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  4. Recombinant Rat Transforming growth factor beta-1 proprotein (Tgfb1), partial

Recombinant Rat Transforming growth factor beta-1 proprotein (Tgfb1), partial

In Stock
  • 货号：
    CSB-EP023446RA
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tgfb1; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-1; TGF-beta-1)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    44.5kDa
  • 表达区域：
    30-278aa
  • 氨基酸序列
    LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDIREAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.; Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia. Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.; Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
  • 基因功能参考文献：
    1. High TGFB1 expression is associated with glomerular mesangial cell hypertrophy. PMID: 29397070
    2. High TGFB1 expression is associated with liver fibrosis. PMID: 30243650
    3. In the present study, we assessed the effect of boldine on the progression of kidney disease using the 2K1C hypertension model and identifying mediators in the RAS, such as TGF-β, that could be modulated by this alkaloid PMID: 29941815
    4. Ang II induced epithelial-mesenchymal transition in renal tubular epithelial cells via Tgfb1. PMID: 29746862
    5. LET suppression promoted BMSCs proliferation by up-regulating the expression of TGF-beta1 and activating Wnt/beta-catenin pathway. PMID: 29068476
    6. These findings implicate TGFbeta1 as one of the important factors impairing drug induced CYP induction in co-cultures and suggests that caution needs to be exercised in the use of hepatocyte-fibroblast co-cultures for CYP induction studies. PMID: 29366910
    7. This suggests that MG53 is a potential regulator of atrial fibrosis induced by the TGF-beta1/Smad pathway in patients with atrial fibrillation . PMID: 29233682
    8. This suggests that soybean isoflavones could delay the process of renal interstitial fibrosis in DN rats by decreasing the expression of Wnt4, beta-catenin and TGF-beta1 in the renal interstitium, thus demonstrating that soybean isoflavones plus losartan have the best protective effects against diabetes-induced renal fibrosis. PMID: 29921370
    9. The results of our study suggest that miR-142 inhibits proliferation and promotes apoptosis in ASMCs during airway remodeling in asthmatic rats by inhibiting TGF-beta expression via a mechanism involving the EGFR signaling pathway. PMID: 29949788
    10. In cultured hippocampal neurons, TGF-[beta]1 prevented A[beta]1-42-induced APP, TNF-[alpha], Bax, cleaved caspase-3, and cleaved caspase-9 expression upregulation, and cell apoptosis, showing a neuroprotective effect of TGF-[beta]1. Silencing of the T[beta]R1 gene abolished the neuroprotective effect of TGF-[beta]1. PMID: 29200096
    11. Acetyl-11-Keto-beta-Boswellic Acid is able to attenuate oxidative stress and profibrotic mechanisms, and improve vascular remodeling in hypertension through TGF-beta1/Smad3 pathway. PMID: 28009003
    12. the truncated variant of gHGF (a double mutant of N-terminal hairpin and first kringle domains of gHGF, K132E and G134E, gmNK1) protein fused with His6 tag, the molecular weight of which was about 20.0kDa, which was expressed in Escherichia coli BL21 (DE3) and purified with Ni(2+)-affinity chromatography.gmNK1 inhibited protein expression levels of fibrosis-related Col I and alpha-SMA in TGF-beta1-activated HSC-T6 cells PMID: 28837849
    13. The results suggest that the CIP4/Par6 complex plays an important role in the occurrence of epithelial-mesenchymal transition in TGF-beta1-stimulated epithelial kidney cells. PMID: 28848997
    14. calcitonin and TGF-beta have roles in cartilage degeneration and nociception in an experimental rat model of osteoarthritis PMID: 27345362
    15. Suggest a potentially mutual regulation between IGFBPrP1 and TGFbeta1, which activates hepatic stellate cells and extracellular matrix deposition. PMID: 28823370
    16. Study demonstrated that the activation of transforming growth factor-beta1 (TGF-beta1) in mild traumatic brain injury (mTBI) correlated with the induction of free radical generating enzyme NADPH oxidase 1 (NOX1). Study study could establish the significance of TGF-beta1 in transforming the pathophysiology of mTBI into secondary injury. PMID: 28433746
    17. TGF-Beta1 (and Egr-1) are up-regulated by sustained hyperosmolarity in the renal medulla. PMID: 28673338
    18. The positive correlation between Reg1a and Tgfb1 genes expression was determined in the dynamics of stress-induced gastric lesions development and healing, which may indicate the involvement of Tgfb1 in acceleration of lesions healing. PMID: 29537200
    19. In conclusion, PFD and Collagen-PVP decrease inflammation, fibrosis, TGFbeta-1 expression, and CD in the posttracheoplasty rats' scar. PMID: 28584818
    20. TGFB1-expressing mesenchymal stem cells induce local tolerance in a rat liver transplantation model of acute rejection. PMID: 27333806
    21. In the present study, isolated rat peritoneal mesothelial cells were treated with TGF-beta1 and/or IL-6 which elicited mesenchymal transition (MMT) as determined by morphologic and biochemical techniques ..Partial characterization of relevant signaling pathways identified Erk1/2 activation, which was enhanced by combined TGF-b1/IL-6 administration, as a crucial necessary factor in the transition.. PMID: 26825317
    22. miR-497 could suppress TGFbeta1-induced pulmonary fibroblast proliferation and EIF3A, Collagen I and alpha-SMA protein levels. PMID: 28322466
    23. could prevent cardiac fibroblasts from activation into myofibroblast and protect HUVEC against endothelial to mesenchymal transition. These effects may be mediated by inhibition of the TGFbeta pathway in both cardiac fibroblasts and HUVECs. PMID: 28303407
    24. Administration of Methylglyoxal led to depletion of antioxidant enzymes, induction of fibrosis (p < 0.001), up-regulated expression of RAGE (3.5 fold), TGF-beta (4.4 fold), SMAD2 (3.7 fold), SMAD3 (6.0 fold), IL-6 (4.3 fold) and TNF-alpha (5.5 fold) in the heart tissues compared to control rats. PMID: 28091942
    25. High TGFB1 expression is associated with pulmonary arterial hypertension. PMID: 29069575
    26. Data suggest that white adipose tissues in various sites respond differently to distinct diet patterns regarding Tgfb expression and signaling pathways; here, energy-restricted and fat-restricted diet containing fish oil down-regulates expression of Tgfb in mesenteric adipose tissue and up-regulates expression of Tgfb in retroperitoneal adipose tissue. PMID: 27736730
    27. Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-beta-mediated Nox4 expression. PMID: 28063381
    28. Transforming growth factor beta 1 overexpression observably activated these two pathways, suggesting that Transforming growth factor beta 1 might participate in the modulation of vitamin D through regulating ERK and JNK pathways in Bone marrow-derived mesenchymal stem cells. PMID: 28817810
    29. The data suggest that miR-155 plays a role in mediating TGF-beta1-induced podocyte injury via nephrin, desmin and caspase-9. PMID: 28719898
    30. This paper provides evidence that TGF-beta stimulates prolonged EGFR transactivation, which is dependent on c-Src tyrosine kinase (Src). PMID: 28943431
    31. Although TGF-beta promotes circulating regulatory T cells (Tregs)in female spontaneously hypertensive rats (SHR), it does not account for the sex difference in renal Tregs in SHR. PMID: 28679591
    32. Mesenchymal stem cells could inhibit the process of chronic rejection of small intestine transplant. The mechanisms may include the inhibited function of these cells on lymphocyte proliferation, reduced infiltration of macrophages, and reduced expression of transforming growth factor beta1 and platelet-derived growth factor C. PMID: 28338461
    33. this study shows that FTY720 attenuates renal fibrosis by TGF-beta signaling in fibroblasts PMID: 28658504
    34. Apigenin ameliorated renal damage due to diabetic nephropathy by suppressing oxidative stress and fibrosis via MAPK-NF-kappaB-TNF-alpha and TGF-beta1-MAPK-fibronectin pathways. PMID: 28566504
    35. High glucose stress suppresses SnoN while activating TGF-beta1 and Rnf111 (Arkadia) in renal tubular epithelial cells and diabetic rats. PMID: 28350874
    36. The fibromodulin (Fm)-transforming growth factor (TGF)-beta1 nexus plays a crucial in fetal-type scarless skin repair. PMID: 27665369
    37. mRNA/protein levels are increased in kidney remnants from 5/6 nephrectomized rats and strongly correlate with TGF-beta1 expression. PMID: 27714812
    38. Data indicate transforming growth factor beta (TGFbeta) involvement in early activation of platelet-derived growth factor receptor beta (PDGFRbeta)+ pericytes in aristolochic acid induced acute kidney injury. PMID: 27379382
    39. TGFbeta1 is involved in cholangiocarcinoma tumor progression and participates through miR-34a mediated downstream signaling cascades. PMID: 27364974
    40. In high glucose-treated glomerular mesangial cells, TGR5 can suppress the NF-kappaB-mediated upregulation of fibronectin and TGF-beta1 by inhibiting RhoA/ROCK signaling via protein kinase A. PMID: 27470217
    41. this study shows that TGF-beta1 vaccine attenuated diabetic nephropathy in OLETF rats through reduction of inflammation, improvement of kidney fibrosis and partial correction of glucose metabolism PMID: 27485077
    42. No differences in fiber arrangement, structure, and nuclei form were noted in either group (P > 0.05). Our findings suggest that TGF-beta1 signaling is necessary for the regulation of tendon cross-link formation, as well as collagen and LOX gene transcription in an exercise-dependent manner. PMID: 27927626
    43. Overexpression of IL-6 is sufficient to induce myofibroblastic proliferation, differentiation, and fibrosis, probably via increased TGF-beta1-mediated MMP2/MMP3 signaling during ischemic myocardial remodeling monoculture or coculture models under hypoxic conditions. PMID: 27348628
    44. TGFbeta1 specifically elevates the expression level of TRPC6 mainly through Smad-dependent canonical pathway. The siRNA against TRPC6 abolished TGFbeta1-induced molecular and cellular phenotype changes, including myosin light chain phosphorylation, actin stress fiber formation, and cell migration. PMID: 28039055
    45. in diabetes, retinal vessels become dependent on a small increase in TGF-beta signaling via activin receptor-like kinase 5 to maintain early integrity. PMID: 28162229
    46. the application of TSLP short hairpin RNA caused a stark reversal of the TGF-beta1-induced cellular fibrosis. PMID: 27492484
    47. this study shows that adipose-derived stem cells treatment attenuates renal interstitial fibrosis possibly through inhibition of epithelial-mesenchymal transition and inflammatory response via TGF-beta1 signaling pathway PMID: 28092863
    48. Alloxan-induced renal damage is associated with alterations of p53, TGF-beta1, and extracellular matrix metalloproteinases. PMID: 28220578
    49. Calcitriol may protect diabetic nephropathy from fibrosis via inhibition of TGF-beta1 and CIP4. PMID: 26097534
    50. The in vitro and in vivo models revealed that lipid metabolism disorder and FK506 caused oxidative stress and a fibrogenic response. In addition, decreased levels of LOX1 markedly reduced the levels of TGFb1 in the in vitro model. PMID: 27633115

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  • 亚细胞定位：
    [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted.
  • 蛋白家族：
    TGF-beta family
  • 组织特异性：
    Abundant in the bone matrix.
  • 数据库链接：

    KEGG: rno:59086

    STRING: 10116.ENSRNOP00000028051

    UniGene: Rn.40136