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  4. Recombinant Saccharomyces cerevisiae DASH complex subunit DAD1 (DAD1)

Recombinant Saccharomyces cerevisiae DASH complex subunit DAD1 (DAD1)

  • 中文名称：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAD1(DAD1),Yeast
  • 货号：
    CSB-YP622455SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAD1(DAD1),Yeast
  • 货号：
    CSB-EP622455SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAD1(DAD1),Yeast
  • 货号：
    CSB-EP622455SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAD1(DAD1),Yeast
  • 货号：
    CSB-BP622455SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAD1(DAD1),Yeast
  • 货号：
    CSB-MP622455SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DAD1; YDR016CDASH complex subunit DAD1; DUO1 and DAM1-interacting protein 1; Outer kinetochore protein DAD1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-94
  • 氨基酸序列
    MMASTSNDEE KLISTTDKYF IEQRNIVLQE INETMNSILN GLNGLNISLE SSIAVGREFQ SVSDLWKTLY DGLESLSDEA PIDEQPTLSQ SKTK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.
  • 基因功能参考文献：
    1. the work presented here shows that are differences between Dad1 from C. albicans and S. cerevisiae. PMID: 20727855
    2. The expression, solubilization, purification and refolding of Dad1p, one of the Dam1-DASH complex subunits, is reported. PMID: 19065263
  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Associates with the mitotic spindle and the kinetochore.
  • 蛋白家族：
    DASH complex DAD1 family
  • 数据库链接：

    KEGG: sce:YDR016C

    STRING: 4932.YDR016C