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  4. Recombinant Ribonuclease 3 (rnc)

Recombinant Ribonuclease 3 (rnc)

  • 货号：
    CSB-YP816232FMR
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP816232FMR
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP816232FMR-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP816232FMR
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP816232FMR
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rnc
  • Uniprot No.：
  • 别名：
    rnc; SMU_1514; Ribonuclease 3; EC 3.1.26.3; Ribonuclease III; RNase III
  • 种属：
    Streptococcus mutans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-231
  • 氨基酸序列
    MKTLEKKLAE DFKIVFSDKE LLETAFTHTS YANEHRLLNI SHNERLEFLG DAVLQLTISH YLFDKYPQKA EGDLSKMRSM IVREESLAGF SRNCHFDRYI KLGKGEEKSG GRQRDTILGD LFEAFLGALL LDAGLKAVEA FLNQVVIPKV ENNNYERVTD YKTALQELLQ VDGDVLIDYE VLKESGPAHA KCFEVAVSMN HEKLSSGTGK SKKLAEQEAA KNALEKLQRG S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon.; CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA (Probable). Complements pre-crRNA and tracrRNA coprocessing defects in an rnc deletion in S.pyogenes strain 370.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Ribonuclease III family
  • 数据库链接：

    KEGG: smu:SMU_1514

    STRING: 210007.SMU_1514