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  4. Recombinant Rat Syntaxin-binding protein 1 (Stxbp1)

Recombinant Rat Syntaxin-binding protein 1 (Stxbp1)

  • 中文名称：
    大鼠Stxbp1重组蛋白
  • 货号：
    CSB-YP022905RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Stxbp1重组蛋白
  • 货号：
    CSB-EP022905RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Stxbp1重组蛋白
  • 货号：
    CSB-EP022905RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Stxbp1重组蛋白
  • 货号：
    CSB-BP022905RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Stxbp1重组蛋白
  • 货号：
    CSB-MP022905RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Stxbp1; Unc18a; Syntaxin-binding protein 1; N-Sec1; Protein unc-18 homolog 1; Unc18-1; Protein unc-18 homolog A; Unc-18A; p67; rbSec1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-594
  • 氨基酸序列
    MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI MTEGITIVED INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR AAHVFFTDSC PDALFNELVK SRAAKVIKTL TEINIAFLPY ESQVYSLDSA DSFQSFYSPH KAQMKNPILE RLAEQIATLC ATLKEYPAVR YRGEYKDNAL LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS SPVLHELTFQ AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL AEDCMKHYQG TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS TYDKIRIILL YIFLKNGITE ENLNKLIQHA QIPPEDSEII TNMAHLGVPI VTDSTLRRRS KPERKERISE QTYQLSRWTP IIKDIMEDTI EDKLDTKHYP YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF ILGGVSLNEM RCAYEVTQAN GKWEVLIGST HILTPQKLLD TLKKLNKTDE EISS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
  • 基因功能参考文献：
    1. Together with previous studies, these data support a model whereby Munc18-1 acts as a template for SNARE complex assembly, and autoinhibition of synaptobrevin binding contributes to enabling regulation of neurotransmitter release by Munc13-1. PMID: 28477408
    2. Munc18a promotes membrane clustering in a step that is independent of trans-SNARE activation. PMID: 28827281
    3. The ternary complex of syntaxin1:SNAP25:Munc18-1binds synaptobrevin with fast kinetics, resulting in the rapid formation of a fully zippered SNARE complex to which Munc18-1 remains tethered by the N-terminal domain of syntaxin1. PMID: 28483813
    4. Munc18-1 mutations and haploinsufficiency may therefore trigger a pathogenic gain of function through both the corruption of native Munc18-1 and a perturbed chaperone activity for alpha-Syn leading to aggregation-induced neurodegeneration. PMID: 27597756
    5. TNFalpha induces co-trafficking of TRPV1/TRPA1 in VAMP1-containing vesicles to the plasmalemma via Munc18-1/syntaxin1/SNAP-25 mediated fusion. PMID: 26888187
    6. Data demonstrate that the open-closed syntaxin equilibrium is shifted toward the open state when syntaxin and Munc18-1 are associated with SNAP25, and the results indicate that a syntaxin/SNAP25:Munc18-1 complex is a likely starting point for SNARE assembly. PMID: 26876096
    7. Measuring rapid interaction dynamics during synaptic depolarization identified the small number of syntaxin1a and munc18-1 protein molecules required to support synaptic vesicle exocytosis. PMID: 25517944
    8. Secretory vesicles dock by inducing syntaxin1/munc18 clustering in the target membrane. PMID: 24835618
    9. Munc18a does not alter synaptic fusion rates mediated by neuronal SNAREs, synaptotagmin-1, and complexin-1. PMID: 25716318
    10. Synaptotagmin-1 is an antagonist for Munc18-1 in SNARE zippering. PMID: 25716321
    11. SEC1/Munc18-SNARE interactions at other transport steps may provide a general mechanism to drive lipid bilayer merger. PMID: 24532794
    12. PTPRT could regulate the interaction of Syntaxin-binding protein 1 with Syntaxin 1, and as a result, the synaptic vesicle fusion appears to be controlled through dephosphorylation of Syntaxin-binding protein 1 PMID: 23962429
    13. the priming mechanism of Munc18-1 involves its domain-3a-dependent interaction with the SNARE complex. PMID: 23525015
    14. a defined role for Munc18-1 in facilitating exocytosis linked to the loop region of domain 3a that is clearly distinct from its function in Sx1a transport. PMID: 23761923
    15. Double knockdown of Munc18-1 and Munc18-2 in mast cells eliminates both IgE-dependent and ionomycin-induced degranulation and causes a significant reduction in syntaxin-11 without altering expressions of the other syntaxin isoforms examined. PMID: 23487749
    16. Munc18-1 molecules move between membrane depots distinct from vesicle morphological docking sites PMID: 23223447
    17. it is proposed that fusion does not proceed through syntaxin-1-SNAP-25 heterodimers but starts with the syntaxin-1-Munc18-1 complex; Munc18-1 and Munc13 then orchestrate membrane fusion together with the SNAREs and synaptotagmin-1-Ca(2+) PMID: 23258414
    18. SNAREpin assembly by Munc18-1 requires previous vesicle docking by synaptotagmin 1. PMID: 22810233
    19. Munc18 can control and assist in SNARE-complex assembly during neurotransmitter release PMID: 21900493
    20. the domain-1 cleft of Munc18-1 is essential for binding to syntaxin-1 and thereby critical for its chaperoning, docking, and secretory functions PMID: 21900502
    21. Munc18-1 acts downstream of Munc13-1/RIM/Rab3A and interacts with syntaxin 1a allowing vesicle priming. Munc18-1 promotes Rab3A dissociation from vesicles, which then results in fusion. PMID: 21689256
    22. This suggests that Munc18a controls the accessibility of syntaxin 1a to its partners, a role that might be common to all Sec1/Munc18-like proteins. PMID: 18337752
    23. Munc18-1 and the neuronal SNAREs (t-SNARE (syntaxin 1.SNAP-25) and v-/t-SNARE (VAMP2.syntaxin 1.SNAP-25) complexes) already have the inherent capability to function as a basic stage-specific off/on switch to control membrane fusion PMID: 21730064
    24. results suggest that excitatory stimulation can induce the distribution change of Munc18-1 in neuron, which may subsequently modulate neuronal functions in brain. PMID: 21614099
    25. Munc18-1 allows for the formation of a complex between syntaxin and SNAP-25 that serves as an acceptor for vesicle-bound synaptobrevin PMID: 17002520
    26. The results of molecular dynamics simulations suggest that the free wild-type munc18a exhibits a dynamic equilibrium between several conformations differing in the size of its cavity (the main syntaxin-binding site). PMID: 21390273
    27. Data show that a phosphorylation site in syntaxin 1 (Ser(14)) regulates the N-terminal interaction with Munc18-1. PMID: 19748891
    28. Results indicate that Munc18-1 and -2 act to support the expression of syntaxins and to deliver syntaxin-1 to the plasma membrane, and that the binding to the closed conformation of syntaxin is essential for Munc18-1 stimulatory action. PMID: 19812250
    29. Since Munc-18a can bind both isoforms of syntaxin 1, the present data suggest that this protein is a general modulator of the formation of different SNARE complexes in the nerve endings. PMID: 12093152
    30. nSec1/Munc18a is involved in the regulation of SNARE complex-dependent membrane fusion events implicated in the ramification of axonal processes in hippocampal neurons. PMID: 12182895
    31. the biochemical evidence of strong interaction between Munc18-1 with cytoskeletal proteins PMID: 12963086
    32. Munc-18-1 is a potent negative regulator of basal phospholipase D activity PMID: 14744865
    33. Syntaxin/Munc18 interactions in the late events during vesicle fusion and release in exocytosis PMID: 15175344
    34. Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. PMID: 15563604
    35. The presence of both nuclear import and export signals in Munc18-1 primary structure corroborates its nuclear localization and makes it a putative shuttle protein between nuclear and cytoplasmic compartments. PMID: 15869823
    36. Munc18-1, expressed in an estrogen-dependent manner, plays an important role in the onset of female puberty via the regulation of glutamate release. PMID: 16951547
    37. Munc18-1 accelerates fusion only for the cognate SNAREs for exocytosis, therefore enhancing fusion specificity. PMID: 17218264
    38. Up-regulated ny estradiol. PMID: 17293448
    39. Munc18-1 also binds tightly to assembled SNARE complexes containing syntaxin-1. PMID: 17301226
    40. We propose that Munc18-1 inhibitis the SNARE complex assembly by stabilizing Syntaxin1A in a closed conformation in vesicle priming process, therefore negatively regulates insulin secretion. PMID: 17617378
    41. Munc18-1 plays a critical role for the plasma membrane localization of syntaxin1 in neuroendocrine cells and therefore necessitates the interpretation of Munc18-1 mutant phenotypes to be in terms of mislocalized syntaxin1. PMID: 18077557
    42. Data suggest that Munc18a binds to the Syntaxin1a NRD and H3 domain within the assembled t-SNARE complex, positioning them for productive VAMP2 binding, so that Munc18a facilitates efficient SNARE-mediated membrane fusion. PMID: 18829865
    43. Immunohistochemical analysis revealed that the protein level of syntaxin1 was markedly up-regulated in the ischemic areas where immunoreaction for MAP2 was lost. PMID: 19344701
    44. The Munc18-1 hydrophobic pocket is therefore critical for SNARE complex binding PMID: 19483085
    45. In primary cultured hippocampal neurons, a role is suggested for schizophrenia-related dysbindin in synaptic vesicle exocytosis via interaction with Munc18-1. PMID: 19573021
    46. show that presynaptic metabotropic glutamate receptors (mGluRs) bind and release Munc18-1 in a Ca(2+)-dependent manner, whose actions decrease and increase synaptic vesicle release, respectively. PMID: 19822743

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  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    STXBP/unc-18/SEC1 family
  • 组织特异性：
    Brain and spinal cord. Highly enriched in axons.
  • 数据库链接：

    KEGG: rno:25558

    STRING: 10116.ENSRNOP00000021189

    UniGene: Rn.80843