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  4. Recombinant Saccharomyces cerevisiae Cysteine protease ATG4 (ATG4)

Recombinant Saccharomyces cerevisiae Cysteine protease ATG4 (ATG4)

  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cysteine protease ATG4(ATG4),Yeast
  • 货号：
    CSB-YP345712SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cysteine protease ATG4(ATG4),Yeast
  • 货号：
    CSB-EP345712SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cysteine protease ATG4(ATG4),Yeast
  • 货号：
    CSB-EP345712SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cysteine protease ATG4(ATG4),Yeast
  • 货号：
    CSB-BP345712SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cysteine protease ATG4(ATG4),Yeast
  • 货号：
    CSB-MP345712SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ATG4
  • Uniprot No.：
  • 别名：
    ATG4; APG4; AUT2; YNL223W; N1274Cysteine protease ATG4; EC 3.4.22.-; Autophagy-related protein 4
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-494
  • 氨基酸序列
    MQRWLQLWKM DLVQKVSHGV FEGSSEEPAA LMNHDYIVLG EVYPERDEES GAEQCEQDCR YRGEAVSDGF LSSLFGREIS SYTKEFLLDV QSRVNFTYRT RFVPIARAPD GPSPLSLNLL VRTNPISTIE DYIANPDCFN TDIGWGCMIR TGQSLLGNAL QILHLGRDFR VNGNESLERE SKFVNWFNDT PEAPFSLHNF VSAGTELSDK RPGEWFGPAA TARSIQSLIY GFPECGIDDC IVSVSSGDIY ENEVEKVFAE NPNSRILFLL GVKLGINAVN ESYRESICGI LSSTQSVGIA GGRPSSSLYF FGYQGNEFLH FDPHIPQPAV EDSFVESCHT SKFGKLQLSE MDPSMLIGIL IKGEKDWQQW KLEVAESAII NVLAKRMDDF DVSCSMDDVE SVSSNSMKKD ASNNENLGVL EGDYVDIGAI FPHTTNTEDV DEYDCFQDIH CKKQKIVVMG NTHTVNANLT DYEVEGVLVE KETVGIHSPI DEKC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Cleaves the C-terminal amino acid of ATG8 to reveal a C-terminal glycine. ATG8 ubiquitin-like activity requires the exposure of the glycine at the C-terminus for its conjugation to phosphatidylethanolamine (PE) and its insertion to membranes, which is necessary for autophagy. The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy. Moreover not only conjugation, but also subsequent ATG8-PE deconjugation performed also by ATG4 is an important step required to delipidate ATG8 to release the protein from membranes, which facilitates multiple events during macroautophagy, and especially for efficient autophagosome biogenesis, the assembly of ATG9-containing tubulovesicular clusters into phagophores/autophagosomes, and for the disassembly of PAS-associated ATG components. ATG8 delipidation by ATG4 also recycles ATG8-PE generated on inappropriate membranes to maintain a reservoir of unlipidated ATG8 that is required for autophagosome formation at the PAS.
  • 基因功能参考文献：
    1. findings showt Atg4 is recruited onto autophagosomal membranes by direct binding to Atg8 via two evolutionarily conserved Atg8 recognition sites; propose a model where Atg4 activity on autophagosomal membranes depends on cooperative action of at least 2 sites within Atg4, in which one functions as a constitutive Atg8 binding module, while the other has a preference toward phosphatidylethanolamine-bound Atg8 PMID: 28330855
    2. Results provide evidence that Atg4 plays an important role in efficient expansion of autophagic isolation membranes by cleaving lipidated Atg8 in Saccharomyces cerevisiae. PMID: 28704456
    3. fine-tuning of Atg4 activity depending on the intracellular redox state may regulate autophagosome formation PMID: 25483965
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Peptidase C54 family
  • 数据库链接：

    KEGG: sce:YNL223W

    STRING: 4932.YNL223W