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  4. Recombinant Saccharomyces cerevisiae Central kinetochore subunit CHL4 (CHL4)

Recombinant Saccharomyces cerevisiae Central kinetochore subunit CHL4 (CHL4)

  • 货号：
    CSB-YP330954SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP330954SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP330954SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP330954SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP330954SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CHL4
  • Uniprot No.：
  • 别名：
    CHL4; CTF17; MCM17; YDR254W; YD9320A.04Inner kinetochore subunit CHL4; CENP-N homolog; Chromosome loss protein 4; Chromosome transmission fidelity protein 17; Constitutive centromere-associated network protein CHL4; Minichromosome maintenance protein 17
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-458
  • 氨基酸序列
    MSNELRLEDN YVPTSDTLVV FKQLMKLPVT VLYDLTLSWF AKFGGSFDGD IYLLTETLDL LIEKGVRRNV IVNRILYVYW PDGLNVFQLA EIDCHLMISK PEKFKWLPSK ALRGDGKPYV VKLQPAKFIE NLQTDLAKIY HCHVYMFKHP SLPVLITRIQ LFDSNNLFLS TPNIGSINKE SLYNKLDKFQ GKPLISRRPY YVAFPLNSPI IFHSVDKDIY ARLVLQSISR TISERETIIF KPVQKIPVKS IHNIMTLLGP SRFAESMGPW ECYASANFER SPLHDYKKHQ GLTGKKVMVR EFDDSFLNDD ENFYGKEEPE IRRLRLEKNM IKFKGSANGV MDQKYNDLKE FNEHVHNIRN GKKNEDSGEP VYISRYSSLV PIEKVGFTLK NEINSRIITI KLKFNGNDIF GGLHELCDKN LINIDKVPGW LAGENGSFSG TIMNGDFQRE QVAKGGLL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.
  • 基因功能参考文献：
    1. s propose that at the core centromere, the Chl4-Iml3 complex participates in recruiting factors, such as Sgo1, that influence sister chromatid cohesion and encourage sister kinetochore biorientation. PMID: 24075991
    2. The study reports the crystal structure of Iml3 from Saccharomyces cerevisiae and further characterizes its interaction with Chl4. PMID: 24311582
  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Associated with kinetochores.
  • 蛋白家族：
    CENP-N/CHL4 family
  • 数据库链接：

    KEGG: sce:YDR254W

    STRING: 4932.YDR254W