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  4. Recombinant Saccharomyces cerevisiae Chromatin assembly factor 1 subunit p50 (MSI1)

Recombinant Saccharomyces cerevisiae Chromatin assembly factor 1 subunit p50 (MSI1)

  • 货号：
    CSB-YP320262SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP320262SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP320262SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP320262SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP320262SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MSI1
  • Uniprot No.：
  • 别名：
    MSI1; CAC3; YBR195C; YBR1405; Chromatin assembly factor 1 subunit p50; CAF-1 p50 subunit; IRA1 multicopy suppressor; Protein MSI1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-422
  • 氨基酸序列
    MNQCAKDITH EASSIPIDLQ ERYSHWKKNT KLLYDYLNTN STKWPSLTCQ FFPDLDTTSD EHRILLSSFT SSQKPEDETI YISKISTLGH IKWSSLNNFD MDEMEFKPEN STRFPSKHLV NDISIFFPNG ECNRARYLPQ NPDIIAGASS DGAIYIFDRT KHGSTRIRQS KISHPFETKL FGSHGVIQDV EAMDTSSADI NEATSLAWNL QQEALLLSSH SNGQVQVWDI KQYSHENPII DLPLVSINSD GTAVNDVTWM PTHDSLFAAC TEGNAVSLLD LRTKKEKLQS NREKHDGGVN SCRFNYKNSL ILASADSNGR LNLWDIRNMN KSPIATMEHG TSVSTLEWSP NFDTVLATAG QEDGLVKLWD TSCEETIFTH GGHMLGVNDI SWDAHDPWLM CSVANDNSVH IWKPAGNLVG HS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts. Independently, MSI1 is involved in regulation of the RAS/cAMP pathway via sequestration of NPR1.
  • 基因功能参考文献：
    1. Over-expression of YAK1 decreased the growth rate, but only in the presence of a functional MSI1 gene. PMID: 17321547
    2. Rho5p is a target of Msi1p/Npr1p regulation and the regulatory circuit involving phosphorylation and ubiquitination. PMID: 18621925
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    WD repeat RBAP46/RBAP48/MSI1 family
  • 数据库链接：

    KEGG: sce:YBR195C

    STRING: 4932.YBR195C