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  4. Recombinant Rat Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Ppp3ca)

Recombinant Rat Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Ppp3ca)

  • 货号：
    CSB-YP018572RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018572RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018572RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018572RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018572RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ppp3ca; Calna; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; EC 3.1.3.16; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-521
  • 氨基酸序列
    SEPKAIDPK LSTTDRVVKA VPFPPSHRLT AKEVFDNDGK PRVDILKAHL MKEGRLEESV ALRIITEGAS ILRQEKNLLD IDAPVTVCGD IHGQFFDLMK LFEVGGSPAN TRYLFLGDYV DRGYFSIECV LYLWALKILY PKTLFLLRGN HECRHLTEYF TFKQECKIKY SERVYDACMD AFDCLPLAAL MNQQFLCVHG GLSPEINTLD DIRKLDRFKE PPAYGPMCDI LWSDPLEDFG NEKTQEHFTH NTVRGCSYFY SYPAVCDFLQ HNNLLSILRA HEAQDAGYRM YRKSQTTGFP SLITIFSAPN YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF TWSLPFVGEK VTEMLVNVLN ICSDDELGSE EDGFDGATAA ARKEVIRNKI RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLSGG KQTLQSATVE AIEADEAIKG FSPQHKITSF EEAKGLDRIN ERMPPRRDAM PSDANLNSIN KALASETNGT DSNGSNSSNI Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Dephosphorylates heat shock protein HSPB1. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32. May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins.
  • 基因功能参考文献：
    1. FKBP12 can endogenously regulate calcineurin activity with therapeutic implications for the treatment of Parkinson's Disease. PMID: 29229832
    2. 11R-VIVIT regulates the expression of NFATc1, and some other molecules in Calcineurin/NFAT signaling pathway PMID: 28478803
    3. Results show that acute hyperammonemia induces hypophosphorylation of neuronal and glial intermediate filaments proteins in cerebral cortex of 21-day-old rats without affecting the hippocampus. In slices of cerebral cortex of 21-day-old animals, the cytoskeleton of astrocytes is more susceptible than neurons to ammonia toxicity, and this effect is mediated by PP2B and PP1 activities. PMID: 26936604
    4. Calcineurin inhibitor tacrolimus ameliorated proteinuria in nephrotic model by inhibiting the redistribution of CN-A and nephrin at the slit diaphragm. PMID: 27009276
    5. modulation of Kv by PKA can be reversed by protein phosphatase 2B/calcineurin. PMID: 25793374
    6. Prolonged oxidative stress-induced activation of calcineurin contributes to the attenuation of AMPK signalling and inhibits autophagy in cardiomyocytes. PMID: 24434520
    7. Aerobic training did not cause any changes (p < 0.05) in CaN A or CaN B expression levels in either the soleus or the plantaris muscle. PMID: 23549689
    8. the gamma-8 P/A-rich region is essential for binding calcineurin, suggesting that the gamma-8/calcineurin complex may regulate AMPA receptor phosphorylation and trafficking PMID: 24418105
    9. PKCepsilon may negatively regulate adverse myocardial remodeling by cooperating with calcineurin to downregulate fibrosis and induce transcription of cardioprotective wound healing genes, including COX-2 PMID: 24298017
    10. Calcineurin is fine-tuned to unique bandwidths and compute input variables in an asymmetric manner. PMID: 23602566
    11. CNAalpha and CDK5 enzymatic activities are key determinants of release probability. PMID: 23699505
    12. Fluoride might up-regulate the mRNA and protein expression of CaN, and the changes in CaN level may be involved in the increase of the bone turnover and could be one of the pathogenetic factors in fluorosis. PMID: 23302338
    13. Homocysteine inhibits expression of 14-3-3epsilon and activates calcineurin in rat hippocampal neurons. PMID: 23139767
    14. Regulation of MEF2 transcriptional activity by calcineurin/mAKAP complexes. PMID: 23261540
    15. The expression levels of both the catalytic A and regulatory B subunits of calcineurin were increased at 1 and 2 weeks, but not at 9 weeks, after the induction of streptozotocin diabetes. PMID: 22305959
    16. hese data suggested an involvement of calcineurin in chronic constriction injury -elicited neuropathic pain. PMID: 21596102
    17. This result indicates that the interaction of PMCA4 with calcineurin A plays a regulatory role in the signalling during catecholamine secretion. PMID: 21740891
    18. we have identified calcineurin as a novel interaction partner for RAF1 and established a mechanistic link and possible therapeutic target for pathological cardiomyocyte hypertrophy induced by mutant RAF1 PMID: 21440552
    19. KChIP2 can attenuate cardiac hypertrophy possibly through modulation of intracellular calcium concentration and calcineurin/NFAT pathway PMID: 20051248
    20. Results describe the correlation between calcineurin and RANTES in rat apoptotic vascular smooth muscle cells stimulated by IFNgamma and CD40L. PMID: 20132097
    21. Data demonstrate that calcineurin activity is a major determinant of PGC-1alpha expression in skeletal muscle during diabetes and possibly other conditions associated with loss of muscle mass. PMID: 20359506
    22. Understanding alterations in CnA isoform distribution may help improve the targeting of current therapeutic interventions and/or the development of new treatments for TBI. PMID: 19751097
    23. These results reveal the importance of regulated calcineurin binding to mAKAPbeta for the induction of cardiac myocyte hypertrophy. PMID: 19883655
    24. cell-specific increase expression of myristoyltransferase and calcineurin in lung epithelium and endothelium PMID: 19916857
    25. There is cross-talk between the calcineurin and JNK pathway in controlling renovascular hypertension-induced cardiac hypertrophy. PMID: 18957384
    26. Calcineurin A alpha is significantly decreased in the particulate fractions but is not changed in the soluble fractions of the striatum of methamphetamine-sensitized rats. PMID: 11954050
    27. The functional differences conferred upon CaN by the alpha or beta catalytic subunit isoforms suggest that the alpha:beta and CaN:substrate ratios may determine the levels of CaN phosphatase activity toward specific substrates. PMID: 12135494
    28. CnA-alpha is upregulated in diabetic kidney glomeruli and collecting ducts and participates in regulation of AQP2 expression, phosphorylation, and function PMID: 15153553
    29. Increased intraocular pressure leads to the initiation of a calcineurin-mediated mitochondrial apoptotic retinal ganglion cell death pathway in glaucoma. PMID: 16103353
    30. The age-related formation of aggregates of the catalytic subunit of Cn was suggestive of a correlation between aggregate formation and diminished enzyme activity. The loss of Cn activity may alter signal transduction at synapses during the aging process. PMID: 16150427
    31. The isolation and transport of calcineurin Aalpha and calcineurin Abeta in rat aortic smooth muscle cells are reported. PMID: 17303652
    32. These findings further provide a link between the lipopolysaccharide-induced inflammatory response and the calcineurin/NFAT-3 signaling pathway that mediates the development of cardiac hypertrophy. PMID: 18398669
    33. These results suggest that ET-1-induced cardiac hypertrophy is inhibited by activation of PPAR-gamma, which is at least partly due to cross-talk between PPAR-gamma and calcineurin/NFAT. PMID: 18600431
    34. Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin. PMID: 19717566

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Cell membrane, sarcolemma. Cytoplasm, myofibril, sarcomere, Z line. Cell projection, dendritic spine.
  • 蛋白家族：
    PPP phosphatase family, PP-2B subfamily
  • 组织特异性：
    Expressed in neonatal cardiomyocytes (at protein level).
  • 数据库链接：

    KEGG: rno:24674

    STRING: 10116.ENSRNOP00000049674

    UniGene: Rn.228648