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  4. Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 5 (TIF5)

Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 5 (TIF5)

  • 货号：
    CSB-YP007572SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007572SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007572SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007572SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007572SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TIF5
  • Uniprot No.：
  • 别名：
    TIF5; YPR041W; YP3085.05Eukaryotic translation initiation factor 5; eIF-5
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-405
  • 氨基酸序列
    MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVLD GFINKFVLCG SCKNPETEII ITKDNDLVRD CKACGKRTPM DLRHKLSSFI LKNPPDSVSG SKKKKKAATA SANVRGGGLS ISDIAQGKSQ NAPSDGTGSS TPQHHDEDED ELSRQIKAAA STLEDIEVKD DEWAVDMSEE AIRARAKELE VNSELTQLDE YGEWILEQAG EDKENLPSDV ELYKKAAELD VLNDPKIGCV LAQCLFDEDI VNEIAEHNAF FTKILVTPEY EKNFMGGIER FLGLEHKDLI PLLPKILVQL YNNDIISEEE IMRFGTKSSK KFVPKEVSKK VRRAAKPFIT WLETAESDDD EEDDE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). eIF-5 is essential for cell viability.
  • 基因功能参考文献：
    1. eIF2B has a second activity as a GDI displacement factor (GDF) that can recruit eIF2 from the eIF2*GDP/eIF5 GDI complex prior to GEF action. PMID: 24352424
    2. eIF5 is critical for integrity of the scanning preinitiation complex and accurate control of GCN4 translation. PMID: 15964804
    3. Data suggest strongly that the interaction between eIF5 and eIF1A is involved in maintaining the fidelity of start codon AUG recognition in vivo. PMID: 16380131
    4. Results describe the 1.5A crystal structure of eukaryotic initiation factor eIF5 C terminal domain, confirming that eIF5-CTD contains an atypical HEAT motif. PMID: 16616930
    5. eIF-5 can form the multifactor complex with the ribosome 40S subunit. PMID: 17389391
    6. The direct interaction at eIF1 places eIF5 near the decoding site of the 40 S subunit. PMID: 17974565

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  • 蛋白家族：
    EIF-2-beta/eIF-5 family
  • 数据库链接：

    KEGG: sce:YPR041W

    STRING: 4932.YPR041W