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  4. Recombinant Rat Transcription factor GATA-4 (Gata4)

Recombinant Rat Transcription factor GATA-4 (Gata4)

  • 中文名称：
    大鼠Gata4重组蛋白
  • 货号：
    CSB-YP009277RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Gata4重组蛋白
  • 货号：
    CSB-EP009277RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Gata4重组蛋白
  • 货号：
    CSB-EP009277RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Gata4重组蛋白
  • 货号：
    CSB-BP009277RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Gata4重组蛋白
  • 货号：
    CSB-MP009277RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gata4Transcription factor GATA-4; DNA-binding protein GATA-GT2; GATA-binding factor 4
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-440
  • 氨基酸序列
    MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG LSYLQGGGSG AASGATSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYSSSGGAAG AGLAGREQYG RPGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPAR HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC GLYHKMNGIN RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPPGESLPP SSGASSNSSN ATSSSSSSEE MRPIKTEPGL SSHYGHSSSM SQTFSTVSGH GSSIHPVLSA LKLSPQGYPS PVTQTSQASS KQDSWNSLVL ADSHGDIITA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase.
  • 基因功能参考文献：
    1. Bioinformatics analysis and the dual-luciferase reporter assay demonstrated that miR-200c directly targeted the 3'-untranslated region of GATA-4, an important transcription factor for cardiomyocyte survival. PMID: 28440427
    2. downregulation of miR-122 alleviates cardiomyocyte hypoxia/reoxygenation injury through upregulation of GATA-4 expression. PMID: 27569279
    3. The inhibition of LOX-1 by RNA interference LOX-1 lentivirus resulted in the loss of PI3K/Akt activation and GATA4 protein expression. GATA4 may play an important role in the LOX-1 and PI3K/Akt regulation of cardiac fibroblast proliferation. PMID: 27216460
    4. the present study has identified a protective role of miR-26a via suppression of GATA4, and, in turn, further downstream factors in cardiac hypertrophy. PMID: 27485101
    5. This study demonstrates the protective function of miR-208b via GATA4 in post-infarction myocardial fibrosis. Luciferase reporter assay proved Gata4 to be the direct target of miR-208b, with the target sequence in the 3'UTR. PMID: 27236543
    6. exogenous expression of cardiac-specific transcription factors GATA4 and TBX5 enhance cardiomyogenic differentiation bone marrow mesenchymal stromal cells PMID: 26100648
    7. Clusterin/Akt signaling pathway is involved in GATA-4 mediated cytoprotection against hypoxia stress. PMID: 26962868
    8. The expression and transcriptional activity of GATA4 in cardiomyocytes is inhibited by TIEG1 expression. PMID: 26252173
    9. The role of ZAK and/or the ZAK downstream events such as JNK and p38 phosphorylation, c-Jun, and GATA-4 nuclear translocation in cardiac hypertrophy. PMID: 25869677
    10. Silencing of GATA-4 expression in cultured intestinal epithelial cells identified Reg3beta as a target gene using an unbiased approach of gene expression profiling. PMID: 26477491
    11. Exo(GATA-4) upon transplantation in the damaged tissue mediate protection by releasing multiple miRs responsible for activation of the cell survival signaling pathway. PMID: 25590961
    12. These results establish CDK4 as a GATA4 partner in cardiogenesis PMID: 25241353
    13. Mesenchymal stem cells (MSC)/(GATA-4) protects cardiomyocytes (CM) under hypoxic conditions. The release of IGF-1 and VEGF from MSC(GATA-4) is likely to be responsible for protection of CMs. PMID: 25000170
    14. Histone acetylation is essential for the regulation of GATA4 by bone morphogenetic protein (BMP)2 in cardiac myocytes. PMID: 23632743
    15. Cytokine (VGEF and IGF-1) release from GATA-4 overexpressing MSC can partially account for the upregulated ion channel mRNA expression PMID: 24582939
    16. Cardiomyocyte protection by GATA-4 gene engineered mesenchymal stem cells is partially mediated by translocation of miR-221 in microvesicles. PMID: 24015301
    17. In experimental diabetes, there was an increase of expression of GATA-4 in the heart. PMID: 22293779
    18. Diaphragmatic expression of GATA4 is downregulated in nitrofen-induced congenital diaphragmatic hernia. PMID: 23426975
    19. new insight into GATA4 mechanisms of action: a distinct regulatory pathways regulate activities of GATA4 in embryonic development and postnatal hearts PMID: 22473995
    20. These results indicated that EPO-induced ERK signaling activation increased GATA-4 phosphorylation and acetylation and these processes required the phosphorylation of GATA-4 at Ser-261 residue. PMID: 22674427
    21. GATA4 is activated by the IGF1 pathway, but although it is required for responses to pathological stimuli, it is not necessary for the effects of IGF1 on cardiac structure and function. PMID: 22228770
    22. Hyperglycemia can cause systolic dysfunction and a higher expression of cTnI in cardiomyocytes through ROS, enhancing MEK/ERK-induced GATA-4 phosphorylation and accumulation in the cell nucleus. PMID: 21702924
    23. promotes myocardial transdifferentiation of mesenchymal stromal cells via up-regulating IGFBP-4 PMID: 21846294
    24. GATA4 stimulates transcriptional activity of the luteinizing hormone beta gene promoter PMID: 21571865
    25. Activation of the promoter of miR-144/451 by GATA-4, a critical transcription factor in the heart, was identified. PMID: 20708014
    26. These results establish a right heart-specific signaling mechanism in response to pressure overload, which involves metal-catalyzed carbonylation and degradation of annexin A1 that liberates CBF/NF-Y to activate Gata4 gene transcription. PMID: 21059997
    27. Cdk9 forms a functional complex with the p300/GATA4 and is required for p300/GATA4- transcriptional pathway during cardiomyocyte hypertrophy PMID: 20081228
    28. The 5' region of the mouse, rat, and human GATA4 genes, was characterized. PMID: 20041118
    29. GATA-4 and Nkx2. 5 gene expression increased gradually in the course of mesenchymal stem cells differentiation into cardiomyocytes. PMID: 19253817
    30. The ability of GATA4 to inhibit autophagy as a potential mechanism underlying its protection against doxorubicin toxicity in cultured neonatal rat cardiomyocytes, was investigated. PMID: 19901028
    31. the Rho/ROCK pathway is linked to downstream GATA-4 via the activation of extracellular signal-regulated kinases during myocardial cell hypertrophy PMID: 11739382
    32. Direct left ventricular wall stretch activates GATA4 binding in perfused rat heart: involvement of autocrine/paracrine pathways. PMID: 11810204
    33. interacts with dHAND to synergistically activate cardiac gene expression through a p300-dependent mechanism PMID: 11994297
    34. Retinoid X receptor alpha represses this protein's transcription via a retinoid-dependent interaction with the cardiac-enriched repressor FOG-2. PMID: 12480945
    35. GATA-4 is a novel downstream effector of cAMP/PKA signaling in gonadal cells PMID: 12670947
    36. activation of GATA-4, in cooperation with a factor binding on Nkx-2.5 binding element, is essential for mechanical stretch-induced cardiomyocyte hypertrophy PMID: 12704188
    37. GATA-4 binding in intact heart requires both p38 and extracellular signal-regulated protein kinases PMID: 15051723
    38. interaction of GATA-4 with a transcriptional coactivator p300 is required for its full transcriptional activity and the activation of the embryonic program during myocardial cell hypertrophy PMID: 15220332
    39. The study shows that d-myo-inositol 1,4,5-tris-phosphate-induced cardiac hypertrophy is mediated by GATA4 but independent from the calcineurin pathway. PMID: 16259952
    40. GATA-4 and CCAAT/enhancer-binding protein beta (C/EBPbeta) bind to the Star promoter and activate transcription PMID: 16682116
    41. Delivery of GATA4 locally to the myocardial infarction border zone induces multiple local effects in the border zone cardiac myocytes resulting in beneficial ventricular remodeling and improved global left ventricular function. PMID: 17495229
    42. Gata4 regulates expression of Ca2+ channels in neonatal heart. PMID: 17498735
    43. Increased GATA4 protein degradation may be an important mechanism that contributes to hyperglycemic cardiotoxicity. PMID: 17525155
    44. the acetylation of GATA4 as one of critical nuclear events in myocardial cell hypertrophy. PMID: 18252717
    45. Episodic gonadotropin-releasing hormone (GnRH) gene expression pulses are mediated by GATA-4, likely acting through GATA-binding sites in the GnRH neuron-specific enhancer region. PMID: 18259093
    46. Fibroblasts and a proportion of pericytes in the adult rat heart express the transcription factor GATA4. PMID: 19162035
    47. Atrial natriuretic peptide suppresses endothelin gene expression and proliferation in cardiac fibroblasts through a GATA4-dependent mechanism. PMID: 19546173

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed predominantly in gastric mucosa and at much lower levels in the intestine. Also expressed in testis.
  • 数据库链接：

    KEGG: rno:54254

    STRING: 10116.ENSRNOP00000014320

    UniGene: Rn.26251