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  4. Recombinant Rat SNARE-associated protein Snapin (Snapin)

Recombinant Rat SNARE-associated protein Snapin (Snapin)

  • 货号：
    CSB-YP021882RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021882RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021882RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021882RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021882RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Snapin
  • Uniprot No.：
  • 别名：
    Snapin; Bloc1s7; Snap25bp; SnapapSNARE-associated protein Snapin; Biogenesis of lysosome-related organelles complex 1 subunit 7; BLOC-1 subunit 7; Synaptosomal-associated protein 25-binding protein; SNAP-associated protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-136
  • 氨基酸序列
    AAAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES QVELREQIDN LATELCRINE DQKVALDLDP YVKKLLNARR RVVLVNNILQ NAQERLRRLN HSVAKETARR RAMLDSGVYP PGSPSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells. As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor.
  • 基因功能参考文献：
    1. In pancreatic beta-cells the SNAP-25 accessory protein, snapin, C-terminal H2 domain binds SNAP-25 through its N-terminal Sn-1 domain. PMID: 26946359
    2. AC6-mediated inhibition of neurite outgrowth was reversed by the overexpression of Snap25 or a Snapin mutant that could not be phosphorylated. PMID: 21986494
    3. snapin may not have a role as a SNARE regulator in neurotransmitter release, as had been previously thought [SNAPIN] PMID: 15084593
    4. EBAG9 and Snapin have roles in controlling exocytosis processes PMID: 15635093
    5. our data suggest that snapin regulates dendrite number in developing neurons by modulating cypin-promoted microtubule assembly. PMID: 16120643
    6. Snapin binds to a 170-residue predicted ryanodine receptor cytosolic loop (RyR2 residues 4596-4765), containing a hydrophobic segment required for snapin interaction. PMID: 16723744
    7. activates alpha 1 Adrenergic Receptor and induces calcium influx through TRPC6 channels. PMID: 18480566

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  • 亚细胞定位：
    Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Cytoplasm, perinuclear region. Golgi apparatus membrane. Lysosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
  • 蛋白家族：
    SNAPIN family
  • 组织特异性：
    Strongly expressed in testis, spleen, kidney, liver and brain; lower expression in heart, fat and skeletal muscle.
  • 数据库链接：

    KEGG: rno:295217

    STRING: 10116.ENSRNOP00000018830

    UniGene: Rn.230579