Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Sodium-dependent dopamine transporter (Slc6a3), partial

Recombinant Rat Sodium-dependent dopamine transporter (Slc6a3), partial

  • 中文名称：
    大鼠Slc6a3重组蛋白
  • 货号：
    CSB-YP021701RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Slc6a3重组蛋白
  • 货号：
    CSB-EP021701RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Slc6a3重组蛋白
  • 货号：
    CSB-BP021701RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Slc6a3重组蛋白
  • 货号：
    CSB-MP021701RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Slc6a3Sodium-dependent dopamine transporter; DA transporter; DAT; Solute carrier family 6 member 3
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (Probable). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling.
  • 基因功能参考文献：
    1. Results show that Gbetagamma activation regulates DAT activity by increasing dopamine (DA) efflux, and suggests that Gbetagamma promotes an efflux-willing state of the transporter and that the activation of a GPCR can lead to a Gbetagamma-dependent DA efflux. Furthermore, the cellular responses that occur following activation of a Galphaq-coupled receptor are the result of multiple signaling pathways mediated by Galpha... PMID: 28894302
    2. a new genetic rat model with a loss-of-function point mutation in the dopamine transporter (DAT) gene (Slc6a3_N157K) was used to systematically study the research domain criteria matrix. PMID: 28167616
    3. Study compared showing a clear-cut profile of either gambling proneness or aversion, found a selective down-regulation of i) serotonin transporter in prefrontal cortex, ii) tyrosine hydroxylase in ventral striatum, iii) dopamine transporter in lymphocytes was evidenced in "gambler" vs "non-gambler" rats. PMID: 27789384
    4. Together these results support the hypothesis that sigma1R agonists promote dissociation of sigma1R multimers into monomers, which then interact with DAT to stabilize an outward-facing DAT conformation and enhance cocaine binding. PMID: 28495886
    5. The present study suggested that amphetamine-induced circling behavior indicated striatal dopaminergic alterations and that dopamine transporter (DAT) and dopamine D2 receptor binding could be key markers for predicting motor dysfunction after mild focal ischemia. PMID: 26911894
    6. Increased expression of DAT is associated with visceral obesity PMID: 26707058
    7. Data show that Fischer 344 rats displayed greater tyrosine hydroxylase and dopamine transporter levels in the frontal cortex compared to the Lewis rats. PMID: 26658810
    8. Study indicated that enhanced infralimbic DAT function is a consequence of adolescent social stress exposure PMID: 26056032
    9. Ctr9, formerly identified as a nuclei protein, interacts with the dopamine transporter in the cytoplasmic compartment. PMID: 26048990
    10. Data suggest that expression of Dat1 and Vmat2 (vesicular monoamine transporter 2) in dopaminergic neurons in striatum can be down-regulated by dietary factors (here, ingestion of acrylamide, a food contaminant created by heating starchy foods). PMID: 25943760
    11. Comparative modeling and small molecule docking indicated that the tropane pharmacophore of RTI 82 was positioned in the central DA active site with an orientation that juxtaposed the aryliodoazide group for cross-linking to rat DAT Phe-319. PMID: 25179220
    12. Data indicate that internalized dopamine transporter (DAT) colocalized with GFP-Rab11 and suggest that Rab11 plays an important role in the recycling of DAT following AMPH-induced DAT down-regulation. PMID: 23612789
    13. Dopamine transporter distribution in the cytoplasm and synaptosomes of substantia nigra in later life is linked to previous environmental toxin exposure. PMID: 23567316
    14. In corpus striatum slices, basal activity of Akt2 regulates DAT cell surface expression. PMID: 22778840
    15. Dopamine transporter loss in Parkinson's disease PMID: 23300642
    16. Both dopamine transporter and VMAT2 are inhibited by HIV-1 Tat protein in corpus striatum synaptosomes. PMID: 22570010
    17. Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding PMID: 23161550
    18. Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. PMID: 22722938
    19. Maternal separation altered the dopaminergic system by decreasing surface expression of dopamine transporter (DAT) and/or the affinity of DAT for dopamine, in the striatum of spontaneously hypertensive rats. PMID: 22133315
    20. Temporal overexpression of LRRK2(G2019S) in adult rats impaired dopamine reuptake by dopamine transporter and thus enhanced locomotor activity. PMID: 21698001
    21. These results indicate a role for the active dopamine transporter in mediating the suppression of TH expression in midbrain dopaminergic neurons by antipsychotic drugs. PMID: 21396352
    22. Data suggest that axonal dopamine transporters (DAT) mainly shuttle between the plasma membrane and recycling endosomes, whereas in the somatodendritic region of neurons DAT traffics through all conventional endosomal pathways. PMID: 20816972
    23. These results reveal a novel role for CPE in the regulation of DAT trafficking and DAT-mediated dopamine uptake. PMID: 19419578
    24. S-palmitoylation as a major regulator of DAT properties that could significantly impact acute and long term dopamine transport capacity. PMID: 21118819
    25. Data support the hypothesis that the dopamine transporter (DAT) adopts an outward facing conformation in a cholesterol-rich membrane environment, suggesting a novel modulatory role of the surrounding membrane lipid milieu on DAT function. PMID: 20688912
    26. Data indicate that selected derivatives showed high in vitro potency and selectivity at SERT, NET, and DAT. PMID: 20170186
    27. These results strongly suggest that DAT glycosylation is involved in the differential vulnerability of midbrain dopamine cells in Parkinson's disease. PMID: 19766189
    28. Data show that the wild-type dopamine transporter displays apparent affinities for substrate efflux more than 300-fold lower than those for uptake. PMID: 12480180
    29. dopamine membrane transporter (DAT) protein density and mRNA expression is upregulated in restricted-fed adult male rats PMID: 12521926
    30. dopamine transporters are a direct substrate for protein phosphatase 1 in vitro and suggest that PP1 is a major DAT phosphatase in rat striatum. PMID: 12573538
    31. dopamine transporter constitutively internalizes and recycles in a protein kinase C-regulated manner in stably transfected PC12 cell lines PMID: 12682063
    32. Observed differences in density and distribution of DAT1 sites in Wistar Kyoto rats may lead to altered modulation of synaptic dopamine levels in cell body and mesolimbic regions, contributing to the depression-like behaviors reported in this rat strain. PMID: 14499307
    33. tyrosine hydroxylase and the dopamine transporter are differentially distributed in the medial- versus the lateral ventral pallidum. PMID: 14681933
    34. the carboxyl terminus regulates dopamine transporter export from endoplasmic reticulum PMID: 15128747
    35. The decrease in DAT cell surface expression in the mPFC may be responsible for decreased DAT function in the mPFC of stimulated compared with deprived rats; there is plasticity in the regulatory mechanisms mediating DAT trafficking and function. PMID: 15935059
    36. This expression profile of the dopamine transporter suggests that dopamine may be taken up not only in the synapses but also extrasynaptically by dopamine transporter, diffusely distributed in both plexiform layers. PMID: 16708013
    37. Cocaine binding near TM6 may overlap the dopamine translocation pathway and function to inhibit TM6 structural rearrangements necessary for transport PMID: 17255098
    38. Lactotroph cells expressing TH coexpressed dopamine transporter and cleaved caspase-3 postlactation. PMID: 17363452
    39. Data show that valproate treatment significantly increases dopamine transporter gene expression in a Sp transcription factor-dependent manner. PMID: 17439486
    40. This is the first evidence showing that DAT can be activated by the firing activity in nigrostriatal cells in a direct way and without the participation of pre-synaptic DA receptors PMID: 17561841
    41. The pharmacological effect of nicotine to increase DAT function and cell surface expression was apparent, such that rats with inherently lower DAT function show a greater sensitivity to the neurochemical effect of nicotine. PMID: 17651428
    42. Relationship between conformational changes in the dopamine transporter and cocaine-like subjective effects of uptake inhibitors is reported. PMID: 17978168
    43. chronic cocaine self-administration followed by abstinence leads to persisting alterations in normal DAT1 trafficking and catalytic regulatory cascades in the CPu and NAcc in a brain region-specific manner. PMID: 18198344
    44. These results directly establish the proximity of transmembrane domains 1 and 6 in DAT and suggest that the mechanism of transport inhibition by cocaine involves close interactions with multiple regions of the substrate permeation pathway. PMID: 18216182
    45. Coinjection of lentiviruses expressing DAT and gamma-synuclein - leading to overexpression of both proteins in the NAc - resulted in a strong increase in cocaine-induced rat locomotor activity suggesting a synergetic role of both proteins. PMID: 18588534
    46. It has been found that GBR12909 is capable of inducing so-called "slow isomerization step" upon binding to DAT, probably consisting of a conformational change in the transporter protein. PMID: 18845198
    47. These data suggest that neuronal activity can regulate dopamine transporter function and abundance via calcium/CaM kinase II signaling. PMID: 19046383
    48. Dopamine transporter over-expression in rats' nucleus accumbens leads to impulsive and risk prone phenotype. PMID: 19135135

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell projection, neuron projection.
  • 蛋白家族：
    Sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family, SLC6A3 subfamily
  • 数据库链接：

    KEGG: rno:24898

    STRING: 10116.ENSRNOP00000047272

    UniGene: Rn.10093