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  4. Recombinant Saccharomyces cerevisiae Central kinetochore subunit IML3 (IML3)

Recombinant Saccharomyces cerevisiae Central kinetochore subunit IML3 (IML3)

  • 中文名称：
    酿酒酵母IML3重组蛋白
  • 货号：
    CSB-YP330849SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母IML3重组蛋白
  • 货号：
    CSB-EP330849SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母IML3重组蛋白
  • 货号：
    CSB-EP330849SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母IML3重组蛋白
  • 货号：
    CSB-BP330849SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母IML3重组蛋白
  • 货号：
    CSB-MP330849SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    IML3
  • Uniprot No.：
  • 别名：
    IML3; MCM19; YBR107C; YBR0836; Inner kinetochore subunit IML3; CENP-L homolog; Constitutive centromere-associated network protein IML3; Increased minichromosome loss protein 3; Minichromosome maintenance protein 19
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-245
  • 氨基酸序列
    MPYTWKFLGI SKQLSLENGI AKLNQLLNLE VDLDIQTIRV PSDPDGGTAA DEYIRYEMRL DISNLDEGTY SKFIFLGNSK MEVPMFLCYC GTDNRNEVVL QWLKAEYGVI MWPIKFEQKT MIKLADASIV HVTKENIEQI TWFSSKLYFE PETQDKNLRQ FSIEIPRESC EGLALGYGNT MHPYNDAIVP YIYNETGMAV ERLPLTSVIL AGHTKIMRES IVTSTRSLRN RVLAVVLQSI QFTSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.
  • 基因功能参考文献：
    1. In the absence of Iml3p, the two-hybrid interaction between Ctf19p (a member of the Ctf19 complex) and Dam1p (a member of the outer kinetochore DASH complex) was disrupted and the localization of Dam1p at the kinetochore was also compromised. PMID: 23480593
    2. Aft1 associates with the kinetochore complex through Iml3. PMID: 22157760
    3. Iml3 is essential for sister chromatid segregation in meiosis I and chromosome non-disjunction in meiosis II. PMID: 15241623
  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Associated with kinetochores.
  • 蛋白家族：
    CENP-L/IML3 family
  • 数据库链接：

    KEGG: sce:YBR107C

    STRING: 4932.YBR107C