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  4. Recombinant Saccharomyces cerevisiae DNA repair protein RAD10 (RAD10)

Recombinant Saccharomyces cerevisiae DNA repair protein RAD10 (RAD10)

  • 中文名称：
    酿酒酵母RAD10重组蛋白
  • 货号：
    CSB-YP361963SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RAD10重组蛋白
  • 货号：
    CSB-EP361963SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母RAD10重组蛋白
  • 货号：
    CSB-EP361963SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RAD10重组蛋白
  • 货号：
    CSB-BP361963SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RAD10重组蛋白
  • 货号：
    CSB-MP361963SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD10
  • Uniprot No.：
  • 别名：
    RAD10; YML095C; DNA repair protein RAD10
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-210
  • 氨基酸序列
    MNNTDPTSFE SILAGVAKLR KEKSGADTTG SQSLEIDASK LQQQEPQTSR RINSNQVINA FNQQKPEEWT DSKATDDYNR KRPFRSTRPG KTVLVNTTQK ENPLLNHLKS TNWRYVSSTG INMIYYDYLV RGRSVLFLTL TYHKLYVDYI SRRMQPLSRN ENNILIFIVD DNNSEDTLND ITKLCMFNGF TLLLAFNFEQ AAKYIEYLNL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Along with RAD1 forms an endonuclease that specifically degrades single-stranded DNA.
  • 基因功能参考文献：
    1. Rad1-Rad10 makes distinct contributions to inter-strand crosslink repair (ICLR) depending on cell cycle phase: in G1, Rad1-Rad10 removes ICL via non-nucleotide excision repair (NER), whereas in S/G2, Rad1-Rad10 facilitates NER-independent replication-coupled. Results suggest that NER function in ICLR is conserved from yeast to human and yeast will provide a tractable system to dissect this critical repair pathway. PMID: 29795289
    2. Presented is a model for the role of Rad1-Rad10 cleavage in formation of recombinants between dispersed repeats. PMID: 22885325
    3. yeast Nucleotide Excision Repair protein assembly is step-wise whereas Rad14 required to recruit Rad10 and Rad14 involvement is transient PMID: 20546858
    4. Rad1-Rad10 functions at different stages of GCR formation and there is an alternative pathway for the GCR formation that is independent of Rad1-Rad10 PMID: 15687264
    5. The ability of Rad1-Rad10 nuclease to associate in a complex with Rad14 is paramount for the targeting of this nuclease to lesion sites in vivo. PMID: 16428464
    6. The Rad10 along with Rad1 and Slx4 promotes efficient repair during gene conversion events involving a single 3' nonhomologous tail PMID: 18579504
    7. Taken together, these results suggest that radiation-induced microhomology-mediated recombination is under the same genetic control as the single-strand annealing apparatus that requires the RAD52, RAD1 and RAD10 genes. PMID: 19630519
    8. observations are consistent with a putative role of Rad10 protein in excising overhanging DNA ends after homology searching and refine the potential role(s) of the Rad1-Rad10 complex in DSB repair in yeast. PMID: 19729509

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ERCC1/RAD10/SWI10 family
  • 数据库链接：

    KEGG: sce:YML095C

    STRING: 4932.YML095C