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  4. Recombinant Rat Neutral ceramidase (Asah2), partial

Recombinant Rat Neutral ceramidase (Asah2), partial

  • 中文名称：
    Recombinant Rat Neutral ceramidase(Asah2),partial,Yeast
  • 货号：
    CSB-YP838699RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Neutral ceramidase(Asah2),partial,Yeast
  • 货号：
    CSB-EP838699RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Neutral ceramidase(Asah2),partial,Yeast
  • 货号：
    CSB-EP838699RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Neutral ceramidase(Asah2),partial,Yeast
  • 货号：
    CSB-BP838699RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Neutral ceramidase(Asah2),partial,Yeast
  • 货号：
    CSB-MP838699RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Asah2
  • Uniprot No.：
  • 别名：
    Asah2Neutral ceramidase; N-CDase; NCDase; EC 3.5.1.-; EC 3.5.1.23; Acylsphingosine deacylase 2; N-acylsphingosine amidohydrolase 2) [Cleaved into: Neutral ceramidase soluble form]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells. Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells. This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract.
  • 基因功能参考文献：
    1. Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA. PMID: 21613224
    2. mucin box and signal/anchor sequence of a rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membranes of mammalian cells PMID: 21597176
    3. Data show that conserved hydrophobic amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization, and enzyme activity of neutral ceramidase. PMID: 15123644
    4. Intestinal ceramidase thus is a neutral ceramidase 2 released by bile salts and resistant to pancreatic proteases and well suited to metabolize ceramide formed from sphingolipids to generate other bioactive sphingolipid messengers. PMID: 15217782
    5. the present study identified a novel amidase sequence containing a critical serine residue that may function as a nucleophile in the hydrolytic attack on the amide bond present in ceramide PMID: 16229686
    6. The presence of bile salts is important for maintaining high intraluminal levels of Alk-SMase and N-CDase, two key enzymes for hydrolysis of sphingomyelin in the gut. PMID: 17204455
    7. Chronic activation of neutral ceramidase protects beta-cells against cytokine-induced apoptosis. PMID: 18430368
    8. insights into the actions of Pseudomonas aeruginosa CDases and eukaryotic neutral CDases as an exotoxin and mediators of sphingolipid signaling are also revealed, respectively PMID: 19088069

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  • 亚细胞定位：
    [Neutral ceramidase]: Cell membrane; Single-pass type II membrane protein. Membrane raft; Single-pass type II membrane protein. Membrane, caveola; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Mitochondrion. Secreted, extracellular exosome.; [Neutral ceramidase soluble form]: Secreted.
  • 蛋白家族：
    Neutral ceramidase family
  • 组织特异性：
    Highly expressed in brain, kidney and heart. Expressed at lower level in other tissues such as liver. Expressed in intestine, kidney and liver (at protein level). Localizes in the epithelia of the jejunum and ileum.
  • 数据库链接：

    KEGG: rno:114104

    STRING: 10116.ENSRNOP00000016688

    UniGene: Rn.156958