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  4. Recombinant Rotavirus X Outer capsid protein VP4, partial

Recombinant Rotavirus X Outer capsid protein VP4, partial

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  • 中文名称：
    Recombinant Rotavirus X Outer capsid protein VP4,partial
  • 货号：
    CSB-YP434929RIR
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    ; Outer capsid protein VP4; Hemagglutinin) [Cleaved into: Outer capsid protein VP8*; Outer capsid protein VP5*]
  • 种属：
    Rotavirus X (isolate RVX/Human/Bangladesh/NADRV-B219/2002/GXP[X]) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
  • 蛋白长度：
    Partial
  • 来源：
    Yeast
  • 分子量：
    30.8kDa
  • 表达区域：
    1-249aa
  • 氨基酸序列
    MSLRSLLITTEAVGETTQTSDHQTSFSTRTYNEINDRPSLRVEKDGEKAYCFKNLDPVRYDTRMGEYPFDYGGQSTENNQLQFDLFTKDLMADTDIGLSDDVRDDLKRQIKEYYQQGYRAIFLIRPQNQEQQYIASYSSTNLNFTSQLSVGVNLSVLNKIQENKLHIYSTQPHIPSVGCEMITKIFRTDVDNENSLINYSVPVTVTISVTKATFEDTFVWNQNNDYPNMNYKDLIPAVTKNSIYHDVKR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    This Recombinant Rotavirus X Outer capsid protein VP4 protein was made through genetic engineering. By putting the Outer capsid protein VP4 gene into the genetic material of Yeast cell, the Yeast could be used as factories or producers to make the desired Outer capsid protein VP4 protein for research uses. The expression region of this protein is at 1-249aa. N-terminal 6xHis tag was used in the expression process. The purity is 85%+ determined by SDS-PAGE.

    VP4 is a small (7.5 kDa), linear, hydrophobic peptide that resides on each triangular facet of the capsid interior. VP4 and its precursor VP0 are myristoylated at the NH2 terminus upon virus assembly. Receptor binding induces conformational changes in the virus, including translocation of VP4 and the NH2-terminal region of VP1 to the exterior viral surface. VP1 tethers the particle to the membrane, while myristoylated (Myr) VP4 (MyrVP4) forms a multimeric membrane pore, allowing extrusion of viral RNA genome into the cytoplasm. VP4 is directly involved in mediating membrane penetration during cell entry. Studies utilizing recombinant His-tagged VP4 have shown that VP4 interacts with membranes to make them permeable by formation of multimeric, size-selective membrane pores with properties consistent with the transport of viral genome through the membrane.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. It is subsequently lost, together with VP7, following virus entry into the host cell. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.; Forms the spike 'foot' and 'body' and acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. During entry, the part of VP5* that protrudes from the virus folds back on itself and reorganizes from a local dimer to a trimer. This reorganization may be linked to membrane penetration.; Forms the head of the spikes and mediates the recognition of specific host cell surface glycans. It is the viral hemagglutinin and an important target of neutralizing antibodies.
  • 亚细胞定位：
    [Outer capsid protein VP4]: Virion. Host rough endoplasmic reticulum. Host cell membrane. Host endoplasmic reticulum-Golgi intermediate compartment.
  • 蛋白家族：
    Rotavirus VP4 family