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  4. Recombinant Saccharomyces cerevisiae Cell wall integrity sensor MID2 (MID2), partial

Recombinant Saccharomyces cerevisiae Cell wall integrity sensor MID2 (MID2), partial

  • 中文名称：
    酿酒酵母MID2重组蛋白
  • 货号：
    CSB-YP330522SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母MID2重组蛋白
  • 货号：
    CSB-EP330522SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母MID2重组蛋白
  • 货号：
    CSB-EP330522SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母MID2重组蛋白
  • 货号：
    CSB-BP330522SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母MID2重组蛋白
  • 货号：
    CSB-MP330522SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MID2
  • Uniprot No.：
  • 别名：
    MID2; KAI1; SMS1; YLR332W; L8543.19Cell wall integrity sensor MID2; Mating pheromone-induced death protein 2; Protein kinase A interference protein 1; Serine-rich multicopy suppressor protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cell wall stress sensor. Involved in activation of a response that includes both stress-related increased chitin synthesis and the MPK1 mitogen-activated protein kinase cell integrity pathway. High-copy activator of the SKN7 transcription factor. Required during induction of MPK1 tyrosine phosphorylation under a variety of stress conditions. Required for PKC pathway activation under low pH conditions. Required for cell integrity signaling during pheromone-induced morphogenesis. Activates ROM1 or ROM2 catalyzed guanine nucleotide exchange toward RHO1 in cell wall integrity signaling.
  • 基因功能参考文献：
    1. The sensors Wsc1 and Mid2 reside in different plasma membrane compartments in Saccharomyces cerevisiae. PMID: 27337501
    2. As the Mid2p amount plays a role in rgd1Delta sensitivity to low pH, Mid2p seems to act more like a molecular rheostat, controlling the level of PKC pathway activity and thus allowing phenotypical expression of RGD1 inactivation. PMID: 16087742
    3. Chimers of MID2 fused to GFP have been employed to show that intracellular and membrane distribution is only dependent on a membrane-anchored cytoplasmic tail. PMID: 17761172
    4. The extent of the N-linked glycan and its distance from the plasma membrane affect Mid2p function, suggesting the N-glycan to be directly involved in Mid2p sensing. PMID: 18410496
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Note=Localizes uniformly around the cell periphery. Also localized to the mother-bud junction.
  • 蛋白家族：
    MID2 like cell wall stress sensor family
  • 数据库链接：

    KEGG: sce:YLR332W

    STRING: 4932.YLR332W