCT1 on the Plasma Membrane of Rat Brain Endothelial Cells. PMID: 26872974
    8. MPC inhibition is the most sensitive anti-tumour target for LND, with additional inhibitory effects on MCT-mediated L-lactic acid efflux and glutamine/glutamate oxidation. PMID: 26831515
    9. results suggest that the EA treatments activated lactate metabolism in the resident astrocytes around the ischemic area and up-regulated the expression of MCT1 in these astrocytes which facilitated the transfer of intracellular lactate PMID: 26037401
    10. The metabolic Km for L-lactate closely resembled that for MCT-mediated hepatic uptake in vitro PMID: 24854892
    11. After a 2-h exercise session, MCT1 increased in the cortex and hippocampus 5h postexercise. PMID: 24610532
    12. Cyclic AMP signaling reduces the functionality of Mct1 in cerebrovascular endothelial cells. PMID: 24454947
    13. Results suggest that acute exercise-induced increases in MCT1 and MCT4 mRNA expression may be possibly mediated by AMPK activation, at least in part in fast-twitch muscle. PMID: 23886299
    14. These data indicate that MCT1 is the high affinity lactate transporter and MCT4 is the high affinity butyrate transporter in the intestinal epithelial cell line IEC-18. PMID: 23344966
    15. we show for the first time the regulated and unregulated vesicular trafficking of Mct1 in cerebrovascular endothelial cells; processes which have significance for better understanding normal brain energy metabolism PMID: 22925948
    16. MCT1 was lost on microvessels and upregulated on astrocytes in the hippocampal formation in all models of temporal lobe epilepsy. PMID: 21856423
    17. MCT1 is increased at all ages, occurring 5-10-fold more in adult than in immature rats with status epilepticus. PMID: 21624469
    18. Transfer of nicotinate from blood to retina across the inner blood-retinal barrier occurs primarily via H(+)-coupled monocarboxylate transporters. PMID: 21741392
    19. MCT1 expression was doubled (P
    20. Administration of Phellinus linteus increased endurance exercise performance through inhibition of serotonin production in the brain and increased the expression of MCT1 and MCT4 in muscles. PMID: 21512297
    21. tanycytes participate in glucose sensing based on a metabolic interaction with neurons of the arcuate nucleus, which are stimulated by lactate released from MCT1 and MCT4-expressing tanycytes. PMID: 21297988
    22. we characterized the effects of intracellular pH in controlling MCT1 function PMID: 21192921
    23. Disrupting the expression of the astrocytic lactate transporters monocarboxylate transporter 4 (MCT4) or MCT1 causes amnesia, which, like long-term potentiation impairment, is rescued by L-lactate but not equicaloric glucose. PMID: 21376239
    24. MCT1 and MCT2 are inhibited by AR-C155858, which binds to an intracellular site involving transmembrane helices 7-10 PMID: 19929853
    25. Changes in MCT 1, MCT 4, and LDH expression are tissue specific in rats after long-term hypobaric hypoxia. PMID: 11896024
    26. Muscle contraction increases lactate transport while reducing sarcolemmal MCT4, but not MCT1. PMID: 11934671
    27. These studies have shown that 1) with STZ-induced diabetes, MCT1 (Slc16a1) and MCT4 (Slc16a4) are reduced in skeletal muscle and/or the heart and 2) exercise training alleviated these diabetes-induced reductions. PMID: 12611763
    28. T3 thyroid hormone regulates MCT4, but not MCT1, protein expression and increases lactate transport rates. PMID: 12900382
    29. The loop between helix 4 and helix 5 in MCT1 is important for transport activity. PMID: 12946269
    30. Expression of the monocarboxylate transporter MCT1 in the rat brain between embryonic day 16 and postnatal day 14 produced MCT1-like immunoreactivity throughout the cortical anlage, particularly in the hippocampal anlage next to the ventricle. PMID: 12966567
    31. monocarboxylic acid transporter 1 and 2 levels in the adult brain implies a major role for these proteins to transfer glycolytic intermediates during cerebral energy metabolism PMID: 14534079
    32. A close correlation was found between local MCT1 and local GLUT1 densities. As local GLUT1 densities reflect local glucose metabolism in the brain, we conclude that local MCT1 densities are adjusted to local glucose metabolism and transport. PMID: 14729246
    33. MCT1 and MCT4 belong to a class of selected metabolic genes that are very rapidly up-regulated with an exercise stimulus. PMID: 15388779
    34. This T-cell-mediated regulation of MCT1 might contribute to a compensatory or protective mechanism in order to guarantee substrate pools for neurons and oligodendrocytes under inflammatory conditions. PMID: 15390096
    35. inhibition of MCT1 and MCT4 activity by pCMBS is mediated through its binding to CD147, acting as an ancillary protein required to maintain the catalytic activity of MCTs 1 and 4, as well as for their translocation to the plasma membrane PMID: 15917240
    36. MCT1 and COX are co-localised at both interfibrillar and subsarcolemmal cell domains in skeletal muscle PMID: 15932892
    37. carbonic anhydrase enhances MCT1 transport activity, independent of its enzymatic reaction center, presumably by binding to MCT1 PMID: 16174776
    38. monocarboxylic acid transporter-1 kinetic function is modulated by the cAMP signaling pathway in rat brain endothelial c PMID: 16301311
    39. Western blots showed that cytochrome oxidase (COX), NADH dehydrogenase, LDH, MCT1, and CD147 are abundant in mitochondrial fractions of L6 cells. Interactions among COX, MCT1, and CD147 in mitochondria were confirmed by immunoblotting. PMID: 16434551
    40. Our findings support the hypothesis that the expression of monocarboxylate transporter 1 and monocarboxylate transporter 4 in excitable tissues is regulated by activity. PMID: 16446038
    41. In conclusion, MCT1 expression was up-regulated in hepatocarcinoma cells and the IGF-IR signaling appeared to be coupled with the modulation of MCT1 expression. PMID: 16516162
    42. In conclusion, these studies have shown that testosterone induces an increase in both MCT1 and MCT4 proteins and their plasmalemmal content in skeletal muscle. PMID: 16959859
    43. We propose that TM3 of MCT1 lies alongside the TM of basigin with Arg86 adjacent to Glu218 of basigin. PMID: 17127621
    44. The correlation between MCT1 and NBC expression suggests that lactate transport may be facilitated by NBC in oxidative skeletal muscle, which may in turn favor better muscle pH regulation. PMID: 17609257
    45. Monocarboxylate transporter (MCT)-1 is up-regulated by PPARalpha. PMID: 18375207
    46. MCT1 belongs to the family of metabolic genes whose expression is regulated by PGC-1alpha in skeletal muscle. PMID: 18523157
    47. MCT1 transporters were up-regulated during chronic hyperglycemia at the level of brain substructures and independently of local cerebral glucose utilization PMID: 19118535
    48. Dietary pectin up-regulates monocaboxylate transporter 1 in the rat gastrointestinal tract. PMID: 19168540
    49. Increased MCT1 mRNA expression was detected in the injured cortex and hippocampus of animals with focal ischemia PMID: 19401710
    50. It is concluded that MCT1 transporters were upregulated during chronic nicotine infusion at the level of brain substructures and, at least partially, independently of LCGU. PMID: 19433117

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Monocarboxylate porter (TC 2.A.1.13) family
  • 组织特异性：
    Detected in erythrocytes (at protein level). Detected in brain, heart, kidney, lung, muscle, jejunum enterocytes and brain capillaries.
  • 数据库链接：

    KEGG: rno:25027

    STRING: 10116.ENSRNOP00000027234

    UniGene: Rn.6085