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  4. Recombinant Saccharomyces cerevisiae DNA polymerase delta subunit 3 (POL32)

Recombinant Saccharomyces cerevisiae DNA polymerase delta subunit 3 (POL32)

  • 货号：
    CSB-YP018305SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018305SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018305SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018305SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018305SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    POL32
  • Uniprot No.：
  • 别名：
    POL32; YJR043C; J1626DNA polymerase delta subunit 3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-350
  • 氨基酸序列
    MDQKASYFIN EKLFTEVKPV LFTDLIHHLK IGPSMAKKLM FDYYKQTTNA KYNCVVICCY KDQTIKIIHD LSNIPQQDSI IDCFIYAFNP MDSFIPYYDI IDQKDCLTIK NSYELKVSES SKIIERTKTL EEKSKPLVRP TARSKTTPEE TTGRKSKSKD MGLRSTALLA KMKKDRDDKE TSRQNELRKR KEENLQKINK QNPEREAQMK ELNNLFVEDD LDTEEVNGGS KPNSPKETDS NDKDKNNDDL EDLLETTAED SLMDVPKIQQ TKPSETEHSK EPKSEEEPSS FIDEDGYIVT KRPATSTPPR KPSPVVKRAL SSSKKQETPS SNKRLKKQGT LESFFKRKAK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.
  • 基因功能参考文献：
    1. Rev3 role in Pol32 regulation in the course of mutagenesis PMID: 24819597
    2. A rad3/XPD mutation causes TFIIH retention at DNA damage and reduces gap filling. This rad3/XPD mutation causes replication fork breakage and double strand breakes rescued by Rad51- or Pol32-dependent recombination. PMID: 20227372
    3. Strains deficient in Pol32 show little bypass of the T-T (6-4) photoadduct or the abasic sites. PMID: 15520252
    4. Deletion of PIF1 suppresses the lethality of a DNA2-null mutant. Methylmethane sulfonate sensitivity and temperature sensitivity of the pif1delta dna2delta strain can be suppressed by further deletion of a subunit of pol delta, POL32. PMID: 16537895
    5. Pol32, dispensable for replication and for gene conversion, is uniquely required for break-induced replication PMID: 17671506
    6. Unlike Polzeta, inactivation of Pol32 does not result in decreased spontaneous mutagenesis, nor does it limit genome instability in the absence of the error-free postreplication repair pathway. PMID: 17681555
    7. deletion of POL32, which encodes a third, nonessential subunit of polymerase delta, significantly reduced the efficiency of break-induced replication PMID: 18689895
    8. The Pol32 subunit of the major replicative polymerase Poldelta is required for all Segmental duplications(SD) formation, demonstrating that SDs result from untimely DNA synthesis rather than from unequal crossing-over. PMID: 18773114
    9. Results suggest that longer telomeres provide a more proficient substrate for a novel telomere maintenance mechanism that does not rely on telomerase, RAD52, or POL32. PMID: 19380905
    10. Translocations rely on homologous recombination (HR) proteins, such as DNA repair enzymes, as well as on the break-induced replication-specific protein Pol32 and on Srs2. PMID: 19651902

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: sce:YJR043C

    STRING: 4932.YJR043C