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  4. Recombinant Rat Protein phosphatase 1 regulatory subunit 15A (Ppp1r15a)

Recombinant Rat Protein phosphatase 1 regulatory subunit 15A (Ppp1r15a)

  • 货号：
    CSB-YP746925RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP746925RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP746925RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP746925RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP746925RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ppp1r15a; Gadd34; Myd116; Peg3; Protein phosphatase 1 regulatory subunit 15A; Growth arrest and DNA damage-inducible protein GADD34; Myeloid differentiation primary response protein MyD116 homolog; Progression elevated gene 3 protein; PEG-3
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-578
  • 氨基酸序列
    MAPSPRPQHV LHWKEAHSFY LLSPLMGFLS RAWSRLRGPE VSEAWLAETV AGANQIEADA LLTPPPVSEN HLPLRETEGN GTPEWSKAAQ RLCLDVEAQS SPPKTWGLSD IDEHNGKPGQ DGLREQEVEH TAGLPTLQPL HLQGADKKVG EVVAREEGVS ELAYPTSHWE GGPAEDEEDT ETVKKAHQAS AASIAPGYKP STSVYCPGEA EHRATEEKGT DNKAEPSGSH SRVWEYHTRE RPKQEGETKP EQHRAGQSHP CQNAEAEEGG PETSVCSGSA FLKAWVYRPG EDTEEEEDSD LDSAEEDTAH TCTTPHTSAF LKAWVYRPGE DTEEEDDGDW DSAEEDASQS CTTPHTSAFL KAWVYRPGED TEEEDDSENV APVDSETVDS CQSTQHCLPV EKTKGCGEAE PPPFQVAFYL PGQKPAPPWA APKLPLRLQK RLRSFKAPAR NQDPEIPLKG RKVHFSEKVT VHFLAVWAGP AQAARRGPWE QFARDRSRFA RRIAQAEEQL GPYLTPAFRA RAWTRLRNLP LPLSSSSLPL PEPCSSTEAT PLSQDVTTPS PLPSEIPPPS LDLGGRRG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.
  • 基因功能参考文献：
    1. Following restraint stress, there was rapid induction of GADD34 in brain. PMID: 23644055
    2. results show that GADD34 plays an important role in cell protection in mutant huntingtin expressing cells PMID: 21925170
    3. GADD34 has the potential to influence cell survival in ischaemically compromised tissue. PMID: 15713259
    4. After transient cerebral ischemia GADD34 protein levels were increased in the cortex but not in the hippocampal CA1 subfield; these changes occurred earlier in SOD1 transgenic than in wild-type animals; rise in gadd34 mRNA levels did not differ [GADD34] PMID: 15255948
    5. The inherent property of PEG-3 to function as a dominant negative of the growth inhibitory property of GADD34 might rescue cells from DNA damage-induced apoptosis leading to growth independence and tumorigenesis. PMID: 15674324
    6. PEG-3 does not exist in normal rat cells, which have a single diploid complement of Gadd34. Sequence analysis of the rat Gadd34 gene & comparison with PEG-3 indicates that PEG-3 is most likely a mutant of Gadd34. PMID: 12813455
    7. PEG-3 plays a central role in augmenting tumor progression by modulating several critical parameters of the carcinogenic process, such as genomic stability and local tumor cell invasion. PMID: 15389539

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    PPP1R15 family
  • 数据库链接：

    KEGG: rno:171071

    STRING: 10116.ENSRNOP00000048451

    UniGene: Rn.2232