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  4. Recombinant Rat Sphingosine kinase 1 (Sphk1)

Recombinant Rat Sphingosine kinase 1 (Sphk1)

  • 货号：
    CSB-YP022564RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022564RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022564RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022564RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022564RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sphk1; Sphingosine kinase 1; SK 1; SPK 1; EC 2.7.1.91; Acetyltransferase SPHK1; EC 2.3.1.-
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-383
  • 氨基酸序列
    MQPADCPRGL LPRPCRVLVL LNPRGGKGKA LKLFQSRVRP LLEEAEVSFK LMLTERQNHA RELVCAEELG HWDALAVMSG DGLMHEVVNG LMERPDWESA IQKPLCSLPG GSGNALAASL NYYAGHEQVT NEDLLINCTL LLCCRQLSPM NLLSLHTASG RQLYSVLSLS WGFVADVDLE SEKYRSLGEI RFTVGTFFRL ASLRIYQGQL AYLPVGKAAS KIPASSLAQK GPANTYLVPL EEPVPPHWTV VPEQDFVLVL VLLHTHLSTE MFAAPMGRCE AGVMHLFYIR AGVSRAMLVR LFLAMQKGKH MDLDCPYLVH VPVVAFRLEP RNQRGVFSVD GELMVCEAVQ GQVHPNYLWM VSGSSDSPSG RDSQRRPPPE EPI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA.; Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
  • 基因功能参考文献：
    1. Pre-silencing of SphK1 with siRNA reversed PDGF-induced S1P production, miR-21 up-regulation, BMPRII and Id1 down-regulation, as well as pulmonary arterial smooth muscle cell proliferation. PMID: 30453304
    2. Extracellular vesicles from human-induced pluripotent stem cell-derived mesenchymal stromal cells protect against renal ischemia/reperfusion injury via delivering SP1 and transcriptionally activating sphingosine kinase 1 and inhibiting necroptosis. PMID: 29233979
    3. SPHK1 mediated autophagy is different in neurons and neuroblastoma cells PMID: 27467777
    4. In the present study, This study demonstrated that inhibition of Sphk1 decreased IL17 production in microglia after cerebral IR in vivo and in cells cultured under oxygen-glucose deprivation reperfusion conditions. PMID: 28284343
    5. These results indicated that Sphk1/S1P regulates the expression of IL-17A in activated microglia, inducing neuronal apoptosis in cerebral ischemia/reperfusion. PMID: 27002656
    6. data indicated that p53 protein and inhibition of SphKs may be early key events responsible for cell death evoked by Abeta1-42 PMID: 26334640
    7. FTY720 alleviates tubulointerstitium inflammation in an albumin overload rat model of nephropathy via down-regulation of the Sphk1 pathway. PMID: 25399649
    8. the activated SphK1-S1P signaling pathway in glomerular mesangial cells under diabetic conditions is closely associated with AP-1 to form a positive feedback loop. PMID: 24342046
    9. increased Sphk1 expression in the liver in response to endotoxemia mediates the hyperinflammatory state observed in aged animals. PMID: 23817990
    10. Berberine is an important agent against diabetic nephropathy through inhibition of SphK1/AP-1 pathway and suppression of TGFB1/fibronectin synthesis. The inhibition of SphK1/AP-1 by berberine is independent of ambient glucose concentration. PMID: 23085271
    11. Immunohistochemical/pharmacological data suggest that expression of Sphk1 is higher in endothelium/smooth muscle of carotid arteries of spontaneously hypertensive rats than of normotensive rats; Sphk1 is involved in regulation of vascular resistance. PMID: 22251137
    12. the SphK1 pathway plays a critical role in matrix accumulation in glomerular mesangial cells under diabetic condition PMID: 21998146
    13. SphK1 activity is important for insulin synthesis and secretion PMID: 22155656
    14. the SphK1/S1P(3) signaling axis is upregulated when astrocytes are activated by LPS PMID: 21887342
    15. Data suggest that sphingosine kinase 1 expression in amoeboid microglial cells may be linked to regulation of proinflammatory cytokines via an NFkappaB signaling pathway. PMID: 21310085
    16. Glucose-supported beta-cell growth appears to be in part mediated by SPHK activity. PMID: 20371493
    17. Sphingosine kinase 1 is an intracellular effector of phosphatidic acid PMID: 15310762
    18. Data demonstrate the critical role of sphingosine kinase (SPK)1 in lipopolysaccharide signaling in macrophages and suggest that SPK1 is a potential therapeutic target to block hyperimmune responses induced by gram-negative bacteria. PMID: 15314148
    19. Comparison of base sequence of SPHK1/Sphk1 in human, mouse, and rat. PMID: 15585953
    20. results suggest that the SK1/S1P pathway may play an important role in colon carcinogenesis, in part, by regulating COX-2 expression and PGE2 production PMID: 16319132
    21. Exogenous sphingosine 1-phosphate and sphingosine kinase-1 activity regulated myometrial contraction and may be of physiological relevance in regulation of uterine motility during gestation and parturition. PMID: 16956968
    22. These findings suggest that Sphk1 plays a critical role in intestinal tumor cell proliferation and that inhibitors of Sphk1 may be useful in the control of intestinal cancer. PMID: 16980623
    23. Upregulation of ceramide/sphingosine 1-phosphate ratio is a critical event in MAO-A-mediated cardiac cell apoptosis. PMID: 17158340
    24. Uterine expression of SphK1 mediates processes involved in growth and differentiation of uterine tissues during pregnancy. PMID: 17164439
    25. shows that extracellular nucleotides besides being mitogenic also trigger MC migration and this cell response critically requires SK-1 activity PMID: 17200676
    26. SphK-1 and its product S1P are involved in the control of P-gp activity in RBE4 cells. This kinase and its product could contribute to the acquisition and the maintenance of the multidrug resistance phenotype in brain tumor-derived endothelial cells. PMID: 17316399
    27. Cloning and characterization of a novel isoform of rat SK1 (rSK1) with an N-terminal extension (long-rSK1), is reported. PMID: 18028875
    28. SK1 expression does not correlate with enzyme activity. PMID: 18552482
    29. S1P modulates spinal nociceptive processing through inhibition of neuronal cAMP synthesis and sphingosine kinase 1, but not sphingosine kinase 2 PMID: 18805787
    30. Overexpression of SK1, but not SK2, enhanced calcium entry. PMID: 19797403

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane. Endosome membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse.
  • 组织特异性：
    Expressed in microglia (at protein level).
  • 数据库链接：

    KEGG: rno:170897

    STRING: 10116.ENSRNOP00000029296

    UniGene: Rn.18522