Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Tropomyosin alpha-1 chain (Tpm1)

Recombinant Rat Tropomyosin alpha-1 chain (Tpm1)

  • 货号：
    CSB-YP024104RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP024104RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP024104RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP024104RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP024104RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tpm1; Alpha-tm; Tpma; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-284
  • 氨基酸序列
    MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL KGTEDELDKY SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA DESERGMKVI ESRAQKDEEK MEIQEIQLKE AKHIAEDADR KYEEVARKLV IIESDLERAE ERAELSEGKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLSD KLKEAETRAE FAERSVTKLE KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
  • 基因功能参考文献：
    1. Transmission of stability information through the N-domain of tropomyosin is interrupted by a stabilizing mutation (A109L) in the hydrophobic core of the stability control region (residues 97-118). PMID: 24362038
    2. the link between flexibility of TM and its function in ejecting hearts. PMID: 23609439
    3. analysis of the pattern of of evolutionarily conserved basic and acidic residues that constitutes the binding interface of actin-tropomyosin PMID: 23420843
    4. The evolutionarily conserved residues are important for actomyosin regulation, a universal function of Tm that has a common structural basis and mechanism. PMID: 23091026
    5. troponin C (cTnC) and tropomyosin (cTm) are not only present in myofilaments of ventricular cardiomyocytes in culture but are also within their nuclei PMID: 22364878
    6. TPM1 may play an important role in the occurrence and development of liver fibrosis. PMID: 22433308
    7. The results were consistent with a mechanism in which the alpha-tropomyosin-actin interaction cooperatively affects actin to result in the generation of greater force and velocity. PMID: 22041451
    8. Data show that dual expression of two CM mutants, tropomyosin mutant A63V and cardiac troponin mutant R146G, were shown to additively slow myocyte relaxation beyond either mutant studied in isolation. PMID: 20161772
    9. A splicing silencer that regulates smooth muscle specific alternative splicing is active in multiple cell types. PMID: 12177296
    10. The stability and energetics of the tropomyosin coiled coil are important for actin affinity. PMID: 14640678
    11. analysis of tropomyosin bending and binding sites for actin PMID: 16365313
    12. Tm affects the conformation of actin so as to increase the area of hydrophobic interaction between actin and myosin molecules PMID: 16980359
    13. Results report the solution NMR structure of an overlap complex formed of model tropomyosin peptides. PMID: 16999976
    14. generic (interface instability) and specific periodic surface residues are essential for function PMID: 18052203
    15. The data suggest that end-to-end interactions of adjacent Tm molecules are strengthened when Tm is phosphorylated. PMID: 18985725
    16. A nonsense exon in the Tpm1 gene is silenced by hnRNP H and F PMID: 19037011

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Tropomyosin family
  • 数据库链接：

    KEGG: rno:24851

    UniGene: Rn.87540