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  4. Recombinant Rat Natriuretic peptides B (Nppb)

Recombinant Rat Natriuretic peptides B (Nppb)

  • 货号：
    CSB-YP016021RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016021RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016021RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016021RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016021RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    NppbNatriuretic peptides B; Gamma-brain natriuretic peptide; Iso-ANP) [Cleaved into: Brain natriuretic peptide 45; 5 kDa cardiac natriuretic peptide; BNP-34)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    27-121
  • 氨基酸序列
    HPLG SPSQSPEQST MQKLLELIRE KSEEMAQRQL SKDQGPTKEL LKRVLRSQDS AFRIQERLRN SKMAHSSSCF GQKIDRIGAV SRLGCDGLRL F
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis.
  • 基因功能参考文献：
    1. Data show that approximately 60% of natriuretic peptide precursor type B (Nppb)-/- females developed mesenteric polyarteritis-nodosa (PAN)-like vasculitis in their life span, some as early as 4 months of age. PMID: 27162120
    2. NT-proBNP and BNP are potential early biomarkers for 2-DG-induced cardiac toxicity that can be useful to monitor 2-DG therapy in clinical trials. PMID: 26838190
    3. Cardioprotective effects of rutin via alteration in TNF-alpha, CRP, and BNP levels coupled with antioxidant effect in STZ-induced diabetic rats. PMID: 27443845
    4. Despite preserved LV structure and function, treatment with GYY increased the levels of the natriuretic peptides ANP and BNP in association with enhanced cyclic GMP levels, paralleled by higher cGMP-dependent protein kinase type I (cGKI) protein levels PMID: 26254181
    5. This is due to the fact that ANP is the main hormone of the natriuretic peptide system involved in the regulation of blood pressure in normal conditions, while BNP is the principal regulator of pressure in cardiovascular pathology PMID: 27228659
    6. Serum NT-proBNP levels significantly increased after 5 hours of the blunt chest trauma. PMID: 26261563
    7. Akt dependent signaling is involved in mediating the cardioprotection afforded by intermittent BNP infusion at early reperfusion, and may also participate in regulation of reperfusion induced BNP expression. PMID: 25698234
    8. These results suggest that proBNP is processed into BNP intracellularly by furin PMID: 26136529
    9. daily rhythm in heart expression diminished under rotating light-dark regimen conditions PMID: 25669688
    10. BNP knockout Dahl salt-sensitive rats demonstrated adult-onset hypertension. Systemic BNP overexpression reversed this, demonstrating a critical role of BNP defect in the development of systemic hypertension and associated end-organ damage in adulthood. PMID: 26063669
    11. BMP antagonist Noggin was able to inhibit stretch and hypertrophic agonist induced BNP and ANP expression. PMID: 25218476
    12. hsTnT and NT-proBNP strongly associated with incident heart failure among a diverse cohort of individuals with mild to severe chronic kidney disease PMID: 25278510
    13. Studied effect of atorvastatin on serum oxidative stress and N-terminal brain natriuretic peptide expression in rats. PMID: 25063069
    14. These preliminary data give new insights into the relationship between natriuretic peptide signaling and sKATP in the myocardium. PMID: 24477916
    15. The data suggest that the natriuretic peptides may perform several biological actions on sensory neurons via their binding to natriuretic peptide receptor B(NPR-B) in the dorsal root ganglion (DRG). PMID: 23454171
    16. BNP and adiponectin play important roles in cardiovascular homeostasis regulation. We investigated BNP and adiponectin serum levels followed by isoproterenol administration in a model of myocardial damage and explored the relationship between them. PMID: 24289757
    17. In an animal model of prediabetes, left ventricular hypertrophy is already present and BNP is the best early marker. PMID: 23497124
    18. BNP and NPR-A are expressed in the dorsal horn ganglion, and expressions are elevated in rats with chronic nonbacterial prostatitis. PMID: 22474982
    19. Data show taht hyperaldosteronism worsens hypertension-induced fibrosis through 2 inhibition of antifibrotic factors (B-type natriuretic peptide and bone morphogenetic protein 4). PMID: 22547442
    20. acute myocardial ischemia can directly upregulate BNP expression at the translational and transcriptional levels PMID: 21951964
    21. Elderly had significantly higher NT-proBNP levels. PMID: 18032399
    22. The measurement of circulating NT-proBNP concentration may be useful to screen for the presence of macro- and/or microvascular complications in type 2 diabetes. PMID: 16357805
    23. found a close correlation between pulmonary embolism degree and gene-expression of ANP, and BNP in the cardiac chambers with a selective increase in the right chambers of the heart PMID: 20559433
    24. There was significant increase in BNP mRNA expression 3hr after acute myocardial ischemia. PMID: 18404982
    25. Regulation of BNP-45 gene expression occurred at transcription as well as post-transcription level. Systemic chronic hypoxia could result in heart failure, especially when the hypoxia is severe and prolonged. PMID: 19752486
    26. The beneficial effect of BNP in cultured cardiomyocytes subjected to reperfusion is associated with attenuation of opening of mitochondrial permeability transition pores, resultant mitochondrial dysfunction and apoptosis. PMID: 19721230
    27. Mechanical overload in combined models of hypertension determines the evolution of hypertrophy and synthesis and secretion of ANP and BNP. PMID: 20139323
    28. Ischemia/reperfusion is responsible for increased BNP mRNA myocardial content independent of changes of ventricular cavity diastolic volume. PMID: 20108079
    29. Distinct roles of mitogen-activated protein kinase pathways in GATA-4 transcription factor-mediated regulation of B-type natriuretic peptide gene. PMID: 11827958
    30. BNP secretion was increased 6-fold, BNP mRNA levels 3-fold, and BNP promoter activity 3-5-fold following T(3) treatment, which was accompanied by an increase in myocyte size, sarcomeric organization, and protein synthesis. PMID: 12562779
    31. Thus BNP, which increases cyclic GMP independently of NO, is an important approach to prevent growth in the diabetic myocardium, where endothelium-dependent mechanisms are compromised. PMID: 12941780
    32. cardiac brain natriuretic peptide is upregulated at the transcriptional and translational levels by pro-inflammatory cytokines PMID: 15081310
    33. Load-induced activation of BNP gene expression is independent of calcineurin, protein kinase C anad protein phosphatase 2A. PMID: 15837527
    34. discussion of molecular regulation of the brain natriuretic peptide gene [review] PMID: 15911064
    35. Rat BNP transfected into diabetic transgenic mice prevents the progression of diabetic nephropathy. PMID: 16917760
    36. Calcium-dependent secretion and gene expression of A- and B-type natriuretic peptides are similarly regulated by calmodulin kinase II-dependent mechanisms. PMID: 17332063
    37. in response to myocyte stretching, mRNA levels of the mechanosensitive K(+) channel TREK-1, Brain Natriuretic Peptide and Endothelin-1 receptor B were significantly greater in sub-endocardial myocardium than sub-epicardial myocardium. PMID: 17345093
    38. NPR-B is present in cultured rat GABAergic ACs, our results further suggest that NPs produced in ACs may also modulate their own activity. PMID: 17566658
    39. GATA binding sites at -90 and -81 in the rat BNP promoter are essential for the in vivo response to Ang II. PMID: 17587490
    40. BNP is a specific HIF-1alpha target gene PMID: 17822384
    41. Systemic BNP enhance reflex activation of cardiac vagal motoneurons, in parallel with the enhanced reflex bradycardia. Enhancement is due to an action before the vagal efferent arm of the reflex pathway. PMID: 17906112
    42. Partial left ventricular unloading regressed myocardial hypertrophy, reversed contractile dysfunction and normalized the mRNA BNP expression of failing rat hearts. PMID: 18006556
    43. Inflammation contributes specific cytokines, leading to the disregulation of cardiac ANF and BNP production observed during myocardial inflammation, and that this process is angiotensin receptor 1 dependent. PMID: 18408131
    44. Chromogranin B is a novel regulator of cardiomyocyte InsP(3)/Ca(2+)-dependent signaling, nuclear factor kappaB activity, and BNP production. PMID: 18420944
    45. Calcium imaging showed that application of brain natriuretic peptide (BNP) caused an increase in intracellular calcium concentrations at the axon terminal, and the increase monotonically decayed when the acting site was moved away from the axon terminal. PMID: 18499087
    46. BNP was capable of inhibiting VSMCs proliferation by NPRA/cGMP pathway PMID: 19104909
    47. YY1 activates hBNP transcription through interaction with histone deacetylase 2 in cardiac myocytes. PMID: 19139378

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  • 亚细胞定位：
    [Brain natriuretic peptide 45]: Secreted.
  • 蛋白家族：
    Natriuretic peptide family
  • 组织特异性：
    Expressed in the atria and ventricles, but at much lower levels than NPPA. Expression levels in the ventricles are slightly higher than in the atria. Very low levels of expression detected in the brain, hypothalamaus, lung and aorta.; [Brain natriuretic p
  • 数据库链接：

    KEGG: rno:25105

    STRING: 10116.ENSRNOP00000010778

    UniGene: Rn.3835