Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Saccharomyces cerevisiae Aspartic proteinase 3 (YPS1)

Recombinant Saccharomyces cerevisiae Aspartic proteinase 3 (YPS1)

  • 货号：
    CSB-YP335717SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP335717SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP335717SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP335717SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP335717SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    YPS1
  • Uniprot No.：
  • 别名：
    YPS1; YAP3; YLR120C; L2961; L9233.9Aspartic proteinase 3; EC 3.4.23.41; Proprotein convertase; Yapsin-1) [Cleaved into: Aspartic proteinase 3 subunit alpha; Aspartic proteinase 3 subunit beta]
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    68-548
  • 氨基酸序列
    ADG YEEIIITNQQ SFYSVDLEVG TPPQNVTVLV DTGSSDLWIM GSDNPYCSSN SMGSSRRRVI DKRDDSSSGG SLINDINPFG WLTGTGSAIG PTATGLGGGS GTATQSVPAS EATMDCQQYG TFSTSGSSTF RSNNTYFSIS YGDGTFASGT FGTDVLDLSD LNVTGLSFAV ANETNSTMGV LGIGLPELEV TYSGSTASHS GKAYKYDNFP IVLKNSGAIK SNTYSLYLND SDAMHGTILF GAVDHSKYTG TLYTIPIVNT LSASGFSSPI QFDVTINGIG ISDSGSSNKT LTTTKIPALL DSGTTLTYLP QTVVSMIATE LGAQYSSRIG YYVLDCPSDD SMEIVFDFGG FHINAPLSSF ILSTGTTCLL GIIPTSDDTG TILGDSFLTN AYVVYDLENL EISMAQARYN TTSENIEIIT SSVPSAVKAP GYTNTWSTSA SIVTGGNIFT VNSSQTASFS GNLTTSTASA TSTSSKRN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cleaves proteins C-terminally to mono- and paired-basic residues. Involved in the shedding of a subset of GPI-anchored plasma membrane proteins from the cell surface, including itself, GAS1 and MSB2. May also play a role in the maturation of GPI-mannoproteins associated with the cell wall. Can process the alpha-mating factor precursor. Required for cell wall integrity.
  • 基因功能参考文献：
    1. Yps1 N-entrance loop plays a major role in regulating the shedding activity of the endopeptidase, most likely by limiting access to the active site, and its cleavage in native Yps1 is associated with its shedding. PMID: 25886139
    2. YPS1 expression is induced during cell wall stress and remodeling in a PKC1-MPK1-dependent manner, indicating that Yps1p is a direct, and important, output of the cell wall integrity response PMID: 16087741
    3. The activation mechanism of GPI-anchored Yps1p was investigated and some of its substrates were identified. PMID: 18573178
    4. Saccharomyces cerevisiae mucin Msb2p is processed into secreted and cell-associated forms, by the aspartyl protease Yps1p. PMID: 18591427
  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor. Note=GPI-anchored plasma membrane protein (GPI-PMP), peripherally distributed over the entire cell surface.
  • 蛋白家族：
    Peptidase A1 family
  • 数据库链接：

    KEGG: sce:YLR120C

    STRING: 4932.YLR120C