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  4. Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor eIF-1 (SUI1)

Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor eIF-1 (SUI1)

  • 货号：
    CSB-YP330209SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP330209SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP330209SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP330209SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP330209SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SUI1
  • Uniprot No.：
  • 别名：
    SUI1; RFR1; YNL244C; N0905; Eukaryotic translation initiation factor eIF-1; Protein translation factor SUI1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-108
  • 氨基酸序列
    SIENLKSFD PFADTGDDET ATSNYIHIRI QQRNGRKTLT TVQGVPEEYD LKRILKVLKK DFACNGNIVK DPEMGEIIQL QGDQRAKVCE FMISQLGLQK KNIKIHGF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.
  • 基因功能参考文献：
    1. Thus, electrostatic interactions involving the eIF1A N-terminal tail stabilize the closed conformation and promote utilization of suboptimal start codons. PMID: 29206102
    2. eIF1 Loop 2 interactions with Met-tRNAi control the accuracy of start codon selection by the scanning preinitiation complex. PMID: 29666249
    3. The GTPase-activating protein eIF5 and beta-subunit of eIF2 promote transcriptional accuracy by controlling eIF1 dissociation and the stability of ternary complex binding to the preinitiation complex. PMID: 24335188
    4. substituting Lys-60 in helix alpha1, or either Lys-37 or Arg-33 in beta-hairpin loop-1, impairs binding of yeast eIF1 to 40 S.eIF1A complexes in vitro, and it confers increased initiation at UUG codons (Sui(-) phenotype) PMID: 23893413
    5. Data show that start codon recognition results in a conformational change and release of eIF1 from the ribosome. PMID: 15664195
    6. EIF-1 can form the multifactor complex with the ribosome 40S subunit. PMID: 17389391
    7. Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo. PMID: 17504939

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  • 蛋白家族：
    SUI1 family
  • 数据库链接：

    KEGG: sce:YNL244C

    STRING: 4932.YNL244C