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  4. Recombinant Rat Regulator of G-protein signaling 14 (Rgs14)

Recombinant Rat Regulator of G-protein signaling 14 (Rgs14)

  • 货号：
    CSB-YP019646RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019646RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019646RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019646RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019646RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rgs14; Regulator of G-protein signaling 14; RGS14
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-544
  • 氨基酸序列
    MPGKPKHLGV PNGRMVLAVS DGELTSTSGS QAQGEGRGSS LSIHSLPSGP SSPFSTDEQP VASWAQSFER LLQDPRGLAY FTEFLKKEFS AENVTFWQAC ERFQQIPASD TKQLAQEAHN IYHEFLSSQA LSPVNIDRQA WLSEEVLAQP RPDMFRAQQL QIFNLMKFDS YARFVKSPLY QECLLAEAEG RPLREPGSSH LGSPDTARKK PKLKPGKSLP LGVEELGQLP LAEGRPLRKS FRREMPGGAV NSALRRESQG SLNSSASLDL GFLAFVSSKS ESHRKSLGSG EGESESRPGK YCCVYLPDGT ASLALARPGL TIRDMLAGIC EKRGLSLPDI KVYLVGKEQK ALVLDQDCTV LADQEVRLEN RITFQLELVG LERVVRISAK PTKRLQEALQ PILAKHGLSL DQVVLHRPGE KQLVDLENLV SSVASQTLVL DTLPDAKTRE ASSIPPCRSQ GCLPRTQTKD SHLPPLSSSL SVEDASGSTG KRQTCDIEGL VELLNRVQSS GAHDQRGLLR KEDLVLPEFL QLPSQRPGSQ EAPP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o) alpha subunit GNAO1. Has GAP activity on GNAI0, GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division; required for completion of the first mitotic division of the embryo. Involved in visual memory processing capacity; when overexpressed in the V2 secondary visual cortex area. Involved in hippocampal-based learning and memory; acts as a suppressor of synaptic plasticity in CA2 neurons. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance.
  • 基因功能参考文献：
    1. Results show that endogenous RGS14 localizes to subcellular compartments not previously recognized in studies of recombinant RGS14 in unnatural host cells. The two most surprising observations were that RGS14 is not enriched at the plasma membrane, and that it is found within chromatin-rich regions of the nucleus in close proximity with active RNA Pol-II. PMID: 28934222
    2. RGS14 can form complexes with GPCRs in cells that are dependent on Galpha(i/o) and these RGS14.Galpha(i1).GPCR complexes may be substrates for other signaling partners such as Ric-8A PMID: 21880739
    3. RGS proteins with with GTPase-activating protein (GAP) activity affect both stress resistance and longevity in several species. PMID: 21255223
    4. Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits PMID: 11976690
    5. Substituting protein kinase A phosphorylation site Thr494 with either aspartate or glutamate to mimic phosphorylation enhances RGS14 inhibition of nucleotide exchange activity on G alpha(i) subunits almost 3-fold. PMID: 12534294
    6. RGS14 facilitates the formation of a selective Ras.GTP-Raf-MEK-ERK multiprotein complex to promote sustained ERK activation and regulate H-Ras-dependent neuritogenesis. PMID: 19319189

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  • 亚细胞定位：
    Nucleus. Nucleus, PML body. Cytoplasm. Membrane. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cell projection, dendrite. Cell projection, dendritic spine. Cell junction, synapse, postsynaptic density.
  • 组织特异性：
    Expressed in neurons of the V2 secondary visual cortex area (at protein level). Expressed at high levels in the brain cortex, hippocampus, striatum, thalamus and substantia nigra, in the lung, and spleen. Low expression has been found in heart, liver, ske
  • 数据库链接：

    KEGG: rno:114705

    STRING: 10116.ENSRNOP00000021596

    UniGene: Rn.9795