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  4. Recombinant Rat Protein-lysine 6-oxidase (Lox)

Recombinant Rat Protein-lysine 6-oxidase (Lox)

  • 货号：
    CSB-YP013038RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013038RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013038RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013038RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    LoxProtein-lysine 6-oxidase; EC 1.4.3.13; Lysyl oxidase) [Cleaved into: Protein-lysine 6-oxidase; long form; Protein-lysine 6-oxidase; short form]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    163-411
  • 氨基酸序列
    DDPYNPYK YSDDNPYYNY YDTYERPRSG SRHRPGYGTG YFQYGLPDLV PDPYYIQAST YVQKMSMYNL RCAAEENCLA SSAYRADVRD YDHRVLLRFP QRVKNQGTSD FLPSRPRYSW EWHSCHQHYH SMDEFSHYDL LDASTQRRVA EGHKASFCLE DTSCDYGYHR RFACTAHTQG LSPGCYDTYA ADIDCQWIDI TDVQPGNYIL KVSVNPSYLV PESDYSNNVV RCEIRYTGHH AYASGCTISP Y
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture.
  • 基因功能参考文献：
    1. data suggest a detrimental role for the chronic increase of cardiac LOX expression and activity in the transition from compensated remodeling to decompensated failure. PMID: 28668305
    2. Together, these findings suggest that bone fragility in DM2 is initiated in the matrix phase and that the LOX family may play a critical part in the pathogenesis of DM2 mediated bone fragility. PMID: 29146187
    3. Here we show that orthotopic implantation of rat prostate AT-1 tumour cells increased LOX and LOXLs mRNA expressions in the tumour and in the surrounding non-malignant prostate tissue PMID: 26804196
    4. The reduction of leptin levels by BAPN in vivo and the ability of this compound to inhibit leptin-induced profibrotic mediators and ROS production in cardiac and vascular cells suggest that interactions between leptin and LOX regulate downstream events responsible for myocardial and vascular fibrosis in obesity. PMID: 26780438
    5. LOX can promote the progress of epithelial-to-mesenchymal transition in a rat model of pulmonary fibrosis. PMID: 26670953
    6. In transgenic mice expressing rat LOX in wild-type megakaryocytes and platelets, higher affinity of Pf4-Lox(tg/tg) platelets to the collagen sequence GFOGER implies that the collagen receptor integrin alpha2beta1 is affected by LOX. PMID: 26755713
    7. Silenced lysyl oxidase expression results the growth of C3H10T1/2 cells by 50%, and blocked osteoblast differentiation. PMID: 24971753
    8. Decreased lox expression in diaphragmatic development and lung branching morphogenesis may contribute to the diaphragmatic defect and impaired airway formation in the nitrofen-induced congenital diaphragmatic hernia model. PMID: 25111273
    9. Results indicate that polyphenols bind to monomeric tropoelastin and enhance coacervation, aid in crosslinking of elastin by increasing lysyl oxidase (LOX) synthesis, and by blocking MMP-2 activity. PMID: 24440697
    10. Study supports a novel mechanism of n-acetylcysteine alleviating idiopathic pulmonary fibrosis by inhibition of LOX activity in a rat model. Time course of LOX activity during bleomycin-induced pulmonary fibrosis was also examined. PMID: 23006535
    11. These results suggest that in an indirect co-culture system, pelvic ligament fibroblasts with mechanical stretch stimulation can promote bone mesenchymal stem cell differentiation, reflecting in the increased expression of elastin, LOX, and Fibulin-5. PMID: 22205540
    12. These findings indicate that TNF-alpha stimulated LOX expression may play an important role in progressive cardiac fibrosis. PMID: 21893029
    13. In cardiac fibroblasts, Rac1 GTPase mediates upregulation of fibronectin via LOX and CTGF. PMID: 21215756
    14. The data presented herein regarding chromatin remodeling indirectly support the hypothesis that LOX binds to histone H1 in vivo. PMID: 20888776
    15. LOX were overexpressed in late stages of intimal hyperplasia in the allografts PMID: 20606470
    16. Suggest that uric acid increases fibronectin synthesis both in vivo and in vitro via urate transporters through upregulation of lysyl oxidase expression. PMID: 20484295
    17. lysyl oxidase propeptide contains both N- and O-linked carbohydrates; its structure is a mostly disordered protein PMID: 20192271
    18. Data indicate that lysyl oxidase propeptide (LOX-PP) inhibits terminal mineralization in primary calvaria osteoblast cultures when added at early stages of differentiation, with no effects seen when present at later stages. PMID: 20048148
    19. a PKC-MEK-MAPK-dependent pathway is critical to the enhanced expression of the LO gene PMID: 11968017
    20. findings indicate control of lysyl oxidase at endocrine, paracrine, and autocrine levels within the ovary and suggest coordinated regulation of ovarian extracellular matrix during follicular development PMID: 12488341
    21. comparison and aa sequence alignment of human and rat lysyl oxidase and lysyl oxidase-like gene (LOXL1); role in formation and repair of ECM elastin and collagen PMID: 12577300
    22. Beta-catenin transformation pathway is activated by lysyl oxidase down-regulation. PMID: 12686140
    23. The results showed that LOX was expressed in the choroid plexus, blood vessel walls, brain matrix, and neurons of normal rat. PMID: 14741400
    24. Upregulation by cigarette smoke condensate(CSC) of cellular thiols may play an important role in the downregulation of lysyl oxidase and subsequently destabilization of the lung ECM in CS-induced emphysema. PMID: 15509664
    25. Downregulation of LO is linked with upregulation of other Cu-binding proteins and with alteration in Cu homeostasis in the cadmium resistant lung fibroblasts. PMID: 17584760
    26. These novel data suggest that LOX-PP may provide a feedback control mechanism that serves to inhibit properties associated with the development of vascular pathology. PMID: 18060869
    27. spatial and temporal expression of LOX and LOXL1 during growth and aging in the aorta and specific roles for LOX and LOXL1 in the synthesis and remodeling of elastic and collagen fibers PMID: 18803461

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  • 亚细胞定位：
    Secreted. Secreted, extracellular space.
  • 蛋白家族：
    Lysyl oxidase family
  • 组织特异性：
    Aorta and lung.
  • 数据库链接：

    KEGG: rno:24914

    STRING: 10116.ENSRNOP00000065314

    UniGene: Rn.11372