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  4. Recombinant Rat Prostaglandin G/H synthase 2 (Ptgs2)

Recombinant Rat Prostaglandin G/H synthase 2 (Ptgs2)

  • 中文名称：
    大鼠Ptgs2重组蛋白
  • 货号：
    CSB-YP018986RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Ptgs2重组蛋白
  • 货号：
    CSB-EP018986RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Ptgs2重组蛋白
  • 货号：
    CSB-EP018986RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Ptgs2重组蛋白
  • 货号：
    CSB-BP018986RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Ptgs2重组蛋白
  • 货号：
    CSB-MP018986RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ptgs2; Cox-2; Cox2; Prostaglandin G/H synthase 2; EC 1.14.99.1; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    18-604
  • 氨基酸序列
    ANP CCSNPCQNRG ECMSIGFDQY KCDCTRTGFY GENCTTPEFL TRIKLLLKPT PNTVHYILTH FKGVWNIVNN IPFLRNSIMR YVLTSRSHLI DSPPTYNVHY GYKSWEAFSN LSYYTRALPP VADDCPTPMG VKGNKELPDS KEVLEKVLLR REFIPDPQGT NMMFAFFAQH FTHQFFKTDQ KRGPGFTRGL GHGVDLNHVY GETLDRQHKL RLFQDGKLKY QVIGGEVYPP TVKDTQVDMI YPPHVPEHLR FAVGQEVFGL VPGLMMYATI WLREHNRVCD ILKQEHPEWD DERLFQTSRL ILIGETIKIV IEDYVQHLSG YHFKLKFDPE LLFNQQFQYQ NRIASEFNTL YHWHPLLPDT FNIEDQEYTF KQFLYNNSIL LEHGLAHFVE SFTRQIAGRV AGGRNVPIAV QAVAKASIDQ SREMKYQSLN EYRKRFSLKP YTSFEELTGE KEMAAELKAL YHDIDAMELY PALLVEKPRP DAIFGETMVE LGAPFSLKGL MGNPICSPQY WKPSTFGGEV GFRIINTASI QSLICNNVKG CPFASFNVQD PQPTKTATIN ASASHSRLDD INPTVLIKRR STEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia.
  • 基因功能参考文献：
    1. Increased expression of COX-2 is associated with aortic endothelial dysfunction. PMID: 30292829
    2. findings indicated that BMP9 is involved in the phosphate-induced calcification in VSMCs and COX-2 partly mediates the BMP9-induced calcification in VSMCs through activating Wnt/beta-catenin pathway. PMID: 29073723
    3. the cortical COX2 pathway was activated in CUMS rats. Inhibition of COX2 activity/expression can obviously improve depressive behaviours in CUMS rats. Upregulating COX2 expression can increase the susceptibility of rats to CUMS. PMID: 28352129
    4. The present study proposed that COX-2-mediated inflammation is important as a candidate target for hippocampal impairment in the hypothyroidism. PMID: 29436670
    5. COX1 and 2 gene expression and localization in the injured brain regulating prostaglandin synthase. PMID: 28933223
    6. In heart irradiation groups, 3-fold up-regulation of both ET-1 and COX-2 was observed. In pelvis irradiation groups, 3-fold up-regulation of ET-1 was seen, but not significant changes in COX-2 gene expression have observed at distant heart tissues after pelvis irradiation. PMID: 28508833
    7. Microglial TNFalpha induces cyclooxygenase 2 (COX2) and PGI2 synthase expression in spinal endothelial cells during neuropathic pain. These findings further suggest that the glia-endothelial cell interaction of the neurovascular unit via transient TNFalpha is involved in the generation of neuropathic pain. PMID: 28451639
    8. The s measured brain oxygenation in localized areas from freely-moving rodents and discovered a severe hypoxic event (pO2 < 10 mmHg) after the termination of seizures. This event lasted over an hour, is mediated by hypoperfusion, generalizes to people with epilepsy, and is attenuated by inhibiting cyclooxygenase-2 or L-type calcium channels. PMID: 27874832
    9. C2-ceramide enhances phenylephrine-induced vascular smooth muscle constriction by mediation of the ER stress/COX-2/PGE2 pathway. PMID: 28797762
    10. Results point to an excess of reactive oxygen species mainly from NAD(P)H oxidase after aluminum exposure and increased vascular prostanoids from COX-2 acting in concert to decrease NO bioavailability, thus inducing vascular dysfunction and increasing blood pressure. PMID: 28826906
    11. Hepatic COX2 expression and PGI2 production are enhanced in cirrhotic rats, but the correlation with Hepatic encephalopathy (HE) is not remarkable. PMID: 27580512
    12. a stiffness-dependent YAP/TAZ-mediated positive feedback loop that drives remodeling phenotypes in pulmonary artery smooth muscle cells via reduced COX-2 and prostaglandin activity. PMID: 28642262
    13. the disrupted pattern of hippocampal neurogenesis induced by MNU is different between developmental and postpubertal exposure and is dependant on COX2 regulation. PMID: 28688903
    14. the long-lasting oligemia following cortical spreading depression consists of at least two distinct temporal phases, mediated by preferential actions of COX-1- and COX-2-derived prostanoids: an initial phase mediated by COX-1 that involves TXA2 followed by a later phase, mediated by COX-2 and PGF2alpha. PMID: 27178425
    15. These data suggest that adaptive changes induced in the myocardium by CIH may include activation of cPLA2alpha and COX-2 via beta2-AR/Gi-mediated stimulation of the ERK/p38 pathway. PMID: 27686454
    16. COX-2 protein expression was upregulated in lung tissue in response to skeletal muscle ischemia reperfusion PMID: 26724476
    17. It was concluded that intestinal damage during acute pancreatitis is exacerbated in elderly rats compared with young rats and that COX-2 inhibition could be a potential therapeutic target to offer tailored treatment for acute pancreatitis in the elderly. PMID: 26610611
    18. TNFalpha-RelB/p52 pathway may be involved in the early stages of renal damage, in part by stimulating COX-2 and inflammatory responses PMID: 26824492
    19. COX1/2 inhibition alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury PMID: 26612417
    20. Low-salt diet modulates mesenteric vascular responses via increased NO bioavailability suggested by increased iNOS protein expression and vasoconstrictor prostanoid production via COX-2. PMID: 26685759
    21. These results suggest the existence of a causal link between Cox-2 and p53, which may represent a toxic mechanism of electrophilic lipid peroxidation products. PMID: 25506925
    22. Src modulates the 4-Hydroxynonenal-enhanced activation of AP-1 and the expression of COX-2 PMID: 26466383
    23. IL-1beta reduces tonic contraction of mesenteric lymphatic muscle cells, with the involvement of COX-2 and prostaglandin E2 PMID: 25989136
    24. Data show that mesenchymal stem cells (MSCs) administration alleviated airway inflammation and emphysema through the down-regulation of cyclooxygenase-2 (COX-2) via p38 and ERK MAPK pathways. PMID: 25736434
    25. increased COX-2-dependent vasoconstriction contributes to renal endothelial dysfunction through enhanced reactive oxygen species generation in obesity. PMID: 25841778
    26. The aim of this study was to determine the role of COX-2 in the regulation of blood pressure and to define the mechanisms in two kidney one-clip hypertensive (2K1C) rats PMID: 25846103
    27. Treatment with probiotics has the potential of providing protection against colon cancer by down-regulating the COX-2 expression as one of the protective mechanisms. PMID: 25811420
    28. TNF-alpha-induced COX-2 and PGE2 stimulate Wnt signaling and activate Wnt target genes PMID: 26123748
    29. Increased COX2 expression after l-DOPA long-term treatment in Parkinsonian-like rats could contribute to the development of dyskinesia. PMID: 26009769
    30. Concomitant use of alpha-lipoic acid and cyclosporine significantly increased nitric oxide production, cyclooxygenase-2 and miR-210 gene expression in rat colon. PMID: 26276312
    31. E2-BSA induced the COX-2 expression and consequent PGE2 and PGF2alpha release in rat OECs. These effects are mediated through GPR30-derived EGFR transactivation and PI-3K/Akt cascade leading to NF-kappaB activation PMID: 26241029
    32. Low-level laser therapy decreases Achilles tendon inflammatory process mediated by Il1b and COX-2. PMID: 25070591
    33. COX-2 oxidative metabolism is a key regulator of the anandamide-mediated decidual remodeling in pregnancy. PMID: 26335727
    34. Our results demonstrated that ventricular hypertrophy abrogated isoflurane-induced delayed cardioprotection by alteration of iNOS/COX-2 pathway PMID: 25400168
    35. Hypomethylation of PTGS2, and hypermethylation of APC2 may be causally linked to the etiology of oral cancer in a rat model. PMID: 25635769
    36. Clathrin-dependent internalization of the angiotensin II ATA receptor links receptor internalization to COX-2 protein expression in rat aortic vascular smooth muscle cells PMID: 25542758
    37. Report design of benzimidazole analogs endowed with oxadiazole as selective COX-2 inhibitors. PMID: 25303727
    38. Cadmum induced release of reactive oxygen species derived from NADPH oxidase may be due to the increased local release of angiotensin II and the release of vasoconstrictor prostanoids derived from the COX-2 pathway. PMID: 23973752
    39. Data suggest that cyclooxygenase-2 (COX2) and NADPHox are potential therapeutic targets to decrease testosterone-driven cardiovascular risk. PMID: 25700020
    40. data propose that an increase in COX-2 expression in ventral CA1 following trauma is likely due to its attenuated degradation. PMID: 25058273
    41. Data (including data from studies in transgenic rats) suggest that attenuation of circadian clock system, especially its core component Rev-erb-alpha, contributes to induction of Ptgs2 in endometrial stromal cells during decidualization/placentation. PMID: 25648833
    42. Nitric oxide synthase (NOS) and cyclooxygenase (COX) isoform expression patterns were determined in femoral artery of obese Zucker rats. PMID: 25216050
    43. Reflux of duodenal contents induces COX2 expression and increases prostaglandin synthesis in dysplastic and cancer tissues PMID: 24914375
    44. ischemia reperfusion injury significantly increased the COX2 expression, PGI2 and TXA2 levels, and the PGI2/TXA2 ratio in rat hippocampus in a time-dependent manner PMID: 25388440
    45. The present study showed that the group receiving Vitamin E supplementation at 3x recommended daily intake displayed reduced COX2 expression. PMID: 25123248
    46. during the early phase of ANG II-dependent hypertension, the increased (P)RR and COX-2 expression in the renal medulla may contribute to attenuate the vasoconstrictor effects of ANG II on renal hemodynamics PMID: 25143455
    47. The data suggest that local hypertonicity in the medullary thick ascending limb is associated with an increase in COX-2 expression concomitant with elevated EP3 receptor expression, which limits COX-2 activity in this segment of the nephron. PMID: 25080527
    48. Elevated COX-2 expression during hypoxia where angiogenesis occurs and re-oxygenation, when micro-vessels regress, identifies this protein as a vascular remodeling molecule crucial for angioplasticity. PMID: 24796880
    49. Fibroblasts activated by hepatoma cell supernatant express COX-2 and HGF at higher levels. PMID: 24815169
    50. Suggest lead-associated inflammatory neurotoxicity occurs via activation of pro-inflammatory NFAT3/COX-2 axis. PMID: 25193092

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  • 亚细胞定位：
    Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus inner membrane; Peripheral membrane protein. Nucleus outer membrane; Peripheral membrane protein.
  • 蛋白家族：
    Prostaglandin G/H synthase family
  • 组织特异性：
    Expressed throughout the forebrain in discrete populations of neurons and is enriched in the cortex and hippocampus.
  • 数据库链接：

    KEGG: rno:29527

    STRING: 10116.ENSRNOP00000003567

    UniGene: Rn.217585