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  4. Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase II subunit RPB9 (RPB9)

Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase II subunit RPB9 (RPB9)

  • 中文名称：
    酿酒酵母RPB9重组蛋白
  • 货号：
    CSB-YP018336SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RPB9重组蛋白
  • 货号：
    CSB-EP018336SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母RPB9重组蛋白
  • 货号：
    CSB-EP018336SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RPB9重组蛋白
  • 货号：
    CSB-BP018336SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RPB9重组蛋白
  • 货号：
    CSB-MP018336SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RPB9
  • Uniprot No.：
  • 别名：
    RPB9; YGL070C; DNA-directed RNA polymerase II subunit RPB9; RNA polymerase II subunit B9; B12.6; DNA-directed RNA polymerase II 14.2 kDa polypeptide; DNA-directed RNA polymerase II subunit 9
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-122
  • 氨基酸序列
    MTTFRFCRDC NNMLYPREDK ENNRLLFECR TCSYVEEAGS PLVYRHELIT NIGETAGVVQ DIGSDPTLPR SDRECPKCHS RENVFFQSQQ RRKDTSMVLF FVCLSCSHIF TSDQKNKRTQ FS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template. Involved in the regulation of transcription elongation. Involved in DNA repair of damage in the transcribed strand. Mediates a transcription-coupled repair (TCR) subpathway of nucleotide excision repair (NER).
  • 基因功能参考文献：
    1. conclude that Rpb9 supports intra-pol II interactions that modulate TL function and thus pol II enzymatic properties PMID: 27226557
    2. Intrinsic transcript cleavage of Pol II requires the Rpb9 subunit. This enhances the backtrack recovery of Pol II. PMID: 26929337
    3. Rpb9 plays an important role in maintaining transcriptional fidelity, whereas TFIIS may serve a different primary purpose PMID: 16492753
    4. Rad26-mediated repair can be either transcription-coupled or transcription-independent; Rpb9-mediated repair is strictly transcription-coupled and is efficient only when the transcription level is high PMID: 17023424
    5. The transcription elongation function of Rpb9 is involved in transcription-coupled DNA repair. PMID: 17030604
    6. both S-II and Rpb9 maintain transcriptional fidelity by stimulating the cleavage activity intrinsic to RNA polymerase II PMID: 17535246

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  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    Archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family
  • 数据库链接：

    KEGG: sce:YGL070C

    STRING: 4932.YGL070C