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  4. Recombinant Rat Osteocalcin (Bglap)

Recombinant Rat Osteocalcin (Bglap)

In Stock
  • 货号：
    CSB-EP002682RA
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Bglap
  • Uniprot No.：
  • 别名：
    Bglap; Bglap2; Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    32.6kDa
  • 表达区域：
    50-99aa
  • 氨基酸序列
    YLNNGLGAPAPYPDPLEPHREVCELNPNCDELADHIGFQDAYKRIYGTTV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    重组大鼠骨钙素（Bglap），也称为骨γ-羧基谷氨酸蛋白，是一种在骨细胞中高度表达的蛋白质，可以通过重组技术来生产。下面是一般的步骤：
    设计和合成基因：基于大鼠骨钙素的氨基酸序列，设计并合成含有Bglap基因的DNA序列。这可以通过基因合成公司或PCR方法完成。
    克隆基因：将Bglap基因插入到表达载体中，这可以通过PCR扩增和限制性内切酶切割的方法完成。建议选择一些经过验证的表达载体，如pET系列载体，这些载体中包含了一些促进蛋白表达的元件，如启动子、增强子、终止子等。
    转染宿主细胞：在细胞中转染含有Bglap基因的表达载体。这些宿主细胞可以选择常见的哺乳动物细胞系，如CHO细胞、HEK293细胞等。
    培养和表达蛋白：根据表达载体中的启动子和调节元件，诱导Bglap蛋白在转染的宿主细胞中表达。在培养期间，需要添加抗生素等来保持表达载体的稳定。
    蛋白质的纯化：通过细胞破碎和离心等方法提取含有Bglap蛋白的细胞裂解液。然后使用亲和层析、离子交换层析、凝胶过滤层析等方法对蛋白进行纯化。
    蛋白质的鉴定和活性检测：使用SDS-PAGE、Western blot等方法对纯化后的Bglap蛋白进行鉴定和定量。此外，可以使用免疫印迹、酶联免疫吸附试验（ELISA）等方法来检测其生物学活性和功能。
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 基因功能参考文献：
    1. These data suggest that Undercarboxylated osteocalcin may have other effects on skeletal muscle in addition to its insulin sensitizing effect. PMID: 27291707
    2. Uncarboxylated osteocalcin potentiates insulin secretion, inhibits Kv channels and increases [Ca(2+)]i. PMID: 27746193
    3. This rat model of complete loss of osteocalcin provides a platform for further understanding the role of osteocalcin in disease. PMID: 27483347
    4. gamma-Carboxylase inhibition prevents osteocalcin carboxylation and protects against diabetic cardiomyopathy, possibly through direct action on upregulated G protein-coupled receptor family C group 6 member A and indirectly via adiponectin. PMID: 27488359
    5. Bone Gla Protein PMID: 25185647
    6. In primary adipocytes, both carboxylated and uncarboxylated osteocalcin increased basal and insulin-stimulated glucose transport. PMID: 24554534
    7. The physiological changes associated with estrous cycle stage were not sufficient to cause a detectable difference in carboxylated or undercarboxylated osteocalcin concentrations in this study. PMID: 23817840
    8. Osteocalcin (OC)-containing neurons are distributed throughout the cerebellar nuclei; OC may play a role in modulating the neuroprotective function of osteopontin. PMID: 22552891
    9. warfarin impaired the carboxylation of osteocalcin in rats. Factor Xa inhibitor edoxaban at or higher doses than needed for an antithrombotic effect sustained the circulating Gla-osteocalcin level. PMID: 22999414
    10. results suggest that under some physiological conditions the oncoprotein MDM2 may cooperate with p53 to regulate the osteocalcin gene during osteoblastic differentiation. PMID: 22405968
    11. These data are strongly in support of Sp1 as an essential transcription factor required for Osx recruitment and transactivation of the OCN promoter. PMID: 21820092
    12. purified Bone Gla Protein (BGP) resulted in stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha) in chondrocytes and vascular smooth muscle cells PMID: 21757657
    13. The mechanism of high glucose induced calcification in vascular smooth muscle cells may be due to the increased expression of cbfalpha-1 and OC. PMID: 21302441
    14. functional link between vitamin D-dependent bone tissue-specific transcription and histone acetylation at osteocalcin locus PMID: 11893738
    15. the VDRE in the distal region of the OC gene promoter is refractory to binding of the VDR-RXR complex when organized in a nucleosomal context PMID: 11964167
    16. deltaEF1 suppresses expression of the endogenous osteocalcin gene PMID: 12193549
    17. acetylation of histones H3 and H4 is functionally coupled to the chromatin remodeling events that mediate the developmental induction of osteocalcin gene transcription in bone cells PMID: 12554783
    18. Type I collagen promotes the activity of dentin formation by dental pulp cells by down-regulating Dmp-1 gene expression and up-regulating osteocalcin gene expression. PMID: 12647294
    19. A human vitamin D receptor-glucocorticoid receptor DNA-binding domain chimera is functional on rat osteocalcin vitamin D-responsive element with only conservative change of lys-49 to arg, and of negatively charged glu-53 to basic amino acid (lys or arg). PMID: 12960019
    20. Data show that in osteoblastic cells, the vitamin D receptor interacts directly with Runx2, and that this interaction contributes significantly to vitamin D3-dependent enhancement of the osteocalcin promoter. PMID: 15456860
    21. Results describe gene regulation by Dlx3 in relation to that of Msx2 and Dlx5 during osteoblast differentiation. PMID: 15456894
    22. Ocn is a Nurr1 target gene, which positions Nurr1 in the core of transcriptional factors regulating osteoblastic gene expression PMID: 15485875
    23. OC-IR nerve fibers in the circumvallate papilla mainly originated from the petrosal ganglion. PMID: 15621021
    24. Osteonectin (ON) and osteocalcin (OC) was used as osteoblast markersand assessed using an indirect immunoperoxidase. technique. PMID: 16443258
    25. CEBP-beta-dependent recruitment of SWI/SNF activity is required for chromatin remodeling and transcriptional activity of the bone-specific osteocalcin gene PMID: 16772287
    26. specific protein-DNA and protein-protein interactions that occur within the context of the OC gene promoter in osteoblastic cells stabilize the preferential association of the VDR-SRC-1 complex PMID: 17218095
    27. These results support a model where VDR preferentially recruits SRC-1 to enhance bone-specific OC gene transcription. PMID: 17786964
    28. These data suggest that long-term reduction in bone OC levels may induce the formation of immature bone, which is easily resorbed with changes in bone metabolism such as ovariectomy, and that OC may be one of the factors affecting bone turnover. PMID: 17968486
    29. TFIIA gamma together with ATF4 and Runx2 stimulates osteocalcin promoter activity and endogenous mRNA expression. PMID: 18171674
    30. Biomineralization followed secretion of osteocalcin, which may reflect early osteoblastic differentiation of cultured mesenchymal stem cells under osteoinductive conditions. PMID: 19191495
    31. The Notch-responsive Hes-1 protein is capable of repressing osteocalcin gene transcription in osteoblastic cells through an E-box in the proximal promoter. PMID: 19670267

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Osteocalcin/matrix Gla protein family
  • 数据库链接：

    KEGG: rno:25295

    STRING: 10116.ENSRNOP00000026530

    UniGene: Rn.9722