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  4. Recombinant Rat Mothers against decapentaplegic homolog 7 (Smad7)

Recombinant Rat Mothers against decapentaplegic homolog 7 (Smad7)

  • 货号：
    CSB-YP021793RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021793RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021793RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021793RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021793RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Smad7; Madh7; Mothers against decapentaplegic homolog 7; MAD homolog 7; Mothers against DPP homolog 7; SMAD family member 7; SMAD 7; Smad7
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-426
  • 氨基酸序列
    MFRTKRSALV RRLWRSRAPG GEDEEEGVGG GGGGGGLRGE GATDGRAYGA GGGGAGRAGC CLGKAVRGAK GHHHPHPPSS GAGAAGGAEA DLKALTHSVL KKLKERQLEL LLQAVESRGG TRTACLLLPG RLDCRLGPGA PASAQPAQPP SSYSLPLLLC KVFRWPDLRH SSEVKRLCCC ESYGKINPEL VCCNPHHLSR LCELESPPPP YSRYPMDFLK PTADCPDAVP SSDETGGTNY LAPGGLSDSQ LLLEPGDRSH WCVVAYWEEK TRVGRLYCVQ EPSLDIFYDL PQGNGFCLGQ LNSDNKSQLV QKVRSKIGCG IQLTREVDGV WVYNRSSYPI FIKSATLDNP DSRTLLVHKV FPGFSIKAFD YEKAYSLQRP NDHEFMQQPW TGFTVQISFV KGWGQCYTRQ FISSCPCWLE VIFNSR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • 基因功能参考文献：
    1. Human umbilical cord mesenchymal stem cells exosomes may promote Smad7 expression by inhibiting miR125b5p to increase myocardial repair in the rat acute myocardial infarction model. PMID: 29484378
    2. The results demonstrated that miRNA-195 activated hepatic stellate cells (HSCs) by targeting Smad7. PMID: 28929107
    3. the present findings indicated that miR-15a inhibition protects cardiomyocytes against H/R-induced apoptosis by upregulating the expression of its target, SMAD7, and downregulating the NF-kappaB p65 subunit. PMID: 28440490
    4. miR-21 blocks norepinephrine-mediated rat granulosa cell apoptosis by targeting SMAD7. PMID: 28473352
    5. Smad7 influenced NF-kappaB expression by regulating reactive oxygen species generation, and induced glomerular mesangial cell apoptosis to counter the Ang II-promoted proliferation. PMID: 28988741
    6. Cell internal signal transduction, miR-187 regulating Smad7 expression, plays a vital role in retinal ganglion cell survival. PMID: 26463627
    7. Data suggest that post-surgical heterotopic ossification (HO) may be prevented in vivo by local delivery of mothers against decapentaplegic homolog 7 (SMAD7). PMID: 26807862
    8. Smad-7 was highly expressed in the degenerative discs. PMID: 25811233
    9. MicroRNA-92a inhibition attenuates hypoxia/reoxygenation-induced cardiomyocyte apoptosis by up-regulating Smad7 expression. PMID: 24941323
    10. miR-17-5p promotes hepatic stellate cell proliferation and activation, at least in part via reduction of Smad7 PMID: 25915722
    11. Study found that STRAP and Smad7 are significantly decreased in the pilocarpine-induced epileptic rats compared with the normal rats PMID: 24577865
    12. findings suggest that canonical TGFbeta signaling induces suture closure at least in part through activation of FGF and ERK signaling and that Smad7 plays an important role in maintaining suture patency PMID: 23959527
    13. Smad7 gene transfer inhibits fibrogenic responses of cornea in rats after photorefractive keratectomy PMID: 23595375
    14. Overexpression of Smad7 suppressed ROS/MMP9-dependent collagen synthesis through regulation of heme oxygenase-1. PMID: 23661026
    15. silencing the expression of the DNMT1 gene ameliorated the suppression of Smad7 expression by TGF-beta1 PMID: 24211420
    16. In the early stage of diabetic nephropathy, increase in BMP-7 and Smad6/7 expression may play a role in the feedback regulation and restrain the development of diabetic nephropathy. PMID: 17437042
    17. the ectopic expression of Smad7 suppresses the dI1 and dI3 neural fates and concomitantly increases the number of dI4-dI6 spinal neurons. PMID: 21718693
    18. Enalapril increased the expression of Smad7 in renal interstitial fibrosis in rats. PMID: 19197123
    19. Transforming growth factor beta 1 (TGF-beta1)inhibits expression of Smad7 during activation of pancreatic stellate cells. PMID: 20943464
    20. Letter: Report decreased expression of Smad7 in chronic pancreatitis. PMID: 20182310
    21. data imply that decreased inhibitory Smad 7 signal in cardiac fibroblasts may play a role in the pathogenesis of cardiac fibrosis in the postmyocardial infarct heart PMID: 11959632
    22. TGF-beta-induced apoptosis in mesangial cells is mediated through the activation of caspase-3 by Smad7, but not by Smad2 or Smad3. PMID: 12234288
    23. role in post-translational regulation of Muc4 via the transforming growth factor-beta and interferon-gamma pathways in rat mammary epithelial cells PMID: 12668667
    24. Smad7 has a role in renal fibrosis in association with hypertension PMID: 15743788
    25. YB-1 activation via the Jak1 signaling pathway is required and sufficient to confer interferon-gamma-dependent activation of the smad7 gene. PMID: 16278212
    26. Expressions of HGF and Smad7 were increased significantly in the kidneys of diabetic rats. PMID: 16297324
    27. Epigallocatechin gallate enhanced the expression of Smad7. PMID: 17541159
    28. Potassium supplementation can reduce renal inflammation and hence, could modulate the progression of kidney injury in chronic kidney disease through upregulation of renal Smad7. PMID: 17634402
    29. Smad7 overexpression suppresses peritoneal inflammation induced by peritoneal dialysis fluid. PMID: 17704451
    30. Blockade of the TGF-beta/Smad signaling pathway may represent a novel therapeutic approach to prevent peritoneal fibrosis in patients treated with peritoneal dialysis. PMID: 17855642
    31. induction of Smad7 by HGF serves to limit acquisition of the myofibroblast phenotype in alveolar epithelial cells PMID: 18988920

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Dwarfin/SMAD family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: rno:81516

    STRING: 10116.ENSRNOP00000024831

    UniGene: Rn.29980