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  4. Recombinant Rat cGMP-dependent protein kinase 2 (Prkg2)

Recombinant Rat cGMP-dependent protein kinase 2 (Prkg2)

  • 货号：
    CSB-YP720680RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP720680RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP720680RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP720680RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP720680RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prkg2cGMP-dependent protein kinase 2; cGK 2; cGK2; EC 2.7.11.12; cGMP-dependent protein kinase II; cGKII
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-762
  • 氨基酸序列
    GNGSVKPKH SKHPDGQSGN LSNEALRSKV AELEREVKRK DAELQEREYH LKELREQLAK QTVAIAELTE ELQSKCIQLN KLQDVIHVQG GSPLQASPDK VPLDVHRKTS GLVSLHSRRG AKAGVSAEPT SRTYDLNKPP EFSFEKARVR KDSSEKKLIT DALNKNQFLK RLDPQQIKDM VECMYGRNYQ QGSYIVKQGE PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC TRTASVKAIT NVKTWALDRE VFQNIMRRTA QARDEEYRNF LRSVSLLKNL PEDKLTKIID CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ KGEYFGEKAL ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFDELQKY LEGYVATLNR DDEKRHAKRS MSSWKLSKAL SLEMIQLKEK VARFSSTSPF QNLEIIATLG VGGFGRVELV KVKNENIAFA MKCIRKKHIV DTKQQEHVYS EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS ILRDRGSFDE PTSKFCVACV TEAFDYLHRL GIIYRDLKPE NLILDADGYL KLVDFGFAKK IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF SGIDQMMTYN LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN GINDIKKHRW LNGFNWEGLK ARSLPSPLRR ELSGPIDHSY FDKYPPEKGV PPDEMSGWDK DF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Crucial regulator of intestinal secretion and bone growth. Phosphorylates and activates CFTR on the plasma membrane. Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (Probable). Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863. Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 AND MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation.
  • 基因功能参考文献：
    1. Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not. PMID: 28057484
    2. We conclude that cGKII controls the homeostatic balance of vesicle exocytosis and endocytosis in synaptic boutons of rat cerebellar granule cells. PMID: 24966379
    3. Which in turn activates cGKII and induces the phosphorylation of GluA1. PMID: 23545413
    4. Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway. PMID: 22203739
    5. Inhibition of cGMP-dependent protein kinase II by its own splice isoform PMID: 12054676
    6. results show that the fast phase of autophosphorylation of cGMP dependent protein kinase II has a small effect on its activity, whereas the secondary phase involving Ser126 phosphorylation may generate an active form PMID: 12764134
    7. PKG II activity in zona glomerulosa cells is important for maintaining basal aldosterone production PMID: 12775716
    8. confirmed that BRIN-BD11 cells (and human islets) express all three known isoforms of PKG (PKG-Ialpha, -Ibeta and II) and provide data suggesting that a transcription-dependent pathway of programmed cell death is involved in the actions of cGMP PMID: 14960318
    9. Protein kinase G type II activation may define a critical control point for temporal progression into the daytime domain by acting on the positive arm of the transcriptional/translational feedback loop. PMID: 15312652
    10. role of cGKII as a molecular switch, coupling the cessation of proliferation and the start of hypertrophic differentiation of chondrocytes through attenuation of Sox9 function PMID: 15466490
    11. eNOS, nNOS, cGMP and protein kinase G mediate the inhibitory effect of pancreastatin on growth and proliferation of hepatoma cells PMID: 15582712
    12. While nNOS and cGKI mRNA levels steadily increased during development, cGKII mRNA showed a different behaviour pattern, with similar levels observed on postnatal days 7 and 14 and increased levels noted on postnatal day 21. PMID: 15763176
    13. cGMP and cGK II regulate the translocation of CFTR to the apical membrane in rat jejunum PMID: 15872007
    14. the NO-PKG pathway inhibits AGE-induced proliferation by suppressing activation of JAK2-STAT5 and cyclin D1/cdk4 and induction of p21Waf1/Cip1 PMID: 15958724
    15. Protein kinase G regulation of interleukin 6 promoter signaling pathway largely prevented fluid shear stress-induced increases of IL-6 mRNA in osteosarcoma cells. PMID: 17341596
    16. a subset of phosphorylation sites is modulated, in a cGMP-PKG-specific manner, in intact HEK cells heterologously expressing Cav1.2 alpha(1c) and beta(2a) subunits PMID: 17626895
    17. confirmed mutation in Prkg2 is a valuable model for studying dwarfism and longitudinal growth traits PMID: 19149413

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  • 亚细胞定位：
    Apical cell membrane; Lipid-anchor. Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, cGMP subfamily
  • 组织特异性：
    Highly expressed in intestinal mucosa and is 20 times less abundant in brain and kidney. Expressed in jejunum, in the apical domain of the villus epithelium.
  • 数据库链接：

    KEGG: rno:25523

    STRING: 10116.ENSRNOP00000003237

    UniGene: Rn.87721