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  4. Recombinant Rat Nucleolar RNA helicase 2 (Ddx21)

Recombinant Rat Nucleolar RNA helicase 2 (Ddx21)

  • 货号：
    CSB-YP662877RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP662877RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP662877RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP662877RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP662877RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ddx21; Ddx21aNucleolar RNA helicase 2; EC 3.6.4.13; DEAD box protein 21; Gu-alpha; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-782
  • 氨基酸序列
    MPGKLRSASK SESEGTEESM ETLQKPSEKK TRKEKPKSKT DEATEGVEEA ASSKVKAVKK KGPSEDDVGP PKSKKAKKQE EEPQDDPASK SKTSKKKKEP LEKKAPSAKT KEMKAEEPSE EEADAPKPKK TKKGKEANGD VGEKSPGLKN GLSHPKPDSS STQAPGEESE TEKEIPVEQK EGAFSNFPIS EETVKLLKAR GVNFLFPIQA KTFHHVYSGK DLIAQARTGT GKTFSFAIPL IEKLQGGLQE RKRGRAPQVL VLAPTRELAN QVSKDFSDIT KKLSVACFYG GTPYGGQIER MRSGIDILVG TPGRIKDHLQ NGKLDLTKLK HVVLDEVDQM LDMGFADQVE EILCVAYKKD SEDNPQTLLF SATCPHWVFN VAKKYMKSTY EQVDLIGKKT QKAAITVEHL AIKCHWTERA AVIGDVIRVY SGHQGRTIIF CETKKDAQEL SQNTCIKQDA QSLHGDIPQK QREITLKGFR NGNFGVLVAT NVAARGLDIP EVDLVVQSCP PKDVESYIHR SGRTGRAGRT GVCICFYQHK EEYQLAQVEQ KAGIKFKRIG VPSATEIIKA SSKDAIRLLD SVPPTAIGHF KQSAEKLIEE KGAVEALAAA LAHISGATSV DQRSLINSQA GFVTMILRCS VEMPNISYAW KELKEQLGES IDAKVKGMVF LKGKLGVCFD VRTEAVTEIK EKWHDSRRWQ LTVATEQPEL EGPPEGYRGG RGQRDGSRGS FRGQRGGSRN FRGQGQRGGS RNFRGQRPGG GNKSNRSPNK GQKRSFSKAF GQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes. Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'. Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Together with SIRT7, required to prevent R-loop-associated DNA damage and transcription-associated genomic instability: deacetylation by SIRT7 activates the helicase activity, thereby overcoming R-loop-mediated stalling of RNA polymerases. Involved in rRNA processing. May bind to specific miRNA hairpins. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.
  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytosol. Mitochondrion.
  • 蛋白家族：
    DEAD box helicase family, DDX21/DDX50 subfamily
  • 数据库链接：

    KEGG: rno:317399

    STRING: 10116.ENSRNOP00000063494

    UniGene: Rn.162310