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  4. Recombinant Rat Urocortin (Ucn)

Recombinant Rat Urocortin (Ucn)

  • 货号：
    CSB-YP025551RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025551RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025551RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025551RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025551RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ucn
  • Uniprot No.：
  • 别名：
    Ucn; Urocortin; Corticotensin
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    81-120
  • 氨基酸序列
    DDPPLSIDLT FHLLRTLLEL ARTQSQRERA EQNRIIFDSV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Plays a role in the establishment of normal hearing thresholds. Reduces food intake and regulates ghrelin levels in gastric body and plasma.
  • 基因功能参考文献：
    1. Study found reduced miR-326 levels concomitant with elevated Ucn1 levels in Edinger-Westphal nucleus (EWcp) of depressed suicide completers and in the EWcp of depressed rats. In fully recovered rats, both serum and EWcp miR-326 levels rebounded to nonstressed levels. While downregulation of miR-326 levels in primary midbrain neurons enhanced Ucn1 expression levels, miR-326 overexpression selectively reduced its levels. PMID: 27045550
    2. urocortin may play a cytoprotective role in the germ cells in response to ischemia-reperfusion injury through the activation of major anti-apoptotic proteins, as well as by the mitogen-activated protein kinase signaling pathway activation. PMID: 27659706
    3. Results suggest that sex differences exist in the distribution of UCN-1 within brainstem autonomic areas, and circulating level of estrogen may play an important role with regards to the function of these UCN-1 neurons during stress responses PMID: 26146233
    4. UCN might contribute to regulate late midbrain dopaminergic neuron differentiation during ventral midbrain development. PMID: 25641682
    5. UCN, CRHR1 and CRHR2 are expressed in the rat epididymis. It is suggested that CRH-related peptides might play multiple roles in the maturation and storage of spermatozoa. PMID: 24856473
    6. Urocortin-1 expressing premotor neurons are located within the Edinger-Westphal nucleus. PMID: 23707488
    7. Ucn1 is a contributory factor to the gender disparity in vasculitis and dihydrotestosterone-promoted Ucn1 secretion exacerbated the development of vasculitis. PMID: 24064363
    8. Ucn1 induces a CRF2 mediated increase in circulating ghrelin via indirect actions on gastric ghrelin cells that involves a nicotinic pathway independently from the hyperglycemic response. PMID: 23183626
    9. The two hypertrophic peptides urotensin II and urocortin stimulate the expression of myostatin. PMID: 22244812
    10. Urocortin derived from dopaminergic neurons is involved in an autocrine protective signaling mechanism. PMID: 19875195
    11. No effects of stress on corticotropin releasing hormone (CRF) and Ucn1 contents are seen in wild-type rat brain, but in Brattleboro rats, stress increases CRF contents in the paraventricular nucleus. PMID: 21621194
    12. Acute pain stress resulted in the activation of urocortin-immunoreactive neurons in the Einger-Wesphal nucleus, peaking at 4 h after acute pain stress. PMID: 22432129
    13. CRF(1) agonists, Ucn 1 and stressin(1) -A, reduced feeding and induced interoceptive stress, whereas Ucn 2 potently suppressed feeding via a CRF(2) -dependent mechanism without eliciting malaise PMID: 21627635
    14. UcnI alone suppresses appetite and promotes fat oxidation and reliably suppresses food intake. PMID: 21303672
    15. Ucn mRNA levels fell below basal levels in association with an increase in the number of Ucn1 immunoreactive inflammatory cells PMID: 21330446
    16. Ucn 1 mRNA expression was higher in the restraint group than in the foot shock group. PMID: 20833218
    17. Changes of somatic angiotensin-converting enzyme activity and production of other renin-angiotensin system components may play roles in the vasodilatory property of Ucn via activation of the extracellular signal-regulated kinase 1/2 pathway. PMID: 20237592
    18. The rsults of this study proposed that the rate of axonal Ucn1 transport and, possibly, the strength of Ucn1 secretion, are dependent on sex to the same degree as is Ucn1 biosynthesis. PMID: 19833156
    19. Ucn1 exerts pro-inflammatory effects by augmenting LPS-induced expression of COX-2 and ICAM-1 in RAECs via CRF(2) receptors and the activation of p38MAPK and NF-kappaB. PMID: 19694731
    20. Data show that Ucn1's effect on L(p) is partially mediated by the CRH-R(2) receptor and acts independently of the c-JUN N-terminal kinase signal transduction pathway. PMID: 19819946
    21. the inhibition of somatodendritic dopamine release by urocortin may be one of the molecular mechanisms underlying the effect of stress on motor function. PMID: 19729048
    22. Ucn may play a key role in the response of the cardiovascular system to stress and a potent suppressor of appetite/feeding behaviour. PMID: 12007627
    23. urocortin has a role in hypertrophy of rat cardiomyocytes as measured by an increase in cell area and enhanced protein: DNA ratio PMID: 12027405
    24. The modulatory effects of the CRF-related peptide urocortin on memory and learning are mediated via CRF1 receptors. PMID: 12376180
    25. urocortin enjoys a close ligand-receptor symmetry in the cerebellum, probably to a greater degree than corticotropin-releasing factor since corticotropin-releasing factor itself is found exclusively in the two major cerebellar afferent systems. PMID: 12379245
    26. both Ucn and Ucn II induced accumulation of intracellular cAMP via corticotropin-releasing factor receptor type 2beta and also caused a significant increase in IL-6 output levels. PMID: 12746280
    27. Urocortin is not only involved with the processing of sensory information conveyed within cerebellar afferents, but also with signals leaving the cerebellar cortex via Purkinje cells. PMID: 15024751
    28. These findings suggest an interaction between Urocortin and NO signaling within the supraoptic nucleus, rather than the paraventricular, that may modulate the regulation of feeding, reproduction, and anxiety-related behaviors. PMID: 15457505
    29. Results suggest that the urocortin gene locus naturally undergoes bidirectional transcription yielding a sense and an antisense RNA. PMID: 15979199
    30. CRF treatment results in a clustered distribution of GluRdelta2 along the postsynaptic density, whereas UCN treatment provides a linear distribution. PMID: 16198122
    31. CRF, UCN1, UCN2, and CRF1 and CRF2 receptors play a major role in the regulatory mechanisms that underlie the neural control of gastric motility through CRF2 receptor PMID: 16337313
    32. sustained reduction in body weight and increased responsiveness to subsequent stressors in rats that have been exposed to repeated restraint are not associated with prolonged changes in mRNA expression of CRF receptors or their ligands PMID: 16423352
    33. UCN in lung may act as a possible local autocrine and paracrine immune-inflammatory mediator in inflammatory airway of allergic asthma rats PMID: 16427607
    34. In rats exposed to the social interaction test, urocortin 1 treatment increased the number of c-Fos serotonergic neurons within both the dorsal raphe nucleus and median raphe nucleus. PMID: 16488545
    35. Natural occurrence of a polyadenylated intron-retained urocortin 1 RNA is documented in three brain regions: a midbrain region containing the Edinger-Westphal nucleus, in the cerebellum and in the prefrontal cortex. PMID: 16650605
    36. Our data demonstrate the presence of urocortin and its receptors in rat Kupffer cells, the absence of corticotropin-releasing hormone, and the functionality of these receptors. PMID: 17090973
    37. NPY, via NPY Y5 and to a lesser extent via the Y1 receptors, exerts a stimulatory action on Ucn1 cells in the nonpreganglionic Edinger-Westphal nucleus. PMID: 17154253
    38. Results indicate that the hormonal status of the female rat affects urocortin 1 immunoreactive neurons in the non-preganglionic Edinger-Westphal nucleus and its signaling to target brain areas. PMID: 17391780
    39. Data show in cultured RBE4 cerebral microvessel endothelial cells that urocortin endocytosis is increased by leptin in a time- and dose-dependent manner. PMID: 17478891
    40. Acute UCN treatment protects the heart from reperfusion injury by inhibiting opening of the mitochondrial permeability transition pore, involving a PKC-mediated reduction in oxidative stress. PMID: 17483234
    41. UCN is capable of maintaining adequate nigrostriatal function in vivo, via corticotrophin releasing hormone (CRF)1 receptors following a neuro-inflammatory challenge. PMID: 17659087
    42. endogenous UCN and its receptors might be involved in a complex network of paracrine interaction between dopaminergic neurons and glia PMID: 17947696
    43. Our results suggest that Ucn I in the NTS may play an important role in cardiovascular regulation and the cardiovascular effects of Ucn I may be mediated by activation of both CRF-R1 and -R2, which are known to be present in the NTS. PMID: 18804421
    44. activation of CRF(2)receptor in the solitary nucleus, by Ucn1 and Ucn3, releases glutamate, which, in turn, elicits decreases in blood pressure and heart rate via activation of iGLURs PMID: 19395554

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Sauvagine/corticotropin-releasing factor/urotensin I family
  • 数据库链接：

    KEGG: rno:29151

    STRING: 10116.ENSRNOP00000008037

    UniGene: Rn.10190