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  4. Recombinant Rat Mothers against decapentaplegic homolog 3 (Smad3)

Recombinant Rat Mothers against decapentaplegic homolog 3 (Smad3)

  • 货号：
    CSB-YP021788RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021788RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021788RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021788RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021788RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Smad3; Madh3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; SMAD family member 3; SMAD 3; Smad3
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-425
  • 氨基酸序列
    SSILPFTPP IVKRLLGWKK GEQNGQEEKW CEKAVKSLVK KLKKTGQLDE LEKAITTQNV NTKCITIPRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELRAMELCE FAFNMKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEIPAEF PPLDDYSHSI PENTNFPAGI EPQSNIPETP PPGYLSEDGE TSDHQMNHSM DAGSPNLSPN PMSPAHNNLD LQPVTYCEPA FWCSISYYEL NQRVGETFHA SQPSMTVDGF TDPSNSERFC LGLLSNVNRN AAVELTRRHI GRGVRLYYIG GEVFAECLSD SAIFVQSPNC NQRYGWHPAT VCKIPPGCNL KIFNNQEFAA LLAQSVNQGF EAVYQLTRMC TIRMSFVKGW GAEYRRQTVT STPCWIELHL NGPLQWLDKV LTQMGSPSIR CSSVS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • 基因功能参考文献：
    1. The expression of TGF-beta and p-smad3 declined after anoctamin-1 (ANO1) over-expression in cardiac fiborblasts. PMID: 28539652
    2. Formononetin significantly protected against balloon injuryinduced neointima formation in the carotid artery of a rat model; this effect may be associated with the regulation of VSMC proliferation and migration through altered TGFbeta1/Smad3 signaling. PMID: 30066831
    3. experimental results show that Smad3 and TGF-beta1 play a protective role against ischemic stroke, as demonstrated by the reduced infarct size. PMID: 29704604
    4. In pancreata of mice and rats, TGFB promotes peripheral nociceptive sensitization via a direct effect on primary sensory neurons mediated by intra-neuronal SMAD3. PMID: 29505748
    5. cigarette smoke-induced upregulation of TGF-b1/Smad pathway components, including TGF-b1, TGF-bR1, phospho-Smad2, and phospho-Smad3, was also inhibited by ginsenoside Rg1. Additionally, ginsenoside Rg1 mimicked the effect of SB525334, a TGF-betaR1-Smad2/3 inhibitor, on suppression of EMT in CSE-induced HBE cells PMID: 29104873
    6. Scoparoene suppressed the phosphorylation level of Smad3 in TGF-beta1-stimialted HSC-T6 cells. PMID: 28623783
    7. miR-203 may inhibit HSC proliferation and suppress the expression of collagen-related genes. Furthermore, SMAD3 was identified as a direct target gene of miR-203. PMID: 28586069
    8. The results provide the first evidence that upregulation of TGFb/Smad3 in injured arteries induces local smooth muscle cells CXCR4 expression and cell migration, and consequently intimal hyperplasia. PMID: 27340942
    9. Collectively, edaravone may attenuate ARDS associated early pulmonary fibrosis through amelioration of oxidative stress and TGF-beta1/Smad3 signaling pathway. Edaravone may be a promising drug candidate for the treatment of ARDS-related pulmonary fibrosis in early period. PMID: 29102631
    10. small-interfering RNA (siRNA) silencing of Smad3 or Nox4 significantly suppressed TGF-b1-mediated CTGF expression in vascular smooth muscle cells (VSMC). Furthermore, Nox4 silencing or inhibition eliminated TGF-b1-induced Smad3 activation and interaction between Nox4 and Smad3 PMID: 26945889
    11. activation by cyclosporine A in gingival fibroblasts promotes cell proliferation, collagen and alpha-smooth muscle actin expressions PMID: 26738448
    12. Our results demonstrate that SARA/Smad3 pathway contributes to mechanism of seizure PMID: 28035691
    13. Phosphorylated nuclear levels of Smad3 were increased in hepatic stellate cells in response to brefeldin A treatment. Inhibition of Smad3 phosphorylation by SIS3 prevented the enhancement of collagen type I levels caused by BFA. Pretreatment with IRE1alpha inhibitors also prevented the increased p-Smad3 accumulation in the nucleus. An IRE1alpha-p38 MAPK-Smad pathway may be responsible for the fibrogenic action of BFA o... PMID: 27155082
    14. TGF-beta1-induced early Smad3 phosphorylation is independent of transient EGFR transactivation and ERK1/2 activation initiated by HB-EGF release, whereas Src-mediated chronic EGFR transactivation and ERK1/2 activation participate in Smad3 activation in a relatively modest and delayed manner. PMID: 28943431
    15. Both the in vitro and in vivo results clearly showed that, morin by acting on Hippo/Yap and TGF-beta1/Smad pathways, ameliorated experimental liver fibrosis, indicating that morin has potential for effective treatment of liver fibrosis. PMID: 28552397
    16. the expression of Runx2, ALK5 and Smad3 were influenced by SB431542 treatment. In conclusion, this preliminary study indicated that fluoride regulated osteoblastic TGFb1 signaling in bone turnover and cells differentiation via ALK5. PMID: 28125630
    17. BRG1 is a key mediator of the SMAD3-dependent regulation of cellular and behavioral plasticity that mediates cocaine seeking after a period of withdrawal. PMID: 27422367
    18. Substance P can promote hepatic stem cell proliferation and induce HSC activation via the TGF-beta1/Smad3 signaling pathway. PMID: 28647476
    19. Data show that caffeic acid phenethyl ester (CAPE) repressed the TGF-beta1/Smad3 signaling pathway in liver fibrosis. PMID: 28193525
    20. PTH pretreatment prevented TGF-beta1 and high glucose-induced Smad2/3 phosphorylation and consequent upregulation of fibronectin and type IV collagen within 4 h. PMID: 27530924
    21. Gln inhibits HG + H/R induced activation of the TGF-beta1-Smad3 pathway and decreases cell apoptosis in cardiomyocytes. PMID: 26946943
    22. Astragalus saponins and glycyrrhizic acid synergistically alleviated hepatic fibrosis via TGF-beta1/Smads signaling pathway inhibition in hepatic stellate cells. PMID: 27282665
    23. Qinggan Huoxue Recipe inhibits epithelial mesenchymal transformation in alcoholic liver fibrosis rats by modulating the TGF-beta1/Smad3 signaling pathway. PMID: 27217701
    24. the novel turtle shell decoction treatment significantly decreased the transforming growth factor beta 1 (TGF-b1) and Smad3 gene expression and increased inhibitory Smad7 gene expression in liver tissues of HF rats, suggesting that NTSD inhibited the ECM expression of HSC by downregulating the TGF-b1/Smad signaling pathway PMID: 27633729
    25. Suggest that epithelial mesencymal transformation up-regulation mediated by the TGF-beta/Smad3 pathway plays a role in postnatal pulmonary dysfunction associated with maternal exposure to PM2.5. PMID: 28041981
    26. silencing eIF3a might alleviate TGF-beta1-induced cardiac fibrogenesis in cardiac fibroblasts by inhibiting Smad3 activation. PMID: 27453280
    27. Bergenin (BEG) markedly reduced transforming growth factor-beta1 (TGF-beta1) production, down-regulated p-Smad2/3 expression and promoted Smad7 expression both in vivo and in vitro. In conclusion, BEG exerts the effective protective role against kidney injuries of diabetic rat PMID: 26954391
    28. CTRP3 attenuates post-infarct cardiac fibrosis by targeting Smad3 activation and inhibiting myofibroblast differentiation PMID: 26138247
    29. SAM had a protective effect on hepatic fibrosis in rats induced by CCl(4) combined with ethanol and the down-regulation of activity and expression of Smad3 were involved in the potential mechanisms. PMID: 27239849
    30. Smad3, a key downstream signal to TGFBeta1, is involved in pericontusional region after traumatic brain injury. PMID: 26253398
    31. Smad3 was a direct and functional target of miR-23b in heat-denatured fibroblasts. PMID: 26153982
    32. Data show that Smad3 protein transcriptional activation of E3 ubiquitin Llgase FBXO32 under stress is p21-activated kinase 1 Pak1 dependent in neonatal cardiomyocytes. PMID: 26483344
    33. Findings support a novel role for AMPK/TGF-beta/Smad3 pathway in controlling vascular smooth muscle cell growth. PMID: 26276823
    34. Epigallocatechin-3-gallate may be helpful for reducing the degree of TGF-b1- induced epithelial mesenchymal transdifferentiation of tubular epithelial cells which may be related to the signal transduction pathway of ERK, Smad3, and beta-catenin. PMID: 25867402
    35. Smad2 and Smad3 proteins recruit CBP1 to become transcriptional complexes. PMID: 25416030
    36. TGFbeta1 induced expression of p-Smad2/3 in cardiac fibroblasts (CFs) in a time-dependent manner, consistent with the role of TGFbeta1 in the promotion of fibronectin expression, suggesting the presence of the TGFbeta1/Smad signaling pathway in CFs PMID: 25538336
    37. For the first time, the fact that ROSI inhibits SMAD2 and SMAD3 signaling in a depot-specific manner in diabetic rats was demonstrated. PMID: 24500776
    38. C3aRA ameliorated T2DN by inhibiting IKBalpha phosphorylation and cytokine release, and also TGF-beta/Smad3 signaling and ECM deposition PMID: 25422985
    39. TGF-beta/Smad3 activated multiple genes related to development or cell differentiation. It down regulated transcription of SMC contractile genes as well as protein production of smooth muscle alpha actin, calponin, and smooth muscle myosin heavy chain. PMID: 24718260
    40. TGF-beta Stimulation of vascular smooth muscle cell led to the formation of a complex of Smad3 and HIF-1alpha that in turn activated the VEGF-A. PMID: 25010983
    41. IGFBPrP1 has a role in inducing liver fibrosis by inducing hepatic stellate cell activation and hepatocyte apoptosis via Smad2/3 signaling PMID: 24914373
    42. these results identify Sirt1 as an important protective factor for renal fibrosis in a CKD rodent model, and the protective function of Sirt1 is attributable to its action on TGF-beta/Smad3 signaling PMID: 24356887
    43. the hippocampus, ischemia-induced activation of Smad2 and Smad3 is associated with the response to stress but is not related to neuronal survival or death. PMID: 24290497
    44. Inhibition of ROS-induced Smad3 activation. PMID: 24086569
    45. Data suggest that Smad3 overexpression in granulosa cells promotes estradiol secretion and cell proliferation while inhibiting apoptosis; silencing of Smad3 expression reduces estradiol secretion and cell proliferation and increases apoptosis. PMID: 23690627
    46. study suggests that UII could induce collagen synthesis and secretion through upregulation of TGF-beta1 expression and secretion in VSMCs, and that TGF-beta1/Smad2/3 signaling might be one of the important pathways by which UII is involved in vascular fibrosis PMID: 23403244
    47. These data indicate that the phyto-power dietary supplement may inhibit the TGF-beta1/Smad signaling and relieve liver damage in experimental fibrosis. PMID: 23291610
    48. Smad3 protein gene expression was positively correlated with renal interstitial fibrosis progression. PMID: 23176567
    49. Maxacalcitol ameliorates tubulointerstitial fibrosis in obstructed kidneys by recruiting PPM1A/VDR complex to pSmad3. This recruitment promoted the dephosphorylation of pSmad3 and attenuated the pSmad3-dependent production of TGF-beta1. PMID: 22926646
    50. Data indicate that dimethylfumarate and Ad-Nrf2 repressed TGF-beta-stimulated Smad3 activity by inhibiting Smad3 phosphorylation, which was restored by siRNA-mediated knockdown of Nrf2 expression. PMID: 23056222

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Dwarfin/SMAD family
  • 组织特异性：
    Highly expressed in the brain and ovary. Detected in the pyramidal cells of the hippocampus, granule cells of the dentate gyrus, granular cells of the cerebral cortex and the granulosa cells of the ovary.
  • 数据库链接：

    KEGG: rno:25631

    STRING: 10116.ENSRNOP00000037346

    UniGene: Rn.10636