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  4. Recombinant Rat Yorkie homolog (Yap1)

Recombinant Rat Yorkie homolog (Yap1)

  • 货号：
    CSB-YP645005RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP645005RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP645005RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP645005RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP645005RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Yap1; Yap; Yap65; Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-469
  • 氨基酸序列
    MEPAQQPPPQ PAPQGPAPPS VSPAGTPAAP PAPPAGHQVV HVRGDSETDL EALFNAVMNP KTANVPQTVP MRLRKLPDSF FKPPEPKSHS RQASTDAGTA GALTPQHVRA HSSPASLQLG AGTLTASGVV SGPAATPAAQ HLRQSSFEIP DDVPLPAGWE MAKTSSGQRY FLNHNDQTTT WQDPRKAMLS QLNVPTSASP AVPQTLMNSA SGPLPDGWEQ AMTQDGEVYY INHKNKTTSW LDPRLDPRFA MNQRITQSAP VKQPPPLAPQ SPQGGVLGGG SSNQQQQIQL QQLQMEKERL RLKQQELFRQ ELALRSQLPS LEQDGGTQNA VSSPGMTQEL RTMTTNSSDP FLNSGTYHSR DESTDSGLSM SSYSIPRTPD DFLNSVDEMD TGDTISQSTL PSQQSRFPDY LEALPGTNVD LGTLEGDAMN IEGEELMPSL QEALSSEILD VESVLAATKL DKESFLTWL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation.; Attenuates p73-mediated cell death signaling in transcriptional repression-induced atypical death (TRIAD) of neurons.; Attenuates p73-mediated cell death signaling in transcriptional repression-induced atypical death (TRIAD) of neurons.; Attenuates p73-mediated cell death signaling in transcriptional repression-induced atypical death (TRIAD) of neurons.
  • 基因功能参考文献：
    1. Here we propose that FGF promotes Hippo/YAP-signaling during lens cell proliferation and differentiation, with FGF-induced nuclear-YAP expression playing an essential role in promoting the proliferation of lens epithelial cells. PMID: 29355736
    2. results suggest that the Hippo-YAP/TAZ signaling pathway participates in periodontal tissue remodeling through various mechanisms PMID: 29356923
    3. YAP1 is protective against VSMC apoptosis induced by ER stress PMID: 28951205
    4. Cell proliferation assay indicated a significant decrease in cell proliferation after Yes-associated protein (YAP) shRNA treatment. PMID: 28915934
    5. YAP modulates cAMP signaling in Sertoli cell which may be critical for testicular function. PMID: 28428044
    6. Our findings identify YAP as a required signaling factor through which fibrous substrate topography influences cell behavior and highlights topography as a key design parameter for engineered biomaterials. PMID: 27889666
    7. Cyclic tensile strain can modulate YAP and ERK activation, which may subsequently regulate cell cycle progression in growth plate chondrocytes. PMID: 26694636
    8. Mechanisms that underlie neuropathic pain remain elusive. We have demonstrated recently that nerve injury can activate Wnt signaling, which becomes a trigger for the development of neuropathic pain. We report that transcriptional regulators YAP and TAZ, which orchestrate Wnt response via incorporation in the beta-catenin destruction complex, are key in the pathogenesis of neuropathic pain and may serve as an "ON-OFF" s... PMID: 27683908
    9. Up-regulation of Yap1 is associated with furan hepatotoxicity. PMID: 27371368
    10. This study identified YAP1 as enhancers of neurite outgrowth. PMID: 26306672
    11. YAP is involved via the Hippo pathway in regeneration and fibrogenesis after ischaemic acute kidney injury. PMID: 26574480
    12. YAP increases the abundance of miR-206, which in turn plays an essential role in mediating hypertrophy and survival by silencing Forkhead box protein P1 in cardiomyocytes. PMID: 26333362
    13. YAP activates the phosphoinositol-3-kinase-AKT pathway and regulates cardiomyocyte proliferation and survival. PMID: 25249570
    14. Report dynamic changes in the Hippo signaling pathway and YAP activation during liver regeneration, which stabilize when the liver-to-body weight ratio reaches homeostatic levels. PMID: 24875096
    15. YAP inactivation is conducive to the maintenance of the chondrogenic cartilage phenotype. PMID: 23543231
    16. Mesenchymal stem cell and chondrocyte fates in response to mechanical cues are regulated by Yes-associated protein. PMID: 23442010
    17. YAP plays a novel integrative role in smooth muscle (SM) phenotypic modulation by inhibiting SM-specific gene expression while promoting SM proliferation and migration in vitro and in vivo. PMID: 22922963
    18. studies demonstrate that Yap1 is a crucial regulator of cardiomyocyte proliferation, cardiac morphogenesis, and myocardial trabeculation PMID: 22308401

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    YAP1 family
  • 组织特异性：
    [Isoform 3]: Highly specific to cortical neurons.
  • 数据库链接：

    KEGG: rno:363014

    UniGene: Rn.162978