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  4. Recombinant Rat Prepronociceptin (Pnoc)

Recombinant Rat Prepronociceptin (Pnoc)

  • 货号：
    CSB-YP018278RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018278RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018278RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018278RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018278RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pnoc
  • Uniprot No.：
  • 别名：
    Pnoc; Prepronociceptin [Cleaved into: Nocistatin; Nociceptin; ORL1 receptor agonist; Orphanin FQ; PPNOC); Orphanin FQ2]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    98-132
  • 氨基酸序列
    MPR VRSVVQARDA EPEADAEPVA DEADEVEQKQ LQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.; Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.; Has potent analgesic activity.
  • 基因功能参考文献：
    1. LPS-inoculation in rodents is an unsuitable model for studying possible changes in the NOP-N/OFQ system in sepsis. PMID: 25161013
    2. Results suggest that CaMKII is involved in the inhibitory effect of N/OFQ on delayed rectifier potassium currents in acutely dissociated rat cerebral parietal cortical neurons PMID: 25171201
    3. Estradiol negatively modulates the pleiotropic inhibitory actions of nociceptin. PMID: 23735696
    4. These results suggest that N/OFQ can act directly in the nucleus accumbens shell to block cocaine-induced increases in extracellular dopamine levels PMID: 23219985
    5. The findings suggest a link between N/OFQ and the development of hyperalgesia and allodynia in a rat model of PTSD. PMID: 23082795
    6. The aim of the study was to determine by immunochemistry the expression of leptin, orexin A and orphanin FQ in the major salivary glands (parotid, submandibular and sublingual) of rat, sheep and cow. PMID: 23264212
    7. Orphanin FQ mRNA expression of hippocampus increased significantly after exposure to acute serious hypobaric hypoxia, but decreased after Morris water maze training. PMID: 21162294
    8. data provide new evidence for an interaction between the nociceptin and CRF systems in the central amygdala PMID: 22153590
    9. Data suggest that peripheral nociceptin/orphanin FQ/NOP receptor/ligand system contributes to abnormal pain sensitivity in inflammatory states. PMID: 22120202
    10. This study demonistrated that prepronociceptin, N/OFQ and nocistatin concentrations are significantly altered at different tissues of two rat neuropathy pain models. PMID: 22075222
    11. Inactivation of endogenous orphanin FQ nociceptin receptor oprl1 system attenuates the rewarding effect of opioids and therefore offers a therapeutic target for the treatment of drug abuse and addiction. PMID: 21095077
    12. In conclusion, our study indicates N/OFQ as a robust endogenous regulator of MCH neurons, which may play a role in anxiety and drug addiction. PMID: 21191090
    13. Inhibition of nociceptin in the substantia nigra of parkinsonian rats demonstrate its involvement (along with dopamine receptors) in the pathological and behavioral aspects of Parkinson disease. PMID: 21215744
    14. Conclude that the N/OFQ system promotes feeding by affecting the need to replenish lacking calories and by reducing aversive responsiveness. PMID: 20427724
    15. The results obtained, demonstrating the presence of N/OFQ in some alpha-MSH- and in many orexin-containing hypothalamic neurones, suggest a functional relationship between these neuropeptides and N/OFQ in the control of feeding behaviour and body weight. PMID: 20025627
    16. These data suggest that the ERK-1/2 pathway, at least in part, mediates the inhibitory effect of N/OFQ on the I(K) in acutely dissociated rat cerebral parietal cortical neurons. PMID: 20331962
    17. these findings suggest that corticotropin-releasing factor and the nociceptin/orphanin FQ/NOP system interact in the dorsal raphe nucleus during stress to control 5HT transmission; this may play a role in stress-related neuropsychopathologies PMID: 19747494
    18. These results suggest that the N/OFQ-NOP receptor system is affected by acute stress exposure, particularly in limbic regions. PMID: 19720395
    19. Hypothermia induced by orphanin fq/nociceptin is mediated by its own receptor PMID: 11406123
    20. Nociceptin/OFQ reduces the excitability of thalamic reticular nucleus and ventrobasal complex neurons predominantly through an increase of G-protein-coupled inwardly rectifying K(+) conductance PMID: 11826101
    21. Early life experiences such as maternal separation induce long-term effects on behavior as well as brain orphanin FQ peptide levels. PMID: 12076731
    22. orphanin FQ/nociceptin is involved in modulating pain transmission PMID: 12126889
    23. Levels of nociceptin mRNA and immunoreactivity in astrocytes cultured from neonatal forebrains are increased after exposure of the cells to inflammatory mediators LPS, TNF-alpha, and IL-1beta. PMID: 12203390
    24. Nociceptin gene induction is mediated by ERK and p38 MAP kinases. ERK is induced by protein kinase C and causes CREB phosphorylation. NFkappaB also appears to be crucial for ceramide-induced nociceptin expression. PMID: 12395108
    25. Orphanin FQ affects small fiber-mediated nociceptive responses and may behave as a complex modulator of pain systems in the brain stem. PMID: 12677331
    26. Expression of preproN/orphanin FQ is revealed in a distinct minor subpopulation of very small dorsal root ganglion neurons. PMID: 12932825
    27. The bed nucleus of the stria terminalis is involved in the modulation of CRF-induced anorexia, which is prevented by activation of N/OFQ receptors PMID: 14561874
    28. NOP receptors in the substantia nigra pars reticulata, activated by endogenous N/OFQ, drive a physiologically inhibitory control on motor behavior, possibly via modulation of the nigrostriatal dopaminergic pathway. PMID: 15282268
    29. Endogenous N/OFQ in substantia nigra sustains akinesia generated by impaired dopamine transmission and subthalamic nucleus overactivation. Nociceptin receptor antagonists may be beneficial in symptomatic therapy of parkinsonism. PMID: 15584926
    30. Thus, in addition to the classical opioid system, nociceptin, through opioid receptor-like-1 receptor stimulation in the rVLM, participates in the modulatory influence of EA on reflex-induced increases in blood pressure. PMID: 15649868
    31. Agouti related protein neurons in the arcuate nucleus also express the NOP1 receptor (Oprl) and nociceptin (Pnoc). PMID: 15890775
    32. N/OFQ contributes to neurally mediated gastric mucosal protection PMID: 15919744
    33. the stimulatory effect of N/OFQ on consumption may arise from this peptide's inhibitory influence on activity of anorexigenic pathways containing alpha-MSH PMID: 16584812
    34. OFQ/N plays a physiologically significant role in the regulation of prolactin secretion during lactation, and it mediates its effects via actions at the ORL-1 receptor subtype. PMID: 16887913
    35. Immunoreactivity was seen in the tunnel crossing fibers and the large boutons terminating onto outer hair cells, and in the fibers terminating onto the afferents to the inner hair cells. PMID: 16935273
    36. In rat progenitor and mouse neuroblastoma cell lines, agents that increase prepronociceptin mRNA expression also induce neurite outgrowth, suggesting a close relationship between prepronociceptin and cellular differentiation. PMID: 16935438
    37. endogenously released OFQ/N interacts with noradrenergic activity within the basolateral amygdala in modulating memory consolidation. PMID: 17202427
    38. Nociceptin has a central role in estradiol-only induced sexual receptivity. PMID: 17274997
    39. The present study suggested that N/OFQ inhibited I(K) and PKC was involved in N/OFQ-evoked response in acutely dissociated rat cerebral parietal cortical neurons. PMID: 17382298
    40. Results show that nociceptin/orphaninFQ levels are altered by treatment with cocaine. PMID: 17478889
    41. Changes in nociceptin and its receptor in spinal dorsal horn during electroacupuncture treatment for peripheral inflammatory pain were studied. PMID: 17493706
    42. kappa-opioids and orphanin FW (OFQ) both decreased 5-HT efflux in the CNS; in contrast to kappa-opioids, which reversed mu-opioid-induced increases in 5-HT efflux, anti-mu-opioid effects of OFQ apparently do not involve changes in 5-HT transmission PMID: 17499930
    43. Role of hypothalamus nociceptin/orphanin FQ in pre-ovulatory luteinizing hormone surge of estrogen and progesterone-primed, ovariectomized rats. PMID: 17640482
    44. Activation of ORL-1 receptors on myenteric neurons by nociceptin reduces excitatory and inhibitory neurotransmission within the gastrointestinal tract to alter peristalsis. PMID: 17681177
    45. Therefore, N/OFQ-NOP has an important role in microvascular inflammation, and this may be targeted during disease, particularly as we have proven that UFP-101 is an effective antagonist of microvascular responses in vivo. PMID: 17766480
    46. Our findings suggest that LPS regulates N/OFN expression in sensory neurons via a novel combination of LPS receptor components and demonstrate for the first time a direct action of a key initiator of innate immune responses on neurons. PMID: 18027846
    47. Results suggest nociceptin improves the impairment of learning and memory and blocks the mecamylamine-induced decrease in acetylcholine release, and that these effects may not be mediated by NOP receptors. PMID: 18191820
    48. The observations indicate that cardiac noxious neural afferent drive is responsible for the up-regulation of N/OFQ in the primary afferent neurons and intrinsic spinal neurons. PMID: 18280651
    49. Chronic high-NaCl intake prolongs the cardiorenal responses to central nociceptin and produces regional changes in the endogenous brain NOP receptor system. PMID: 18987291
    50. strains with low content of Cart gene expression had elevated N/OFQ-induced feeding, but contrary to expectations, strain 10, with the lowest Cart gene expression, exhibited low N/OFQ-induced feeding, on the order of that of the WOKW rats PMID: 19070636

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Opioid neuropeptide precursor family
  • 组织特异性：
    Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary.
  • 数据库链接：

    KEGG: rno:25516

    STRING: 10116.ENSRNOP00000019117

    UniGene: Rn.87935