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  4. Recombinant Saccharomyces cerevisiae Epsin-1 (ENT1)

Recombinant Saccharomyces cerevisiae Epsin-1 (ENT1)

  • 货号：
    CSB-YP619610SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP619610SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP619610SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP619610SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP619610SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ENT1
  • Uniprot No.：
  • 别名：
    ENT1; YDL161WEpsin-1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-454
  • 氨基酸序列
    MSKQFVRSAK NLVKGYSSTQ VLVRNATSND NHQVSKDSLI ELAEKSYDSA DFFEIMDMLD KRLNDKGKYW RHIAKALTVI DYLIRFGSEN CVLWCRENLY IIKTLKEFRH EDDEGIDQGQ IVRVKAKELT ALLSDDERLN EERNMNIKGR NRKGRRRRGT GRSDENDDDL QRAISASRLT AEEDERRRKQ DEDYETALQL SKEEEELKRL QDLQRMQQQQ GQQQLQQPMY YDIFGNPITP EEYAQFQLQQ QQQQQQQQLQ QQPMYYDVFG NPITPEELAQ FQQQQQLQEQ QYLASMQQQQ QAMSNNPFAK SEQSSSSPKR NQLVAASSPQ QLQQQKQQEP LIQNRTGNQS MTDKYSKLNE LLATGTGIDT FGNVGEARIP AQHTKTGTFI NSQGTGYRQV SDDPNHNPFL NSQYTGLPST SVVPTQTGYG FGNQSQQQSQ NNGSNNRGYT LIDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Negatively regulated via phosphorylation.
  • 基因功能参考文献：
    1. X-ray analysis revealed that the crystallographic symmetry of Ent1 is primitive orthorhombic, space group P222, with unit-cell parameters a = 32.7, b = 35.5, c = 110.6 A and a diffraction limit of 2.3 A PMID: 22750874
    2. Epsin/Yap180 adaptor interaction with the scaffolds Ede1/Pan1 is required for endocytosis PMID: 18448668
  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein. Note=Localizes in a punctate pattern. Found in the actin cortical patches.
  • 蛋白家族：
    Epsin family
  • 数据库链接：

    KEGG: sce:YDL161W

    STRING: 4932.YDL161W